Construct: ORF TRCN0000473406
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018994.2_s317c1
- Derived from:
- ccsbBroadEn_14671
- DNA Barcode:
- AAAATCAGCCCATACACAGGGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGF1R (3480)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473406
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3480 | IGF1R | insulin like growth factor ... | NM_000875.5 | 99.9% | 100% | 3984C>T |
| 2 | human | 3480 | IGF1R | insulin like growth factor ... | NM_001291858.2 | 99.9% | 99.8% | 2782_2783insCAG;3981C>T |
| 3 | human | 3480 | IGF1R | insulin like growth factor ... | XM_017022136.1 | 97.2% | 96.4% | (many diffs) |
| 4 | human | 3480 | IGF1R | insulin like growth factor ... | XM_017022137.1 | 97.2% | 96.4% | (many diffs) |
| 5 | human | 3480 | IGF1R | insulin like growth factor ... | XM_017022138.1 | 97.1% | 96.3% | (many diffs) |
| 6 | human | 3480 | IGF1R | insulin like growth factor ... | XM_017022139.1 | 91.1% | 91.1% | 0_1ins363;3621C>T |
| 7 | human | 3480 | IGF1R | insulin like growth factor ... | XM_011521516.2 | 77.8% | 77.8% | 0_1ins909;3075C>T |
| 8 | human | 3480 | IGF1R | insulin like growth factor ... | XM_024449913.1 | 77.8% | 77.8% | 0_1ins909;3075C>T |
| 9 | human | 3480 | IGF1R | insulin like growth factor ... | XM_011521517.2 | 67.4% | 67.4% | 0_1ins1335;2649C>T |
| 10 | mouse | 16001 | Igf1r | insulin-like growth factor ... | NM_010513.2 | 89.4% | 95.9% | (many diffs) |
| 11 | mouse | 16001 | Igf1r | insulin-like growth factor ... | XM_006540643.3 | 89.3% | 95.9% | (many diffs) |
| 12 | mouse | 16001 | Igf1r | insulin-like growth factor ... | XM_006540642.3 | 87.4% | 93.8% | (many diffs) |
| 13 | mouse | 16001 | Igf1r | insulin-like growth factor ... | XM_006540641.3 | 87.3% | 93.7% | (many diffs) |
| 14 | mouse | 16001 | Igf1r | insulin-like growth factor ... | XM_006540645.3 | 75.2% | 80% | (many diffs) |
| 15 | mouse | 16001 | Igf1r | insulin-like growth factor ... | XM_006540644.3 | 73.5% | 78.2% | (many diffs) |
| 16 | mouse | 16001 | Igf1r | insulin-like growth factor ... | XM_017321986.1 | 67.6% | 73.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4170
- ORF length:
- 4101
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagtctggc tccggaggag ggtccccgac ctcgctgtgg gggctcctgt 121 ttctctccgc cgcgctctcg ctctggccga cgagtggaga aatctgcggg ccaggcatcg 181 acatccgcaa cgactatcag cagctgaagc gcctggagaa ctgcacggtg atcgagggct 241 acctccacat cctgctcatc tccaaggccg aggactaccg cagctaccgc ttccccaagc 301 tcacggtcat taccgagtac ttgctgctgt tccgagtggc tggcctcgag agcctcggag 361 acctcttccc caacctcacg gtcatccgcg gctggaaact cttctacaac tacgccctgg 421 tcatcttcga gatgaccaat ctcaaggata ttgggcttta caacctgagg aacattactc 481 ggggggccat caggattgag aaaaatgctg acctctgtta cctctccact gtggactggt 541 ccctgatcct ggatgcggtg tccaataact acattgtggg gaataagccc ccaaaggaat 601 gtggggacct gtgtccaggg accatggagg agaagccgat gtgtgagaag accaccatca 661 acaatgagta caactaccgc tgctggacca caaaccgctg ccagaaaatg tgcccaagca 721 cgtgtgggaa gcgggcgtgc accgagaaca atgagtgctg ccaccccgag tgcctgggca 781 gctgcagcgc gcctgacaac gacacggcct gtgtagcttg ccgccactac tactatgccg 841 gtgtctgtgt gcctgcctgc ccgcccaaca cctacaggtt tgagggctgg cgctgtgtgg 901 accgtgactt ctgcgccaac atcctcagcg ccgagagcag cgactccgag gggtttgtga 961 tccacgacgg cgagtgcatg caggagtgcc cctcgggctt catccgcaac ggcagccaga 1021 gcatgtactg catcccttgt gaaggtcctt gcccgaaggt ctgtgaggaa gaaaagaaaa 1081 caaagaccat tgattctgtt acttctgctc agatgctcca aggatgcacc atcttcaagg 1141 gcaatttgct cattaacatc cgacggggga ataacattgc ttcagagctg gagaacttca 1201 tggggctcat cgaggtggtg acgggctacg tgaagatccg ccattctcat gccttggtct 1261 ccttgtcctt cctaaaaaac cttcgcctca tcctaggaga ggagcagcta gaagggaatt 1321 actccttcta cgtcctcgac aaccagaact tgcagcaact gtgggactgg gaccaccgca 1381 acctgaccat caaagcaggg aaaatgtact ttgctttcaa tcccaaatta tgtgtttccg 1441 aaatttaccg catggaggaa gtgacgggga ctaaagggcg ccaaagcaaa ggggacataa 1501 acaccaggaa caacggggag agagcctcct gtgaaagtga cgtcctgcat ttcacctcca 1561 ccaccacgtc gaagaatcgc atcatcataa cctggcaccg gtaccggccc cctgactaca 1621 gggatctcat cagcttcacc gtttactaca aggaagcacc ctttaagaat gtcacagagt 1681 atgatgggca ggatgcctgc ggctccaaca gctggaacat ggtggacgtg gacctcccgc 1741 ccaacaagga cgtggagccc ggcatcttac tacatgggct gaagccctgg actcagtacg 1801 ccgtttacgt caaggctgtg accctcacca tggtggagaa cgaccatatc cgtggggcca 1861 agagtgagat cttgtacatt cgcaccaatg cttcagttcc ttccattccc ttggacgttc 1921 tttcagcatc gaactcctct tctcagttaa tcgtgaagtg gaaccctccc tctctgccca 1981 acggcaacct gagttactac attgtgcgct ggcagcggca gcctcaggac ggctaccttt 2041 accggcacaa ttactgctcc aaagacaaaa tccccatcag gaagtatgcc gacggcacca 2101 tcgacattga ggaggtcaca gagaacccca agactgaggt gtgtggtggg gagaaagggc 2161 cttgctgcgc ctgccccaaa actgaagccg agaagcaggc cgagaaggag gaggctgaat 2221 accgcaaagt ctttgagaat ttcctgcaca actccatctt cgtgcccaga cctgaaagga 2281 agcggagaga tgtcatgcaa gtggccaaca ccaccatgtc cagccgaagc aggaacacca 2341 cggccgcaga cacctacaac atcaccgacc cggaagagct ggagacagag taccctttct 2401 ttgagagcag agtggataac aaggagagaa ctgtcatttc taaccttcgg cctttcacat 2461 tgtaccgcat cgatatccac agctgcaacc acgaggctga gaagctgggc tgcagcgcct 2521 ccaacttcgt ctttgcaagg actatgcccg cagaaggagc agatgacatt cctgggccag 2581 tgacctggga gccaaggcct gaaaactcca tctttttaaa gtggccggaa cctgagaatc 2641 ccaatggatt gattctaatg tatgaaataa aatacggatc acaagttgag gatcagcgag 2701 aatgtgtgtc cagacaggaa tacaggaagt atggaggggc caagctaaac cggctaaacc 2761 cggggaacta cacagcccgg attcaggcca catctctctc tgggaatggg tcgtggacag 2821 atcctgtgtt cttctatgtc caggccaaaa caggatatga aaacttcatc catctgatca 2881 tcgctctgcc cgtcgctgtc ctgttgatcg tgggagggtt ggtgattatg ctgtacgtct 2941 tccatagaaa gagaaataac agcaggctgg ggaatggagt gctgtatgcc tctgtgaacc 3001 cggagtactt cagcgctgct gatgtgtacg ttcctgatga gtgggaggtg gctcgggaga 3061 agatcaccat gagccgggaa cttgggcagg ggtcgtttgg gatggtctat gaaggagttg 3121 ccaagggtgt ggtgaaagat gaacctgaaa ccagagtggc cattaaaaca gtgaacgagg 3181 ccgcaagcat gcgtgagagg attgagtttc tcaacgaagc ttctgtgatg aaggagttca 3241 attgtcacca tgtggtgcga ttgctgggtg tggtgtccca aggccagcca acactggtca 3301 tcatggaact gatgacacgg ggcgatctca aaagttatct ccggtctctg aggccagaaa 3361 tggagaataa tccagtccta gcacctccaa gcctgagcaa gatgattcag atggccggag 3421 agattgcaga cggcatggca tacctcaacg ccaataagtt cgtccacaga gaccttgctg 3481 cccggaattg catggtagcc gaagatttca cagtcaaaat cggagatttt ggtatgacgc 3541 gagatatcta tgagacagac tattaccgga aaggagggaa agggctgctg cccgtgcgct 3601 ggatgtctcc tgagtccctc aaggatggag tcttcaccac ttactcggac gtctggtcct 3661 tcggggtcgt cctctgggag atcgccacac tggccgagca gccctaccag ggcttgtcca 3721 acgagcaagt ccttcgcttc gtcatggagg gcggccTTCT GGACAAGCCA GACAACTGTC 3781 CTGACATGCT GTTTGAACTG ATGCGCATGT GCTGGCAGTA TAACCCCAAG ATGAGGCCTT 3841 CCTTCCTGGA GATCATCAGC AGCATCAAAG AGGAGATGGA GCCTGGCTTC CGGGAGGTCT 3901 CCTTCTACTA CAGCGAGGAG AACAAGCTGC CCGAGCCGGA GGAGCTGGAC CTGGAGCCAG 3961 AGAACATGGA GAGCGTCCCC CTGGACCCCT CGGCCTCCTC GTCCTCCCTG CCACTGCCCG 4021 ACAGACACTC AGGACACAAG GCCGAGAACG GTCCCGGCCC TGGGGTGCTG GTCCTCCGCG 4081 CCAGCTTCGA CGAGAGACAG CCTTACGCCC ACATGAACGG GGGCCGCAAG AACGAGCGGG 4141 CCTTGCCGCT GCCCCAGTCT TCGACCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 4201 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 4261 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAAATCAG 4321 CCCATACACA GGGTTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 4381 aagatt