Construct: ORF TRCN0000473064

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018159.4_s317c1
Derived from:
ccsbBroadEn_14941
DNA Barcode:
CATTCACGTCCACACCCTGCCGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SH3BP5 (9467)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473064
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9467 SH3BP5 SH3 domain binding protein 5 XM_011534251.2 96.7% 96.7% (many diffs)
2 human 9467 SH3BP5 SH3 domain binding protein 5 XM_017007521.2 94.3% 93.2% (many diffs)
3 human 9467 SH3BP5 SH3 domain binding protein 5 NM_004844.5 93.4% 93.4% 1_90del
4 human 9467 SH3BP5 SH3 domain binding protein 5 NM_001018009.4 70.1% 70.1% 0_1ins381
5 human 9467 SH3BP5 SH3 domain binding protein 5 XM_017007522.2 70.1% 70.1% 0_1ins381
6 human 9467 SH3BP5 SH3 domain binding protein 5 XM_017007523.2 70.1% 70.1% 0_1ins381
7 human 9467 SH3BP5 SH3 domain binding protein 5 XM_017007524.1 70.1% 70.1% 0_1ins381
8 human 9467 SH3BP5 SH3 domain binding protein 5 XM_017007525.1 70.1% 70.1% 0_1ins381
9 mouse 24056 Sh3bp5 SH3-domain binding protein ... NM_011894.3 82.6% 87.1% (many diffs)
10 mouse 24056 Sh3bp5 SH3-domain binding protein ... NM_001347585.1 79.2% 77.7% (many diffs)
11 mouse 24056 Sh3bp5 SH3-domain binding protein ... XM_006519015.2 62.2% 64.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1341
ORF length:
1275
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gcaggggctg gaggaggaag aagaggtgga tccccggatc cagggagaac
121tggagaagtt aaatcagtcc acggatgata tcaacagacg ggagactgaa cttgaggatg
181ctcgtcagaa gttccgctct gttctggttg aagcaacggt gaaactggat gaactggtga
241agaaaattgg caaagctgtg gaagactcca agccctactg ggaggcacgg agggtggcga
301ggcaggctca gctggaagct cagaaagcca cgcaggactt ccagagggcc acagaggtgc
361tccgtgccgc caaggagacc atctccctgg ccgagcagcg gctgctggag gatgacaagc
421ggcagttcga ctccgcctgg caggagatgc tgaatcacgc cactcagagg gtcatggagg
481cggagcagac caagaccagg agcgagctgg tgcataagga gacggcagcc aggtacaatg
541ccgccatggg ccgcatgcga cagctggaga agaaactcaa gagagccatc aacaagtcca
601agccttattt tgaactcaag gcaaagtact atgtgcagct cgagcaactg aaaaagactg
661tggatgacct gcaggccaaa ctgaccctgg caaaaggcga gtacaagatg gccctgaaga
721acctggagat gatctcagat gagatccacg agcggcggcg ctccagtgcc atggggcctc
781ggggatgcgg tgttggtgct gagggcagca gcacatctgt ggaggatctg ccagggagca
841aacctgagcc tgatgccatt tctgtggcct cggaggcctt tgaagatgac agctgtagca
901actttgtgtc tgaagatgac tcggaaaccc agtccgtgtc cagctttagt tcaggaccaa
961caagcccgtc tgagatgcct gaccagttcc cTGCGGTTGT GAGGCCTGGC AGCCTGGATC
1021TGCCCAGCCC TGTGTCCCTG TCAGAGTTTG GGATGATGTT CCCAGTGTTG GGCCCTCGAA
1081GTGAATGCAG CGGGGCCTCC TCCCCTGAAT GTGAAGTAGA ACGAGGAGAC AGGGCAGAAG
1141GGGCAGAGAA TAAAACAAGT GACAAAGCCA ACAACAACCG GGGCCTCAGC AGTAGCAGTG
1201GCAGTGGTGG CAGCAGTAAG AGCCAAAGCA GCACCTCCCC TGAGGGCCAG GCCTTGGAGA
1261ACCGGATGAA GCAGCTCTCC CTACAGTGCT CAAAGGGAAG AGATGGAATT ATTGCTGACA
1321TAAAAATGGT GCAGATTGGC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1381CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1441AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACATT CACGTCCACA
1501CCCTGCCGCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)