Construct: ORF TRCN0000473127
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001222.1_s317c1
- Derived from:
- ccsbBroadEn_07194
- DNA Barcode:
- ACCTTGACTCCTCTATATGGAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLRA3 (8001)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473127
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8001 | GLRA3 | glycine receptor alpha 3 | NM_006529.4 | 99.7% | 100% | 822A>G;876T>C;1080T>C |
| 2 | human | 8001 | GLRA3 | glycine receptor alpha 3 | NM_001042543.3 | 96.6% | 96.7% | 822A>G;876T>C;1070_1071ins45 |
| 3 | human | 8001 | GLRA3 | glycine receptor alpha 3 | XM_017008630.1 | 91.1% | 91.3% | (many diffs) |
| 4 | human | 8001 | GLRA3 | glycine receptor alpha 3 | XM_011532267.2 | 66.5% | 66.5% | (many diffs) |
| 5 | human | 8001 | GLRA3 | glycine receptor alpha 3 | XM_011532268.2 | 57.7% | 57.9% | (many diffs) |
| 6 | human | 8001 | GLRA3 | glycine receptor alpha 3 | XR_001741328.1 | 52% | (many diffs) | |
| 7 | human | 8001 | GLRA3 | glycine receptor alpha 3 | XR_001741329.1 | 50% | (many diffs) | |
| 8 | mouse | 110304 | Glra3 | glycine receptor, alpha 3 s... | NM_080438.2 | 86.2% | 95.6% | (many diffs) |
| 9 | mouse | 110304 | Glra3 | glycine receptor, alpha 3 s... | XM_006509247.2 | 83.4% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1458
- ORF length:
- 1392
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccacgtgaga cactttcgga cattagtttc gggattttac ttctgggaag 121 cagcactgtt actcagtttg gttgccacaa aggaaacaga cagtgcaaga tctcgaagtg 181 ctccaatgtc accttctgat tttctggata aattaatggg caggacatca ggatatgatg 241 caagaatcag acccaatttt aaaggccctc cagttaatgt cacatgcaac atattcatca 301 acagctttgg ctctatcgca gagacgacca tggattacag agtgaatatc tttcttcgtc 361 agaaatggaa tgatccccgc ctcgcgtaca gtgaatatcc tgacgactct ttagacctcg 421 acccctccat gttggactcc atttggaaac ctgatttgtt ctttgccaat gaaaagggtg 481 ccaactttca tgaagtcact acagacaaca aattgctaag aattttcaaa aatggaaatg 541 ttctttattc aataagatta acattaacac tttcctgtcc aatggatctc aagaattttc 601 ccatggatgt acaaacatgt ataatgcaac tggaaagctt tgggtacaca atgaatgatc 661 tcatttttga atggcaagat gaggcacccg tacaagtggc agaaggactc actttgcccc 721 agtttctgtt gaaagaagaa aaagatttac gatactgcac taaacattac aatacaggaa 781 agtttacgtg tatagaagtg cgattccatc tggagcgaca aatgggatac tatctgatcc 841 agatgtacat tcccagtctc ctgattgtta ttctatcctg ggtttcgttc tggatcaaca 901 tggatgcagc accggccagg gtagctctgg ggataaccac cgtgctaacg atgactacac 961 agagttcagg atcacgagct tccttgccaa aagtttcata tgtcaaagct attgatattt 1021 ggatggcagt atgcctcctt tttgtgtttt cagcacttct GGAGTATGCA GCTGTAAATT 1081 TTGTATCAAG ACAACACAAA GAACTTCTGA GATTTCGACG AAAGAGAAAG AATAAGACAG 1141 AAGCCTTTGC ACTGGAGAAG TTTTACCGTT TCTCAGATAT GGATGATGAG GTAAGGGAAA 1201 GCCGATTCAG CTTCACAGCC TATGGAATGG GACCATGTCT ACAAGCAAAG GATGGCATGA 1261 CTCCAAAGGG CCCCAACCAC CCTGTCCAGG TAATGCCAAA AAGTCCTGAT GAAATGAGGA 1321 AGGTCTTTAT CGACCGGGCC AAGAAGATTG ATACCATCTC CCGAGCCTGC TTCCCATTAG 1381 CTTTTTTGAT TTTTAATATT TTCTACTGGG TTATCTATAA AATTCTTAGG CATGAGGATA 1441 TTCATCAGCA GCAAGATTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1501 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1561 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACCTTGA CTCCTCTATA 1621 TGGAAATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt