Construct: ORF TRCN0000473334
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015231.1_s317c1
- Derived from:
- ccsbBroadEn_05520
- DNA Barcode:
- TCTCCACCCTGTCCCCAATCTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LCE1B (353132)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473334
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 353132 | LCE1B | late cornified envelope 1B | NM_178349.2 | 100% | 100% | |
| 2 | human | 353133 | LCE1C | late cornified envelope 1C | NM_178351.4 | 96% | 96.6% | (many diffs) |
| 3 | human | 353135 | LCE1E | late cornified envelope 1E | NM_178353.2 | 93.7% | 93.2% | (many diffs) |
| 4 | human | 353137 | LCE1F | late cornified envelope 1F | NM_178354.2 | 89.4% | 90.6% | (many diffs) |
| 5 | human | 353134 | LCE1D | late cornified envelope 1D | NM_178352.3 | 88.9% | 86.5% | (many diffs) |
| 6 | human | 353131 | LCE1A | late cornified envelope 1A | NM_178348.2 | 87.2% | 85.5% | (many diffs) |
| 7 | human | 353133 | LCE1C | late cornified envelope 1C | NM_001276331.2 | 72.8% | 72.8% | (many diffs) |
| 8 | human | 199834 | LCE4A | late cornified envelope 4A | NM_178356.3 | 62.5% | 60.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 447
- ORF length:
- 354
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGTCCTG CCAGCAGAAC CAGCAGCAGT 121 GCCAGCCCCC TCCCAAGTGC ATCCCCAAGT GCCCTCCCAA GTGCCTCACC CCTAGATGCC 181 CCCCAAAGTG TCCCCCTAAG TGTCCTCCAG TCTCTTCCTG CTGCAGTGTC AGCTCCGGAG 241 GCTGCTGTGG CTCCAGCTCT GGGGGAAGCT GTGGCTCCAG CTCTGGGGGT TGCTGCAGTT 301 CTGGGGGAGG TGGCTGCTGC CTGAGCCACC ACAGGCGCCG TAGGTCCCAC TGCCACAGAC 361 CCCAGAGCTC TGGCTGCTGC AGCCAGCCCT CCGGGGGCTC CAGCTGCTGT GGAGGAGGGA 421 GTGGCCAGCA CTCTGGAGGC TGCTGCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 481 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 541 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCTCCACC 601 CTGTCCCCAA TCTTTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 661 aagatt