Construct: ORF TRCN0000473426
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015984.1_s317c1
- Derived from:
- ccsbBroadEn_11391
- DNA Barcode:
- GTTAGAAGAAACTTATTTCCATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APOBEC3B (9582)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473426
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XM_017028642.2 | 77.9% | 64.7% | (many diffs) |
| 2 | human | 9582 | APOBEC3B | apolipoprotein B mRNA editi... | NM_001270411.2 | 68.2% | 54.5% | (many diffs) |
| 3 | human | 9582 | APOBEC3B | apolipoprotein B mRNA editi... | NM_004900.5 | 65.4% | 51.1% | (many diffs) |
| 4 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | NM_145298.6 | 57.6% | 42.8% | (many diffs) |
| 5 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XM_024452179.1 | 57.6% | 42.8% | (many diffs) |
| 6 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | NM_152426.4 | 55.9% | 42.2% | (many diffs) |
| 7 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XM_024452178.1 | 48.7% | 36.5% | (many diffs) |
| 8 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | XM_017028596.2 | 47.5% | 36.1% | (many diffs) |
| 9 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | XR_001755170.2 | 27.1% | (many diffs) | |
| 10 | human | 200315 | APOBEC3A | apolipoprotein B mRNA editi... | NM_001270406.2 | 26.5% | 25.6% | (many diffs) |
| 11 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | XR_001755169.2 | 24.9% | (many diffs) | |
| 12 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XR_002958675.1 | 14.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 819
- ORF length:
- 753
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tccacagatc agaaatccga tggagcggat gtatcgagac acattctacg 121 acaactttga aaacgaaccc atcctctatg gtcggagcta cacttggctg tgctatgaag 181 tgaaaataaa gaggggccgc tcaaatctcc tttgggacac aggggtcttt cgaggccagg 241 tgtatttcga gcctcagtac cacgcagaaa tgtgcttcct ctcttggttc tgtggcaacc 301 agctgcctgc ttacaagtgt ttccagatca cctggtttgt atcctggacc ccctgcccgg 361 actgtgtggc gaagctggcc gaattcctgt ctgagcaccc caatgtcacc ctgaccatct 421 ccgccgcccg cctctactac tactgggaaa gagattaccg aagggcgctc tgcaggctga 481 gtcaggcagg agcccgcgtg aagatcatgg actatgaaga atttgcatac tgctgggaaa 541 actttgtgta caatgaaggt cagcaattca tgccttggta caaattcgat gaaaattatg 601 cattccTGCA CCGCACGCTA AAGGAGATTC TCAGGCTAAG AATCTTCTCT GTGGCTTTTA 661 CGGCCGCCAT GCGGAGCTGC GCTTCTTGGA CCTGGTTCCT TCTTTGCAGT TGGACCCGGC 721 CCAGATCTAC AGGGTCACTT GGTTCATCTC CTGGAGCCCC TGCTTCTCCT GGGGCTGTGC 781 CGGGGAAGTG CGTGCGTTCC TTCAGGAGAA CACACACGTG CCCAACTTTC TTGTACAAAG 841 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 901 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 961 ACGAGTTAGA AGAAACTTAT TTCCATTAAC GCGTTAAGTC gacaatcaac ctctggatta 1021 caaaatttgt gaaagatt