Construct: ORF TRCN0000472863
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011932.1_s317c1
- Derived from:
- ccsbBroadEn_11776
- DNA Barcode:
- GTTATCTTATAACGAACTAGGTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SUMF2 (25870)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472863
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25870 | SUMF2 | sulfatase modifying factor 2 | NM_001366649.2 | 30.9% | 7.4% | 1_95del;255_513del |
| 2 | human | 25870 | SUMF2 | sulfatase modifying factor 2 | NM_001146333.2 | 24.8% | 24.8% | 1_480del |
| 3 | human | 25870 | SUMF2 | sulfatase modifying factor 2 | NM_001042470.3 | 24.4% | 24.4% | 1_492del |
| 4 | human | 25870 | SUMF2 | sulfatase modifying factor 2 | NM_001042469.3 | 18.5% | 18.5% | 1_699del |
| 5 | human | 25870 | SUMF2 | sulfatase modifying factor 2 | NM_015411.4 | 17.6% | 17.6% | 1_744del |
| 6 | human | 25870 | SUMF2 | sulfatase modifying factor 2 | NM_001042468.3 | 17.4% | 17.4% | 1_753del |
| 7 | human | 25870 | SUMF2 | sulfatase modifying factor 2 | NM_001366647.2 | 15.7% | 10% | 1_699del;774_891del;977_1011del |
| 8 | human | 25870 | SUMF2 | sulfatase modifying factor 2 | NM_001366648.2 | 15% | 9.6% | 1_744del;819_936del;1022_1056del |
| 9 | human | 25870 | SUMF2 | sulfatase modifying factor 2 | XM_011515254.3 | 12.4% | 9.8% | (many diffs) |
| 10 | human | 25870 | SUMF2 | sulfatase modifying factor 2 | XR_001744617.1 | 7.2% | 1_853del;928_1045del;1131_2191del | |
| 11 | human | 25870 | SUMF2 | sulfatase modifying factor 2 | XR_001744616.1 | 7.1% | 1_898del;973_1090del;1176_2236del | |
| 12 | human | 25870 | SUMF2 | sulfatase modifying factor 2 | XR_002956418.1 | 7% | 1_1030del;1190_2250del | |
| 13 | mouse | 67902 | Sumf2 | sulfatase modifying factor 2 | NM_026445.2 | 15% | 15.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 247
- ORF end:
- 406
- ORF length:
- 159
- Sequence:
-
1 GGAGTACGTC GTCTTTAGGT TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG 61 AGTGGGTGGA GACTGAAGTT AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG 121 CCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT 181 TTCTTCCATT TCAGGTGTCG TGAGGCTAGC ATCGATTGAT CAACAAGTTT GTACAAAAAA 241 GTTGGCATGC GCGTCCTCCG GGGGGCATCC TGGATCGACA CAGCTGATGG CTCTGCCAAT 301 CACCGGGCCC GGGTCACCAC CAGGATGGGC AACACTCCAG ATTCAGCCTC AGACAACCTC 361 GGTTTCCGCT GTGCTGCAGA CGCAGGCCGG CCGCCAGGGG AGCTGTACCC AACTTTCTTG 421 TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG 481 TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT 541 GGAAAGGACG AGTTATCTTA TAACGAACTA GGTGTACGCG TTAAGTCgac aatcaacctc 601 tggattacaa aatttgtgaa agatt