Construct: ORF TRCN0000472927
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019006.3_s317c1
- Derived from:
- ccsbBroadEn_14734
- DNA Barcode:
- CCCTCGTGGCCGGGGGTTCCCTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDGFRA (5156)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472927
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347829.2 | 99.9% | 100% | 3222T>C;3266_3267delTG |
| 2 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_006206.6 | 99.9% | 100% | 3222T>C;3266_3267delTG |
| 3 | human | 5156 | PDGFRA | platelet derived growth fac... | XM_005265743.1 | 99.9% | 100% | 3222T>C;3266_3267delTG |
| 4 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_006206.4:c.2522_2527del | 99.7% | 99.8% | 2519_2520insCAGAGA;3216T>C;3260_3261delTG |
| 5 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_006206.4:c.2526_2537del | 99.5% | 99.6% | 2523_2524insGACATCATGCAT;3210T>C;3254_3255delTG |
| 6 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347830.1 | 98.7% | 98.8% | 1_39del;3261T>C;3305_3306delTG |
| 7 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347828.2 | 97.6% | 97.7% | 1_75del;3297T>C;3341_3342delTG |
| 8 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347827.2 | 73.2% | 70.9% | (many diffs) |
| 9 | human | 5156 | PDGFRA | platelet derived growth fac... | XM_017008281.1 | 72.4% | 70.1% | (many diffs) |
| 10 | human | 5156 | PDGFRA | platelet derived growth fac... | XM_006714041.3 | 71.6% | 69.3% | (many diffs) |
| 11 | mouse | 18595 | Pdgfra | platelet derived growth fac... | NM_001083316.2 | 86.4% | 91.8% | (many diffs) |
| 12 | mouse | 18595 | Pdgfra | platelet derived growth fac... | NM_011058.3 | 86.4% | 91.8% | (many diffs) |
| 13 | mouse | 18595 | Pdgfra | platelet derived growth fac... | XM_006504261.3 | 86.4% | 91.8% | (many diffs) |
| 14 | mouse | 18595 | Pdgfra | platelet derived growth fac... | XM_006504262.3 | 86.4% | 91.8% | (many diffs) |
| 15 | mouse | 18595 | Pdgfra | platelet derived growth fac... | XM_006504263.3 | 86.4% | 91.8% | (many diffs) |
| 16 | mouse | 18595 | Pdgfra | platelet derived growth fac... | NM_001347718.1 | 61.1% | 63.2% | (many diffs) |
| 17 | mouse | 18595 | Pdgfra | platelet derived growth fac... | NM_001347719.1 | 61.1% | 63.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3336
- ORF length:
- 3267
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggacttcc catccggcgt tcctggtctt aggctgtctt ctcacagggc 121 tgagcctaat cctctgccag ctttcattac cctctatcct tccaaatgaa aatgaaaagg 181 ttgtgcagct gaattcatcc ttttctctga gatgctttgg ggagagtgaa gtgagctggc 241 agtaccccat gtctgaagaa gagagctccg atgtggaaat cagaaatgaa gaaaacaaca 301 gcggcctttt tgtgacggtc ttggaagtga gcagtgcctc ggcggcccac acagggttgt 361 acacttgcta ttacaaccac actcagacag aagagaatga gcttgaaggc aggcacattt 421 acatctatgt gccagaccca gatgtagcct ttgtacctct aggaatgacg gattatttag 481 tcatcgtgga ggatgatgat tctgccatta taccttgtcg cacaactgat cccgagactc 541 ctgtaacctt acacaacagt gagggggtgg tacctgcctc ctacgacagc agacagggct 601 ttaatgggac cttcactgta gggccctata tctgtgaggc caccgtcaaa ggaaagaagt 661 tccagaccat cccatttaat gtttatgctt taaaagcaac atcagagctg gatctagaaa 721 tggaagctct taaaaccgtg tataagtcag gggaaacgat tgtggtcacc tgtgctgttt 781 ttaacaatga ggtggttgac cttcaatgga cttaccctgg agaagtgaaa ggcaaaggca 841 tcacaatgct ggaagaaatc aaagtcccat ccatcaaatt ggtgtacact ttgacggtcc 901 ccgaggccac ggtgaaagac agtggagatt acgaatgtgc tgcccgccag gctaccaggg 961 aggtcaaaga aatgaagaaa gtcactattt ctgtccatga gaaaggtttc attgaaatca 1021 aacccacctt cagccagttg gaagctgtca acctgcatga agtcaaacat tttgttgtag 1081 aggtgcgggc ctacccacct cccaggatat cctggctgaa aaacaatctg actctgattg 1141 aaaatctcac tgagatcacc actgatgtgg aaaagattca ggaaataagg tatcgaagca 1201 aattaaagct gatccgtgct aaggaagaag acagtggcca ttatactatt gtagctcaaa 1261 atgaagatgc tgtgaagagc tatacttttg aactgttaac tcaagttcct tcatccattc 1321 tggacttggt cgatgatcac catggctcaa ctgggggaca gacggtgagg tgcacagctg 1381 aaggcacgcc gcttcctgat attgagtgga tgatatgcaa agatattaag aaatgtaata 1441 atgaaacttc ctggactatt ttggccaaca atgtctcaaa catcatcacg gagatccact 1501 cccgagacag gagtaccgtg gagggccgtg tgactttcgc caaagtggag gagaccatcg 1561 ccgtgcgatg cctggctaag aatctccttg gagctgagaa ccgagagctg aagctggtgg 1621 ctcccaccct gcgttctgaa ctcacggtgg ctgctgcagt cctggtgctg ttggtgattg 1681 tgatcatctc acttattgtc ctggttgtca tttggaaaca gaaaccgagg tatgaaattc 1741 gctggagggt cattgaatca atcagcccag atggacatga atatatttat gtggacccga 1801 tgcagctgcc ttatgactca agatgggagt ttccaagaga tggactagtg cttggtcggg 1861 tcttggggtc tggagcgttt gggaaggtgg ttgaaggaac agcctatgga ttaagccggt 1921 cccaacctgt catgaaagtt gcagtgaaga tgctaaaacc cacggccaga tccagtgaaa 1981 aacaagctct catgtctgaa ctgaagataa tgactcacct ggggccacat ttgaacattg 2041 taaacttgct gggagcctgc accaagtcag gccccattta catcatcaca gagtattgct 2101 tctatggaga tttggtcaac tatttgcata agaataggga tagcttcctg agccaccacc 2161 cagagaagcc aaagaaagag ctggatatct ttggattgaa ccctgctgat gaaagcacac 2221 ggagctatgt tattttatct tttgaaaaca atggtgacta catggacatg aagcaggctg 2281 atactacaca gtatgtcccc atgctagaaa ggaaagaggt ttctaaatat tccgacatcc 2341 agagatcact ctatgatcgt ccagcctcat ataagaagaa atctatgtta gactcagaag 2401 tcaaaaacct cctttcagat gataactcag aaggccttac tttattggat ttgttgagct 2461 tcacctatca agttgcccga ggaatggagt ttttggcttc aaaaaattgt gtccaccgtg 2521 atctggctgc tcgcaacgtc ctcctggcac aaggaaaaat tgtgaagatc tgtgactttg 2581 gcctggccag agacatcatg catgattcga actatgtgtc gaaaggcagt acctttctgc 2641 ccgtgaagtg gatggctcct gagagcatct ttgacaacct ctacaccaca ctgagtgatg 2701 tctggtctta tggcattctg ctctgggaga tcttttccct tggtggcacc ccttaccccg 2761 gcatgatggt ggattctact ttctacaata agatcaagag tgggtaccgg atggccaagc 2821 ctgaccacgc taccagtgaa gtctacgaga tcatggtgaa atgctggaac agtgagccgg 2881 agaagagacc ctccttttac caccTGAGTG AGATTGTGGA GAATCTGCTG CCTGGACAAT 2941 ATAAAAAGAG TTATGAAAAA ATTCACCTGG ACTTCCTGAA GAGTGACCAT CCTGCTGTGG 3001 CACGCATGCG TGTGGACTCA GACAATGCAT ACATTGGTGT CACCTACAAA AACGAGGAAG 3061 ACAAGCTGAA GGACTGGGAG GGTGGTCTGG ATGAGCAGAG ACTGAGCGCT GACAGTGGCT 3121 ACATCATTCC TCTGCCTGAC ATTGACCCTG TCCCTGAGGA GGAGGACCTG GGCAAGAGGA 3181 ACAGACACAG CTCGCAGACC TCTGAAGAGA GTGCCATTGA GACGGGTTCC AGCAGTTCCA 3241 CCTTCATCAA GAGAGAGGAC GAGACCATTG AAGACATCGA CATGATGGAC GACATCGGCA 3301 TAGACTCTTC AGACCTGGTG GAAGACAGCT TCCTGCCAAC TTTCTTGTAC AAAGTGGTTG 3361 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 3421 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 3481 CTCGTGGCCG GGGGTTCCCT GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 3541 ttgtgaaaga tt