Construct: ORF TRCN0000472892
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018082.1_s317c1
- Derived from:
- ccsbBroadEn_00970
- DNA Barcode:
- GCGCCTCGAAAGTTGTCGTGATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAGOH (4116)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472892
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4116 | MAGOH | mago homolog, exon junction... | NM_002370.4 | 100% | 100% | |
| 2 | human | 55110 | MAGOHB | mago homolog B, exon juncti... | NM_018048.5 | 85.3% | 97.2% | (many diffs) |
| 3 | human | 100506898 | MAGOH2P | mago homolog 2, pseudogene | NR_049723.1 | 64.5% | (many diffs) | |
| 4 | human | 55110 | MAGOHB | mago homolog B, exon juncti... | NM_001300739.2 | 60.7% | 69.8% | (many diffs) |
| 5 | human | 55110 | MAGOHB | mago homolog B, exon juncti... | NM_001319985.1 | 60.7% | 69.8% | (many diffs) |
| 6 | human | 55110 | MAGOHB | mago homolog B, exon juncti... | XM_005253402.2 | 60.7% | 69.8% | (many diffs) |
| 7 | human | 55110 | MAGOHB | mago homolog B, exon juncti... | NR_135120.1 | 13% | (many diffs) | |
| 8 | mouse | 17149 | Magoh | mago homolog, exon junction... | NM_001282737.1 | 91% | 100% | (many diffs) |
| 9 | mouse | 66441 | Magohb | mago homolog B, exon juncti... | NM_025564.2 | 82.6% | 97.9% | (many diffs) |
| 10 | mouse | 66441 | Magohb | mago homolog B, exon juncti... | NM_001327860.1 | 58.6% | 69.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 528
- ORF length:
- 438
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGAGAGTGA CTTTTATCTG CGTTACTACG 121 TGGGGCACAA GGGCAAGTTC GGCCACGAGT TCCTGGAGTT TGAGTTTCGA CCGGACGGGA 181 AGTTAAGATA TGCCAACAAC AGCAATTACA AGAATGATGT CATGATCAGA AAAGAGGCTT 241 ATGTACATAA AAGCGTGATG GAGGAACTGA AGAGAATAAT TGACGACAGT GAAATTACCA 301 AAGAGGATGA TGCATTGTGG CCTCCTCCTG ACCGAGTGGG CCGGCAGGAG CTTGAAATCG 361 TCATTGGAGA TGAACACATT TCTTTTACAA CATCAAAAAT TGGTTCCCTT ATTGATGTCA 421 ATCAATCCAA GGATCCAGAA GGCTTACGAG TATTTTATTA TCTTGTCCAG GACCTGAAGT 481 GTTTGGTCTT CAGTCTTATT GGATTACACT TCAAGATTAA ACCAATCTAC CCAACTTTCT 541 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 601 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 661 GTGGAAAGGA CGAGCGCCTC GAAAGTTGTC GTGATGTACG CGTTAAGTCg acaatcaacc 721 tctggattac aaaatttgtg aaagatt