Construct: ORF TRCN0000473009
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004804.1_s317c1
- Derived from:
- ccsbBroadEn_00043
- DNA Barcode:
- AACGTATCCTATGGGACCTCAGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AIF1 (199)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473009
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 199 | AIF1 | allograft inflammatory fact... | NM_001623.5 | 100% | 100% | |
| 2 | human | 199 | AIF1 | allograft inflammatory fact... | NM_001318970.2 | 63.2% | 63.2% | 0_1ins162 |
| 3 | human | 199 | AIF1 | allograft inflammatory fact... | NM_032955.3 | 63.2% | 63.2% | 0_1ins162 |
| 4 | human | 199 | AIF1 | allograft inflammatory fact... | XM_005248870.4 | 56.3% | 56.3% | 196_393del;558_559ins81 |
| 5 | human | 199 | AIF1 | allograft inflammatory fact... | XM_017010332.1 | 48.9% | 30.9% | (many diffs) |
| 6 | mouse | 11629 | Aif1 | allograft inflammatory fact... | NM_019467.2 | 89.1% | 89.1% | (many diffs) |
| 7 | mouse | 11629 | Aif1 | allograft inflammatory fact... | XM_006523503.3 | 89.1% | 89.1% | (many diffs) |
| 8 | mouse | 11629 | Aif1 | allograft inflammatory fact... | XM_006523504.3 | 89.1% | 89.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 531
- ORF length:
- 441
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAGCCAAAC CAGGGATTTA CAGGGAGGAA 121 AAGCTTTCGG ACTGCTGAAG GCCCAGCAGG AAGAGAGGCT GGATGAGATC AACAAGCAAT 181 TCCTAGACGA TCCCAAATAT AGCAGTGATG AGGATCTGCC CTCCAAACTG GAAGGCTTCA 241 AAGAGAAATA CATGGAGTTT GACCTTAATG GAAATGGCGA TATTGATATC ATGTCCCTGA 301 AACGAATGCT GGAGAAACTT GGAGTCCCCA AGACTCACCT AGAGCTAAAG AAATTAATTG 361 GAGAGGTGTC CAGTGGCTCC GGGGAGACGT TCAGCTACCC TGACTTTCTC AGGATGATGC 421 TGGGCAAGAG ATCTGCCATC CTAAAAATGA TCCTGATGTA TGAGGAAAAA GCGAGAGAAA 481 AGGAAAAGCC AACAGGCCCC CCAGCCAAGA AAGCTATCTC TGAGTTGCCC TGGCCCACTT 541 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 601 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 661 CTTGTGGAAA GGACGAAACG TATCCTATGG GACCTCAGTG ACGCGTTAAG TCgacaatca 721 acctctggat tacaaaattt gtgaaagatt