Construct: ORF TRCN0000473412
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018926.2_s317c1
- Derived from:
- ccsbBroadEn_15028
- DNA Barcode:
- CTACGTAAAGGCGACCATTCGTTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKD2 (25865)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473412
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25865 | PRKD2 | protein kinase D2 | NM_001079880.1 | 99.6% | 29.6% | (many diffs) |
| 2 | human | 25865 | PRKD2 | protein kinase D2 | NM_001079881.1 | 99.6% | 29.6% | (many diffs) |
| 3 | human | 25865 | PRKD2 | protein kinase D2 | NM_016457.4 | 99.6% | 29.6% | (many diffs) |
| 4 | human | 25865 | PRKD2 | protein kinase D2 | NM_001079882.1 | 81.7% | 12.4% | (many diffs) |
| 5 | human | 25865 | PRKD2 | protein kinase D2 | XM_005258716.2 | 81.7% | 12.4% | (many diffs) |
| 6 | mouse | 101540 | Prkd2 | protein kinase D2 | NM_001252458.1 | 87.8% | 28.6% | (many diffs) |
| 7 | mouse | 101540 | Prkd2 | protein kinase D2 | NM_178900.4 | 87.8% | 28.6% | (many diffs) |
| 8 | mouse | 101540 | Prkd2 | protein kinase D2 | XR_391036.1 | 60.2% | (many diffs) | |
| 9 | mouse | 101540 | Prkd2 | protein kinase D2 | XM_006539444.1 | 55.2% | 11.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1209
- ORF length:
- 1140
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccaccgcc ccctcttatc ccgccgggct ccctggctct cccgggccgg 121 ggtctcctcc gccccccggc ggcctagagc tgcagtcgcc gccaccgcta ctgccccaga 181 tcccggcccc gggttccggg gtctcctttc acatccagat cgggctgacc cgcgagttcg 241 tgctgttgcc cgccgcctcc gagctggctc atgtgaagca gctggcctgt tccatcgtgg 301 accagaagtt ccctgagtgt ggcttctacg gcctttacga caagatcctg cttttcaaac 361 atgaccccac gtcggccaac ctcctgcagc tggtgcgctc gtccggagac atccaggagg 421 gcgacctggt ggaggtggtg ctgtcggcct cggccacctt cgaggacttc cagatccgcc 481 cgcacgccct cacggtgcac tcctatcggg cgcctgcctt ctgtgatcac tgcggggaga 541 tgctcttcgg cctagtgcgc cagggcctca agtgcgatgg ctgcgggctg aactaccaca 601 agcgctgtgc cttcagcatc cccaacaact gtagtggggc ccgcaaacgg cgcctgtcat 661 ccacgtctct ggccagtggc cactcggtgc gcctcggcac ctccgagtcc ctgccctgca 721 cggctgaaga gctgagccgt agcaccaccg aactcctgcc tcgccgtccc ccgtcatcct 781 cttcctcctc ttctgcctca tcgtatacgg gccgccccat tgagctggac agatgctgct 841 ccccaaggtc aaggtgccgc acaccttcct catccacagc tatacacggc ccaccgtttg 901 ccaggcttgc aagaaactcc tcaagggcct cttccggcag ggcctgcaat gcaaagactg 961 caagtttaac tgtcacaaac gctgcgccac ccgcgtccct aatgactgcc tgggggaggc 1021 ccttatcaat ggagatgtgc cgatggagga ggccaccgat ttcagcgagg ctgacaagag 1081 cgccctcatg gatgagtcag aggactccgg tgtcatccct ggctcccact cagagaatgc 1141 gctccacgcc agtgaggagg aggaaggcga gggaggcaag gcccagagct ccctggggta 1201 catcccccta atgagggtgg tgcaatcggt gcgacacacg acgcggaaat ccagcaccac 1261 gctgcgggag ggttgggtgg ttcattacag caacaaggac acgctgagaa agcggcacta 1321 ttggcgcctg gactgcaagt gtatcacgct cttccagaac aacacgacca acagatacta 1381 taaggaaatt ccgctgtcag aaatcctcac ggtggagtcc gcccagaact tcagccttgt 1441 gccgccgggc accaacccac actgctttga gatcgtcact gccaatgcca cctacttcgt 1501 gggcgagatg cctggcggga ctccgggtgg gccaagtggg cagggggctg aggccgcccg 1561 gggctgggag acagccatcc gccaggccct gatgcccgtc atccttcagg acgcacccag 1621 cgccccaggc cacgcgcccc acagacaagc ttctctgagc atctctgtgt ccaacagtca 1681 gatccaagag aatgtggaca ttgccactgt ctaccagatc ttccctgacg aagtgctggg 1741 ctcagggcag tttggagtgg tctatggagg aaaacaccgg aagacaggcc gggacgtggc 1801 agttaaggtc attgacaaac tgcgcttccc taccaagcag gagagccagc tccggaatga 1861 agtggccatt ctgcagagcc tgcggcatcc cgggatcgtg aacctggagt gcatgttcga 1921 gacgcctgag aaagtgtttg tgtggatgag aaagctgcat gggggacagg tgggagatga 1981 tcctgtccag tgagaagggc cggctgcctg agcgcctcac caagttcctc atcacccaga 2041 tcctggtggc ttngagacac cttcacttca agaacattgt ccactgtgac ttgaaaccag 2101 aaaacgtgtt gctggcatca gcagacccat ttcctcaggt gaagctgtgt gactttggct 2161 ttgctcgcat catcggcgag aagtcgttcc gccgctcagt ggtgggcacg ccggcctacc 2221 tggcacccga ggtgctgctc aaccagggct acaaccgctc gctggacatg tggtcagtgg 2281 gcgtgatcat gtacgtcagc ctcagcggca ccTTCCCTTT CAACGAGGAT GAGGACATCA 2341 ATGACCAGAT CCAGAACGCC GCCTTCATGT ACCCGGCCAG CCCCTGGAGC CACATCTCAG 2401 CTGGAGCCAT TGACCTCATC AACAACCTGC TGCAGGTGAA GATGCGCAAA CGCTACAGCG 2461 TGGACAAATC TCTCAGCCAC CCCTGGTTAC AGGAGTACCA GACGTGGCTG GACCTCCGAG 2521 AGCTGGAGGG GAAGATGGGA GAGCGATACA TCACGCATGA GAGTGACGAC GCGCGCTGGG 2581 AGCAGTTTGC AGCAGAGCAT CCGCTGCCTG GGTCTGGGCT GCCCACGGAC AGGGATCTCG 2641 GTGGGGCCTG TCCACCACAG GACCACGACA TGCAGGGGCT GGCGGAGCGC ATCAGTGTTC 2701 TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2761 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2821 GGCTTTATAT ATCTTGTGGA AAGGACGACT ACGTAAAGGC GACCATTCGT TGACGCGTTA 2881 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt