Construct: ORF TRCN0000473396
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005870.1_s317c1
- Derived from:
- ccsbBroadEn_09934
- DNA Barcode:
- TTTGTCACGATCCCTGTGCTCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MKX (283078)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473396
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283078 | MKX | mohawk homeobox | NM_001242702.2 | 99.7% | 99.7% | 31_32delGGinsTC;852C>T |
| 2 | human | 283078 | MKX | mohawk homeobox | NM_173576.3 | 99.7% | 99.7% | 31_32delGGinsTC;852C>T |
| 3 | human | 283078 | MKX | mohawk homeobox | XM_017016105.1 | 99.7% | 99.7% | 31_32delGGinsTC;852C>T |
| 4 | human | 283078 | MKX | mohawk homeobox | XM_017016106.1 | 99.7% | 99.7% | 31_32delGGinsTC;852C>T |
| 5 | human | 283078 | MKX | mohawk homeobox | XM_017016108.1 | 82.3% | 82.3% | 31_32delGGinsTC;852C>T;873_874ins183 |
| 6 | human | 283078 | MKX | mohawk homeobox | XM_017016107.1 | 81.9% | 80.4% | (many diffs) |
| 7 | mouse | 210719 | Mkx | mohawk homeobox | NM_177595.4 | 84.7% | 88.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1122
- ORF length:
- 1056
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa caccatcgtc ttcaacaagc tcagctctgc ggtgctgttt gaggacggag 121 gcgcctcgga gcgggagcgg ggtggccggc cctacagcgg tgtcctggac agtcctcacg 181 cccgccccga ggtgggcatt cccgacggcc cgcccctcaa ggacaacctc ggcctgagac 241 accggaggac cggcgcccgg cagaatggcg ggaaggtgag gcacaagcgg caggccctgc 301 aagacatggc gcgacccctc aagcagtggc tttacaagca ccgtgacaac ccgtacccca 361 ccaagaccga gaagatactc ttggccctcg gctcgcagat gacgctagtg caggtgtcaa 421 attggtttgc taatgcaaga cgtcggctta agaataccgt tcgacagcca gatttaagct 481 gggctttgag aataaagtta tacaacaagt atgttcaagg caatgctgaa cggcttagcg 541 taagcagtga tgactcatgt tctgaagatg gagaaaatcc tccaagaacc cacatgaacg 601 aagggggcta taatacccca gttcaccatc ctgtgattaa aagtgagaat tcggtcatca 661 aagcgggagt gaggccagag tcacgggcca gtgaggacta cgtggcaccc cccaaataca 721 agagcagctt gttgaaccgt taccTTAATG ACTCTTTGAG ACATGTCATG GCCACGAACA 781 CTACCATGAT GGGAAAAACA AGGCAAAGAA ACCACTCGGG ATCTTTTAGC TCCAATGAAT 841 TTGAGGAAGA ATTAGTGTCT CCATCGTCAT CAGAAACTGA AGGCAACTTT GTCTATCGCA 901 CAGACACTCT GGAAAATGGA TCCAATAAGG GTGAAAGCGC AGCTAACAGA AAAGGACCAA 961 GCAAGGATGA CACGTATTGG AAGGAGATCA ACGCAGCTAT GGCCTTAACA AATCTTGCAC 1021 AGGGAAAGGA CAAACTGCAG GGAACTACCA GCTGCATCAT CCAGAAGTCG TCCCATATAG 1081 CAGAAGTAAA GACTGTCAAA GTGCCGCTGG TGCAGCAGTT TTACCCAACT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGATTT GTCACGATCC CTGTGCTCGG GACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t