Construct: ORF TRCN0000472788
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008003.4_s317c1
- Derived from:
- ccsbBroadEn_14930
- DNA Barcode:
- ACCGTGTACAGCAGCTCTCGGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LATS1 (9113)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472788
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9113 | LATS1 | large tumor suppressor kina... | NM_001270519.1 | 99.9% | 100% | 1446T>C |
| 2 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_017011482.1 | 97.2% | 97.1% | (many diffs) |
| 3 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_006715603.3 | 76.9% | 76.4% | (many diffs) |
| 4 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_017011479.1 | 68.6% | 68.1% | (many diffs) |
| 5 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_011536252.2 | 68.1% | 67.6% | (many diffs) |
| 6 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_017011477.1 | 65.9% | 65.4% | (many diffs) |
| 7 | human | 9113 | LATS1 | large tumor suppressor kina... | NM_004690.4 | 60.3% | 59.9% | (many diffs) |
| 8 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_017011474.1 | 58.6% | 58.2% | (many diffs) |
| 9 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_024446583.1 | 58.6% | 58.2% | (many diffs) |
| 10 | human | 9113 | LATS1 | large tumor suppressor kina... | NM_001350340.2 | 50.8% | 49.9% | (many diffs) |
| 11 | human | 9113 | LATS1 | large tumor suppressor kina... | NM_001350339.2 | 49.4% | 48.4% | (many diffs) |
| 12 | human | 9113 | LATS1 | large tumor suppressor kina... | NM_001350392.2 | 35.6% | 35.1% | (many diffs) |
| 13 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_017011480.1 | 34.6% | 34.1% | (many diffs) |
| 14 | human | 9113 | LATS1 | large tumor suppressor kina... | NR_073033.1 | 27.5% | (many diffs) | |
| 15 | mouse | 16798 | Lats1 | large tumor suppressor | XM_006512567.1 | 84.3% | 87.5% | (many diffs) |
| 16 | mouse | 16798 | Lats1 | large tumor suppressor | NM_010690.1 | 52.5% | 54% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2136
- ORF length:
- 2070
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaggagtgaa aagccagaag gatatagaca aatgaggcct aagacctttc 121 ctgccagtaa ctatactgtc agtagccggc aaatgttaca agaaattcgg gaatccctta 181 ggaatttatc taaaccatct gatgctgcta aggctgagca taacatgagt aaaatgtcaa 241 ccgaagatcc tcgacaagtc agaaatccac ccaaatttgg gacgcatcat aaagccttgc 301 aggaaattcg aaactctctg cttccatttg caaatgaaac aaattcttct cggagtactt 361 cagaagttaa tccacaaatg cttcaagact tgcaagctgc tggatttgat gaggatatgg 421 ttatacaagc tcttcagaaa actaacaaca gaagtataga agcagcaatt gaattcatta 481 gtaaaatgag ttaccaagat cctcgacgag agcagatggc tgcagcagct gccagaccta 541 ttaatgccag catgaaacca gggaatgtgc agcaatcagt taaccgcaaa cagagctgga 601 aaggttctaa agaatcctta gttcctcaga ggcatggccc gccactagga gaaagtgtgg 661 cctatcattc tgagagtccc aactcacaga cagatgtagg aagacctttg tctggatctg 721 gtatatcagc atttgttcaa gctcacccta gcaacggaca gagagtgaac cccccaccac 781 cacctcaagt aaggagtgtt actcctccac cacctccaag aggccagact ccccctccaa 841 gaggtacaac tccacctccc ccttcatggg aaccaaactc tcaaacaaag cgctattctg 901 gaaacatgga atacgtaatc tcccgaatct ctcctgtccc acctggggca tggcaagagg 961 gctatcctcc accacctctc aacacttccc ccatgaatcc tcctaatcaa ggacagagag 1021 gcattagttc tgttcctgtt ggcagacaac caatcatcat gcagagttct agcaaattta 1081 actttccatc agggagacct ggaatgcaga atggtactgg acaaactgat ttcatgatac 1141 accaaaatgt tgtccctgct ggcactgtga atcggcagcc accacctcca tatcctctga 1201 cagcagctaa tggacaaagc ccttctgctt tacaaacagg gggatctgct gctccttcgt 1261 catatacaaa tggaagtatt cctcagtcta tgatggtgcc aaacagaaat agtcataaca 1321 tggaactata taacattagt gtacctggac tgcaaacaaa ttggcctcag tcatcttctg 1381 ctccagccca gtcatccccg agcagtgggc atgaaatccc tacatggcaa cctaacatac 1441 cagtgaggtc aaattctttt aataacccat taggaaatag agcaagtcac tctgctaatt 1501 ctcagccttc cgctacaaca gtcactgcaa ttacaccagc tcctattcaa cagcctgtga 1561 aaagtatgcg tgtattaaaa ccagagctac agactgcttt agcacctaca cacccttctt 1621 ggataccaca gccaattcaa actgttcaac ccagtccttt tcctGAGGGA ACCGCTTCAA 1681 ATGTGACTGT GATGCCACCT GTTGCTGAAG CTCCAAACTA TCAAGGACCA CCACCACCCT 1741 ACCCAAAACA TCTGCTGCAC CAAAACCCAT CTGTTCCTCC ATACGAGTCA ATCAGTAAGC 1801 CTAGCAAAGA GGATCAGCCA AGCTTGCCCA AGGAAGATGA GAGTGAAAAG AGTTATGAAA 1861 ATGTTGATAG TGGGGATAAA GAAAAGAAAC AGATTACAAC TTCACCTATT ACTGTTAGGA 1921 AAAACAAGAA AGATGAAGAG CGAAGGGAAT CTCGTATTCA AAGTTATTCT CCTCAAGCAT 1981 TTAAATTCTT TATGGAGCAA CATGTAGAAA ATGTACTCAA ATCTCATCAG CAGCGTCTAC 2041 ATCGTAAAAA ACAATTAGAG AATGAAATGA TGCGGGTAAA ACCTTTTAAA ATGTCCATTT 2101 TTATACTTAA TCATCTGTTT GCTTGGTGTT TATTTTACCC AACTTTCTTG TACAAAGTGG 2161 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2221 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2281 AACCGTGTAC AGCAGCTCTC GGTACACGCG TTAAGTCgac aatcaacctc tggattacaa 2341 aatttgtgaa agatt