Transcript: Human NM_001146333.2

Homo sapiens sulfatase modifying factor 2 (SUMF2), transcript variant 7, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
SUMF2 (25870)
Length:
1835
CDS:
134..775

Additional Resources:

NCBI RefSeq record:
NM_001146333.2
NBCI Gene record:
SUMF2 (25870)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001146333.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000140005 GTGTTACACGTGAGCTGGAAT pLKO.1 299 CDS 100% 4.950 6.930 N SUMF2 n/a
2 TRCN0000139433 CAGAACAACTACGGGCTCTAT pLKO.1 533 CDS 100% 4.950 3.960 N SUMF2 n/a
3 TRCN0000140208 GAGCACTCTGAAAGGCCATTT pLKO.1 1068 3UTR 100% 10.800 7.560 N SUMF2 n/a
4 TRCN0000140400 GCTGTGGAAGGAGAATGCTTT pLKO.1 1198 3UTR 100% 4.950 3.465 N SUMF2 n/a
5 TRCN0000139550 CCATGTTGCAAACAGCGCAAT pLKO.1 830 3UTR 100% 4.050 2.835 N SUMF2 n/a
6 TRCN0000142563 GAGCTTTGTCTTTGAGGACTT pLKO.1 145 CDS 100% 4.050 2.835 N SUMF2 n/a
7 TRCN0000140102 GTATCGGACAGAAGCTGAGAT pLKO.1 115 5UTR 100% 4.950 2.970 N SUMF2 n/a
8 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 1684 3UTR 100% 5.625 2.813 Y KLHL30 n/a
9 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 1684 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001146333.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11775 pDONR223 100% 45.9% 43.3% None 0_1ins177;412_556del;639_640ins259 n/a
2 ccsbBroad304_11775 pLX_304 0% 45.9% 43.3% V5 0_1ins177;412_556del;639_640ins259 n/a
3 TRCN0000466659 TGGGATTTACACAAACAACACGCT pLX_317 34.4% 45.9% 43.3% V5 0_1ins177;412_556del;639_640ins259 n/a
4 ccsbBroadEn_11776 pDONR223 100% 24.8% 24.8% None 1_480del n/a
5 ccsbBroad304_11776 pLX_304 0% 24.8% 24.8% V5 1_480del n/a
6 TRCN0000472863 GTTATCTTATAACGAACTAGGTGT pLX_317 100% 24.8% 24.8% V5 1_480del n/a
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