Construct: ORF TRCN0000473451
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001176.1_s317c1
- Derived from:
- ccsbBroadEn_06685
- DNA Barcode:
- TCCATCCACTCCCCCTTCTAACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAH (5053)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473451
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5053 | PAH | phenylalanine hydroxylase | NM_000277.3 | 99.8% | 99.7% | 108A>G;548A>G |
| 2 | human | 5053 | PAH | phenylalanine hydroxylase | NM_001354304.2 | 99.8% | 99.7% | 108A>G;548A>G |
| 3 | human | 5053 | PAH | phenylalanine hydroxylase | XM_017019370.2 | 52.4% | 51.7% | (many diffs) |
| 4 | mouse | 18478 | Pah | phenylalanine hydroxylase | NM_008777.3 | 87% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1422
- ORF length:
- 1356
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cactgcggtc ctggaaaacc caggcttggg caggaaactc tctgactttg 121 gacaggaaac aagctatatt gaagacaact gcaatcaaaa tggtgccata tcgctgatct 181 tctcactcaa agaagaagtt ggtgcattgg ccaaagtatt gcgcttattt gaggagaatg 241 atgtaaacct gacccacatt gaatctagac cttctcgttt aaagaaagat gagtatgaat 301 ttttcaccca tttggataaa cgtagcctgc ctgctctgac aaacatcatc aagatcttga 361 ggcatgacat tggtgccact gtccatgagc tttcacgaga taagaagaaa gacacagtgc 421 cctggttccc aagaaccatt caagagctgg acagatttgc caatcagatt ctcagctatg 481 gagcggaact ggatgctgac caccctggtt ttaaagatcc tgtgtaccgt gcaagacgga 541 agcagtttgc tgacattgcc tacaactacc gccatgggca gcccatccct cgagtggaat 601 acatggagga aggaaagaaa acatggggca cagtgttcaa gactctgaag tccttgtata 661 aaacccatgc ttgctatgag tacaatcaca tttttccact tcttgaaaag tactgtggct 721 tccatgaaga taacattccc cagctggaag acgtttctca gttcctgcag acttgcactg 781 gtttccgcct ccgacctgtg gctggcctgc tttcctctcg ggatttcttg ggtggcctgg 841 ccttccgagt cttccactgc acacagtaca tcagacatgg atccaagccc atgtataccc 901 ccgaacctga catctgccat gagctgttgg gacatgtgcc cttgttttca gatcgcagct 961 ttgccCAGTT TTCCCAGGAA ATTGGCCTTG CCTCTCTGGG TGCACCTGAT GAATACATTG 1021 AAAAGCTCGC CACAATTTAC TGGTTTACTG TGGAGTTTGG GCTCTGCAAA CAAGGAGACT 1081 CCATAAAGGC ATATGGTGCT GGGCTCCTGT CATCCTTTGG TGAATTACAG TACTGCTTAT 1141 CAGAGAAGCC AAAGCTTCTC CCCCTGGAGC TGGAGAAGAC AGCCATCCAA AATTACACTG 1201 TCACGGAGTT CCAGCCCCTC TATTACGTGG CAGAGAGTTT TAATGATGCC AAGGAGAAAG 1261 TAAGGAACTT TGCTGCCACA ATACCTCGGC CCTTCTCAGT TCGCTACGAC CCATACACCC 1321 AAAGGATTGA GGTCTTGGAC AATACCCAGC AGCTTAAGAT TTTGGCTGAT TCCATTAACA 1381 GTGAAATTGG AATCCTTTGC AGTGCCCTCC AGAAAATAAA GTACCCAACT TTCTTGTACA 1441 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1501 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1561 AGGACGATCC ATCCACTCCC CCTTCTAACA AACGCGTTAA GTCgacaatc aacctctgga 1621 ttacaaaatt tgtgaaagat t