Construct: ORF TRCN0000473328
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006636.1_s317c1
- Derived from:
- ccsbBroadEn_06185
- DNA Barcode:
- TCGCTGCCATAGAGTGCTCGAAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EYA1 (2138)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473328
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_000503.6 | 99.9% | 100% | 813A>G |
| 2 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_001370334.1 | 99.9% | 100% | 813A>G |
| 3 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_001370335.1 | 99.9% | 100% | 813A>G |
| 4 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_172058.4 | 99.9% | 100% | 813A>G |
| 5 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_011517483.2 | 99.7% | 99.6% | 272_273insCAG;810A>G |
| 6 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013208.2 | 99.7% | 99.6% | 272_273insCAG;810A>G |
| 7 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_001288574.2 | 98.9% | 98.8% | 272_273insCAG;414_415insGGTGCATTGTGGGCA;795A>G |
| 8 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013211.2 | 95.2% | 91.6% | (many diffs) |
| 9 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_001370333.1 | 95.2% | 95.3% | 1_87del;900A>G |
| 10 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013202.1 | 95.2% | 95.3% | 1_87del;900A>G |
| 11 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013203.2 | 95.1% | 95% | 1_87del;359_360insCAG;897A>G |
| 12 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_011517484.3 | 94.4% | 94.5% | 1_87del;504_505insGGTGCATTGTGGGCA;885A>G |
| 13 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013204.2 | 94.3% | 94.2% | (many diffs) |
| 14 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013212.2 | 93.8% | 93.2% | (many diffs) |
| 15 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013205.2 | 91.8% | 91.4% | (many diffs) |
| 16 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013207.2 | 90.4% | 90.4% | 1_87del;900A>G;1137_1138ins90 |
| 17 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_172059.5 | 89.6% | 89.6% | (many diffs) |
| 18 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_001370336.1 | 89.4% | 89.3% | (many diffs) |
| 19 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013213.1 | 80.1% | 80.2% | 0_1ins351;462A>G |
| 20 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_001288575.2 | 79.3% | 79.3% | 0_1ins351;66_67insGGTGCATTGTGGGCA;447A>G |
| 21 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_011238344.2 | 90.5% | 96.4% | (many diffs) |
| 22 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | NM_001310459.1 | 90.4% | 96.1% | (many diffs) |
| 23 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_017315348.1 | 90.4% | 96.1% | (many diffs) |
| 24 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | NM_010164.2 | 89.7% | 95.6% | (many diffs) |
| 25 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_006495451.3 | 89.5% | 95.2% | (many diffs) |
| 26 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_006495446.3 | 88% | 88.3% | (many diffs) |
| 27 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | NM_001252192.1 | 84.7% | 89.8% | (many diffs) |
| 28 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_006495452.3 | 82.5% | 82.3% | (many diffs) |
| 29 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_011238346.2 | 82.5% | 82.3% | (many diffs) |
| 30 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_017315381.1 | 82.5% | 82.3% | (many diffs) |
| 31 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_011238347.2 | 75.5% | 79.7% | (many diffs) |
| 32 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_017315414.1 | 72.2% | 78.5% | (many diffs) |
| 33 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_017315422.1 | 72.2% | 78.5% | (many diffs) |
| 34 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XR_001780756.1 | 57.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1845
- ORF length:
- 1776
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaatgcag gatctaacca gcccgcatag ccgtctgagt ggtagtagtg 121 aatcccccag tggccccaaa ctcggtaact ctcatataaa tagtaattcc atgactccca 181 atggcaccga agttaaaaca gagccaatga gcagcagtga aacagcttca acgacagccg 241 acgggtcttt aaacaatttc tcaggttcag caattgggag cagtagtttc agcccacgac 301 caactcacca gttctctcca ccacagattt acccttccaa cagaccatac ccacatattc 361 tccctacccc ttcctcacaa actatggctg catatgggca aacacagttt accacaggaa 421 tgcaacaagc tacagcctat gccacgtacc cacagccagg acagccgtac ggcatttcct 481 catatggtgc attgtgggca ggcatcaaga ctgaaggtgg attgtcacag tctcagtcac 541 ctggacagac aggatttctc agctatggca caagcttcag tacccctcaa cctggacagg 601 caccatacag ctaccagatg caaggtagca gttttacaac atcatcagga atatatacag 661 gaaataattc actcacaaat tcctctggat ttaatagttc acagcaggac tatccgtctt 721 atcccagttt tggccagggt cagtacgcac agtattataa cagctcaccg tatccagcac 781 attatatgac cagcagcaac accagcccaa cgacaccatc caccaatgcc acttaccagc 841 ttcaagaacc gccatctggc atcaccagcc aagcagttac ggatcccaca gcagagtaca 901 gcacaatcca cagcccatca acacccatta aagattcaga ttctgatcga ttgcgtcgag 961 gttcagatgg gaaatcacgt ggacggggcc gaagaaacaa taatccttca cctcccccag 1021 attctgatct tgagagagtg ttcatctggg acttggatga gacaatcatt gttttccact 1081 ccttgcttac tgggtcctac gccaacagat atgggaggga tccacccact tcagtttccc 1141 ttggactgcg aatggaagaa atgattttca acttggcaga cacacattta ttttttaatg 1201 acttagaaga atgtgaccaa gtccatatag atgatgtttc ttcagatgat aacggacagg 1261 acctaagcac atataacttt ggaacagatg gctttcctgc tgcagcaacc agtgctaact 1321 tatgtttggc aactggtgta cggggcggtg tggactggat gagaaagttg gccttccgct 1381 acagacgggt aaaagagatc tacaacacct acaaaaataa tgttGGAGGT CTGCTTGGTC 1441 CAGCTAAGAG GGAAGCCTGG CTGCAGTTGA GGGCCGAAAT TGAAGCCCTG ACCGACTCCT 1501 GGTTGACACT GGCCCTGAAA GCACTCTCGC TCATTCACTC CCGGACAAAC TGTGTGAATA 1561 TTTTAGTAAC AACTACTCAG CTCATCCCAG CATTGGCGAA AGTCCTGCTG TATGGGTTAG 1621 GAATTGTATT TCCAATAGAA AATATTTACA GTGCAACTAA AATAGGAAAA GAAAGCTGTT 1681 TTGAGAGAAT AATTCAAAGG TTTGGAAGAA AAGTGGTGTA TGTTGTTATA GGAGATGGTG 1741 TAGAAGAAGA ACAAGGAGCA AAAAAGCACG CGATGCCCTT CTGGAGGATC TCCAGCCACT 1801 CGGACCTCAT GGCCCTGCAC CATGCCTTGG AACTGGAGTA CCTGTTGCCA ACTTTCTTGT 1861 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1921 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1981 GAAAGGACGA TCGCTGCCAT AGAGTGCTCG AAGAACGCGT TAAGTCgaca atcaacctct 2041 ggattacaaa atttgtgaaa gatt