Transcript: Human XM_011521516.2

PREDICTED: Homo sapiens insulin like growth factor 1 receptor (IGF1R), transcript variant X6, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
IGF1R (3480)
Length:
4681
CDS:
403..3597

Additional Resources:

NCBI RefSeq record:
XM_011521516.2
NBCI Gene record:
IGF1R (3480)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145186 GGAGAACGACCATATCCGTG pXPR_003 GGG 874 27% 7 0.7296 IGF1R IGF1R 76692
2 BRDN0001146028 GGTACAATGTGAAAGGCCGA pXPR_003 AGG 1472 46% 10 0.2009 IGF1R IGF1R 76689
3 BRDN0001146062 TTCCGAAATTTACCGCATGG pXPR_003 AGG 475 15% 5 -0.7783 IGF1R IGF1R 76691
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011521516.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000121196 GCTGTACGTCTTCCATAGAAA pLKO.1 2355 CDS 100% 5.625 7.875 N IGF1R n/a
2 TRCN0000039674 CGGCACAATTACTGCTCCAAA pLKO.1 1468 CDS 100% 4.950 6.930 N IGF1R n/a
3 TRCN0000000424 GCTGATGTGTACGTTCCTGAT pLKO.1 2443 CDS 100% 4.050 5.670 N IGF1R n/a
4 TRCN0000121297 CCTCTCTGCTTCATAACGGAA pLKO.1 3932 3UTR 100% 2.640 3.696 N IGF1R n/a
5 TRCN0000039677 GCCTTTCACATTGTACCGCAT pLKO.1 1875 CDS 100% 2.160 3.024 N IGF1R n/a
6 TRCN0000121192 CCTGAATCTGTGCAAACAGTA pLKO.1 3607 3UTR 100% 4.950 3.960 N IGF1R n/a
7 TRCN0000121298 CGACAGACACTCAGGACACAA pLKO.1 3444 CDS 100% 4.950 3.960 N IGF1R n/a
8 TRCN0000121301 CGGCAACCTGAGTTACTACAT pLKO.1 1407 CDS 100% 4.950 3.960 N IGF1R n/a
9 TRCN0000195002 CAACACTTAATAGCAACAGAG pLKO.1 3852 3UTR 100% 4.050 3.240 N IGF1R n/a
10 TRCN0000018331 CATGTACTGCATCCCTTGTGA pLKO.1 447 CDS 100% 3.000 2.400 N IGF1R n/a
11 TRCN0000121134 CAAGGGCAATTTGCTCATTAA pLKO.1 561 CDS 100% 13.200 9.240 N IGF1R n/a
12 TRCN0000199517 GCGCATGTGCTGGCAGTATAA pLKO.1 3228 CDS 100% 13.200 9.240 N IGF1R n/a
13 TRCN0000000423 GCTACCTTTACCGGCACAATT pLKO.1 1457 CDS 100% 13.200 9.240 N IGF1R n/a
14 TRCN0000121195 GTCTTCCATAGAAAGAGAAAT pLKO.1 2362 CDS 100% 13.200 9.240 N IGF1R n/a
15 TRCN0000000426 CCAAGCCTGAGCAAGATGATT pLKO.1 2812 CDS 100% 5.625 3.938 N IGF1R n/a
16 TRCN0000121132 CCTTTATCTTTCACCTTTCTA pLKO.1 4096 3UTR 100% 5.625 3.938 N IGF1R n/a
17 TRCN0000121135 GAGACAGAGTACCCTTTCTTT pLKO.1 1807 CDS 100% 5.625 3.938 N IGF1R n/a
18 TRCN0000121136 CCAAGGATGCACCATCTTCAA pLKO.1 543 CDS 100% 4.950 3.465 N IGF1R n/a
19 TRCN0000039675 GCCGAAGATTTCACAGTCAAA pLKO.1 2923 CDS 100% 4.950 3.465 N IGF1R n/a
20 TRCN0000039676 CCAAATTATGTGTTTCCGAAA pLKO.1 848 CDS 100% 4.050 2.835 N IGF1R n/a
21 TRCN0000000422 CCTTAACTGACATGGGCCTTT pLKO.1 3817 3UTR 100% 4.050 2.835 N IGF1R n/a
22 TRCN0000121299 CTTCTACTACAGCGAGGAGAA pLKO.1 3327 CDS 100% 4.050 2.835 N IGF1R n/a
23 TRCN0000000425 CCTTGGACGTTCTTTCAGCAT pLKO.1 1334 CDS 100% 2.640 1.848 N IGF1R n/a
24 TRCN0000010361 AAACACATTTGGGATGTTCCT pLKO.1 3756 3UTR 100% 0.000 0.000 N IGF1R n/a
25 TRCN0000039673 AACACATTTGGGATGTTCCTC pLKO.1 3757 3UTR 100% 0.000 0.000 N IGF1R n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011521516.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14671 pDONR223 0% 77.8% 77.8% None 0_1ins909 n/a
2 ccsbBroad304_14671 pLX_304 0% 77.8% 77.8% V5 0_1ins909 n/a
3 TRCN0000473406 AAAATCAGCCCATACACAGGGTTC pLX_317 10.2% 77.8% 77.8% V5 0_1ins909;3075C>T n/a
4 TRCN0000489071 TCACACATTATGCGGGCCCCCCCC pLX_317 7% 77.8% 77.8% V5 (not translated due to prior stop codon) 0_1ins909 n/a
5 TRCN0000489368 CCGATGCCTGTCGCCGTCTGGTGA pLX_317 8.2% 77.8% 77.7% V5 0_1ins909;3192_3193insG n/a
6 TRCN0000489182 GGGCACACGGTCTCTGTGCCATAC pLX_317 33.6% 35.9% .4% V5 (not translated due to prior stop codon) 1_2044del n/a
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