Construct: ORF TRCN0000472946
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017413.1_s317c1
- Derived from:
- ccsbBroadEn_04147
- DNA Barcode:
- CTCATCCCTGCACTGGGAGCTTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PYROXD1 (79912)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472946
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79912 | PYROXD1 | pyridine nucleotide-disulph... | NM_024854.5 | 100% | 100% | |
| 2 | human | 79912 | PYROXD1 | pyridine nucleotide-disulph... | NM_001350912.1 | 85.8% | 85.8% | 0_1ins213 |
| 3 | human | 79912 | PYROXD1 | pyridine nucleotide-disulph... | XM_006719153.3 | 51% | 50.2% | (many diffs) |
| 4 | human | 79912 | PYROXD1 | pyridine nucleotide-disulph... | XM_017019976.2 | 50.3% | 50% | 0_1ins737;2T>G;4_5insACAAAAG |
| 5 | human | 79912 | PYROXD1 | pyridine nucleotide-disulph... | NM_001350913.2 | 48.2% | 48.2% | 0_1ins777 |
| 6 | human | 79912 | PYROXD1 | pyridine nucleotide-disulph... | XR_242902.4 | 36.5% | 1_101del;1095_1099delGTAAG;1607_4105del | |
| 7 | mouse | 232491 | Pyroxd1 | pyridine nucleotide-disulph... | NM_183165.3 | 82.8% | 84.8% | (many diffs) |
| 8 | mouse | 232491 | Pyroxd1 | pyridine nucleotide-disulph... | XM_006506954.3 | 71.7% | 73.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1566
- ORF length:
- 1500
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggcagcgcgc cctcccccga cggcagggaa gttcgtggtg gtcggcggcg 121 gcatcgcggg cgtcacttgt gcggagcagt tggctactca ctttccatcg gaagatattc 181 tcttggtaac agcttctcct gttattaaag cagttacaaa tttcaagcag atttctaaaa 241 tattggaaga attcgatgtt gaagaacaat caagtaccat gttaggaaaa cgctttccca 301 acattaaggt tatagaatct ggcgtaaagc aactgaagag tgaagaacac tgcattgtaa 361 cagaagatgg caatcagcac gtatataaga aactctgtct gtgtgctgga gctaaaccaa 421 agttgatatg tgaaggaaat ccttatgtat taggaatccg tgatacagac agtgctcagg 481 aatttcagaa acagcttact aaagctaaaa gaataatgat catagggaac ggtggtattg 541 cacttgagtt agtgtatgaa attgaaggct gtgaagtgat ttgggccatt aaagataaag 601 ctatagggaa tactttcttc gatgcaggag cagctgaatt cttgacttca aagctcattg 661 ctgaaaaatc agaggctaaa attgcacata aaagaaccag atatacaact gaaggaagga 721 aaaaggaagc tagaagcaaa tctaaagcag ataatgtagg aagtgcattg ggaccagatt 781 ggcatgaagg cttgaatctt aaaggaacaa aagagttttc tcataagatt caccttgaaa 841 ctatgtgtga agtaaagaaa atctaccttc aggatgagtt tagaattttg aagaaaaagt 901 ccttcacttt tccaagagac cataagtcag ttacagctga tacagagatg tggcctgtct 961 atgtggaatt gaccaatgaa aagatatatg gctgcgattt cattgtcagt gctacaggag 1021 ttacaccaaa tgtagaacct tttctccatg gtaacagttt tgatctagga gaagatggtg 1081 gcctgaaagt ggatgatcat atgcacacat cccttcctga tatctatgct gccggtgaca 1141 tctgtactac atcctggcag ctgagcccag tctggCAGCA GATGAGGCTG TGGACCCAGG 1201 CTAGACAGAT GGGATGGTAT GCAGCAAAGT GCATGGCTGC AGCGAGTTCA GGAGACTCTA 1261 TTGACATGGA TTTCAGCTTT GAACTGTTTG CTCATGTGAC AAAATTTTTT AACTATAAGG 1321 TTGTACTGCT GGGAAAATAC AATGCACAGG GCTTAGGTTC AGATCATGAA TTAATGCTGA 1381 GATGTACCAA AGGACGAGAA TACATCAAAG TCGTCATGCA AAATGGACGA ATGATGGGAG 1441 CTGTCTTAAT TGGTGAAACC GATTTAGAAG AAACATTTGA AAACCTAATC TTAAACCAAA 1501 TGAATCTTTC ATCATATGGA GAAGATCTGC TAGATCCAAA TATTGATATA GAAGATTATT 1561 TTGACTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1621 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1681 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTCATCCCT GCACTGGGAG CTTCCACGCG 1741 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt