Construct: ORF TRCN0000472927

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019006.3_s317c1
Derived from:
ccsbBroadEn_14734
DNA Barcode:
CCCTCGTGGCCGGGGGTTCCCTGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDGFRA (5156)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472927
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5156 PDGFRA platelet derived growth fac... NM_001347829.2 99.9% 100% 3222T>C;3266_3267delTG
2 human 5156 PDGFRA platelet derived growth fac... NM_006206.6 99.9% 100% 3222T>C;3266_3267delTG
3 human 5156 PDGFRA platelet derived growth fac... XM_005265743.1 99.9% 100% 3222T>C;3266_3267delTG
4 human 5156 PDGFRA platelet derived growth fac... NM_006206.4:c.2522_2527del 99.7% 99.8% 2519_2520insCAGAGA;3216T>C;3260_3261delTG
5 human 5156 PDGFRA platelet derived growth fac... NM_006206.4:c.2526_2537del 99.5% 99.6% 2523_2524insGACATCATGCAT;3210T>C;3254_3255delTG
6 human 5156 PDGFRA platelet derived growth fac... NM_001347830.1 98.7% 98.8% 1_39del;3261T>C;3305_3306delTG
7 human 5156 PDGFRA platelet derived growth fac... NM_001347828.2 97.6% 97.7% 1_75del;3297T>C;3341_3342delTG
8 human 5156 PDGFRA platelet derived growth fac... NM_001347827.2 73.2% 70.9% (many diffs)
9 human 5156 PDGFRA platelet derived growth fac... XM_017008281.1 72.4% 70.1% (many diffs)
10 human 5156 PDGFRA platelet derived growth fac... XM_006714041.3 71.6% 69.3% (many diffs)
11 mouse 18595 Pdgfra platelet derived growth fac... NM_001083316.2 86.4% 91.8% (many diffs)
12 mouse 18595 Pdgfra platelet derived growth fac... NM_011058.3 86.4% 91.8% (many diffs)
13 mouse 18595 Pdgfra platelet derived growth fac... XM_006504261.3 86.4% 91.8% (many diffs)
14 mouse 18595 Pdgfra platelet derived growth fac... XM_006504262.3 86.4% 91.8% (many diffs)
15 mouse 18595 Pdgfra platelet derived growth fac... XM_006504263.3 86.4% 91.8% (many diffs)
16 mouse 18595 Pdgfra platelet derived growth fac... NM_001347718.1 61.1% 63.2% (many diffs)
17 mouse 18595 Pdgfra platelet derived growth fac... NM_001347719.1 61.1% 63.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3336
ORF length:
3267
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggggacttcc catccggcgt tcctggtctt aggctgtctt ctcacagggc
121tgagcctaat cctctgccag ctttcattac cctctatcct tccaaatgaa aatgaaaagg
181ttgtgcagct gaattcatcc ttttctctga gatgctttgg ggagagtgaa gtgagctggc
241agtaccccat gtctgaagaa gagagctccg atgtggaaat cagaaatgaa gaaaacaaca
301gcggcctttt tgtgacggtc ttggaagtga gcagtgcctc ggcggcccac acagggttgt
361acacttgcta ttacaaccac actcagacag aagagaatga gcttgaaggc aggcacattt
421acatctatgt gccagaccca gatgtagcct ttgtacctct aggaatgacg gattatttag
481tcatcgtgga ggatgatgat tctgccatta taccttgtcg cacaactgat cccgagactc
541ctgtaacctt acacaacagt gagggggtgg tacctgcctc ctacgacagc agacagggct
601ttaatgggac cttcactgta gggccctata tctgtgaggc caccgtcaaa ggaaagaagt
661tccagaccat cccatttaat gtttatgctt taaaagcaac atcagagctg gatctagaaa
721tggaagctct taaaaccgtg tataagtcag gggaaacgat tgtggtcacc tgtgctgttt
781ttaacaatga ggtggttgac cttcaatgga cttaccctgg agaagtgaaa ggcaaaggca
841tcacaatgct ggaagaaatc aaagtcccat ccatcaaatt ggtgtacact ttgacggtcc
901ccgaggccac ggtgaaagac agtggagatt acgaatgtgc tgcccgccag gctaccaggg
961aggtcaaaga aatgaagaaa gtcactattt ctgtccatga gaaaggtttc attgaaatca
1021aacccacctt cagccagttg gaagctgtca acctgcatga agtcaaacat tttgttgtag
1081aggtgcgggc ctacccacct cccaggatat cctggctgaa aaacaatctg actctgattg
1141aaaatctcac tgagatcacc actgatgtgg aaaagattca ggaaataagg tatcgaagca
1201aattaaagct gatccgtgct aaggaagaag acagtggcca ttatactatt gtagctcaaa
1261atgaagatgc tgtgaagagc tatacttttg aactgttaac tcaagttcct tcatccattc
1321tggacttggt cgatgatcac catggctcaa ctgggggaca gacggtgagg tgcacagctg
1381aaggcacgcc gcttcctgat attgagtgga tgatatgcaa agatattaag aaatgtaata
1441atgaaacttc ctggactatt ttggccaaca atgtctcaaa catcatcacg gagatccact
1501cccgagacag gagtaccgtg gagggccgtg tgactttcgc caaagtggag gagaccatcg
1561ccgtgcgatg cctggctaag aatctccttg gagctgagaa ccgagagctg aagctggtgg
1621ctcccaccct gcgttctgaa ctcacggtgg ctgctgcagt cctggtgctg ttggtgattg
1681tgatcatctc acttattgtc ctggttgtca tttggaaaca gaaaccgagg tatgaaattc
1741gctggagggt cattgaatca atcagcccag atggacatga atatatttat gtggacccga
1801tgcagctgcc ttatgactca agatgggagt ttccaagaga tggactagtg cttggtcggg
1861tcttggggtc tggagcgttt gggaaggtgg ttgaaggaac agcctatgga ttaagccggt
1921cccaacctgt catgaaagtt gcagtgaaga tgctaaaacc cacggccaga tccagtgaaa
1981aacaagctct catgtctgaa ctgaagataa tgactcacct ggggccacat ttgaacattg
2041taaacttgct gggagcctgc accaagtcag gccccattta catcatcaca gagtattgct
2101tctatggaga tttggtcaac tatttgcata agaataggga tagcttcctg agccaccacc
2161cagagaagcc aaagaaagag ctggatatct ttggattgaa ccctgctgat gaaagcacac
2221ggagctatgt tattttatct tttgaaaaca atggtgacta catggacatg aagcaggctg
2281atactacaca gtatgtcccc atgctagaaa ggaaagaggt ttctaaatat tccgacatcc
2341agagatcact ctatgatcgt ccagcctcat ataagaagaa atctatgtta gactcagaag
2401tcaaaaacct cctttcagat gataactcag aaggccttac tttattggat ttgttgagct
2461tcacctatca agttgcccga ggaatggagt ttttggcttc aaaaaattgt gtccaccgtg
2521atctggctgc tcgcaacgtc ctcctggcac aaggaaaaat tgtgaagatc tgtgactttg
2581gcctggccag agacatcatg catgattcga actatgtgtc gaaaggcagt acctttctgc
2641ccgtgaagtg gatggctcct gagagcatct ttgacaacct ctacaccaca ctgagtgatg
2701tctggtctta tggcattctg ctctgggaga tcttttccct tggtggcacc ccttaccccg
2761gcatgatggt ggattctact ttctacaata agatcaagag tgggtaccgg atggccaagc
2821ctgaccacgc taccagtgaa gtctacgaga tcatggtgaa atgctggaac agtgagccgg
2881agaagagacc ctccttttac caccTGAGTG AGATTGTGGA GAATCTGCTG CCTGGACAAT
2941ATAAAAAGAG TTATGAAAAA ATTCACCTGG ACTTCCTGAA GAGTGACCAT CCTGCTGTGG
3001CACGCATGCG TGTGGACTCA GACAATGCAT ACATTGGTGT CACCTACAAA AACGAGGAAG
3061ACAAGCTGAA GGACTGGGAG GGTGGTCTGG ATGAGCAGAG ACTGAGCGCT GACAGTGGCT
3121ACATCATTCC TCTGCCTGAC ATTGACCCTG TCCCTGAGGA GGAGGACCTG GGCAAGAGGA
3181ACAGACACAG CTCGCAGACC TCTGAAGAGA GTGCCATTGA GACGGGTTCC AGCAGTTCCA
3241CCTTCATCAA GAGAGAGGAC GAGACCATTG AAGACATCGA CATGATGGAC GACATCGGCA
3301TAGACTCTTC AGACCTGGTG GAAGACAGCT TCCTGCCAAC TTTCTTGTAC AAAGTGGTTG
3361ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
3421GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC
3481CTCGTGGCCG GGGGTTCCCT GTACGCGTTA AGTCgacaat caacctctgg attacaaaat
3541ttgtgaaaga tt

Download FASTA (ORF) (Full)