Construct: ORF TRCN0000473475
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011508.1_s317c1
- Derived from:
- ccsbBroadEn_00719
- DNA Barcode:
- ACAACCTAGAGAAGGGCCGCGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H3-3B (3021)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473475
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3021 | H3-3B | H3.3 histone B | NM_005324.5 | 100% | 100% | |
| 2 | human | 440093 | H3-5 | H3.5 histone | NM_001013699.3 | 96.5% | 95.5% | (many diffs) |
| 3 | human | 8355 | H3C8 | H3 clustered histone 8 | NM_003534.2 | 80.8% | 96.3% | (many diffs) |
| 4 | human | 333932 | H3C15 | H3 clustered histone 15 | NM_001005464.2 | 80.5% | 97% | (many diffs) |
| 5 | human | 126961 | H3C14 | H3 clustered histone 14 | NM_021059.2 | 80.5% | 97% | (many diffs) |
| 6 | human | 653604 | H3C13 | H3 clustered histone 13 | NM_001123375.2 | 80.3% | 97% | (many diffs) |
| 7 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001355409.3 | 80.1% | 94.1% | (many diffs) |
| 8 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001372105.1 | 80.1% | 94.1% | (many diffs) |
| 9 | human | 8351 | H3C4 | H3 clustered histone 4 | NM_003530.4 | 79.8% | 96.3% | (many diffs) |
| 10 | human | 8352 | H3C3 | H3 clustered histone 3 | NM_003531.2 | 79.7% | 96.3% | (many diffs) |
| 11 | human | 8356 | H3C12 | H3 clustered histone 12 | NM_003535.2 | 79.1% | 96.3% | (many diffs) |
| 12 | human | 8354 | H3C11 | H3 clustered histone 11 | NM_003533.2 | 79.1% | 96.3% | (many diffs) |
| 13 | human | 8357 | H3C10 | H3 clustered histone 10 | NM_003536.2 | 79.1% | 96.3% | (many diffs) |
| 14 | human | 8290 | H3-4 | H3.4 histone | NM_003493.2 | 79.1% | 93.3% | (many diffs) |
| 15 | human | 3020 | H3-3A | H3.3 histone A | NM_002107.6 | 78.4% | 100% | (many diffs) |
| 16 | human | 8968 | H3C7 | H3 clustered histone 7 | NM_021018.2 | 78% | 96.3% | (many diffs) |
| 17 | mouse | 15081 | H3f3b | H3 histone, family 3B | NM_008211.3 | 91.9% | 100% | (many diffs) |
| 18 | mouse | 15081 | H3f3b | H3 histone, family 3B | XM_006532248.2 | 91.9% | 100% | (many diffs) |
| 19 | mouse | 97114 | Hist2h3c2 | histone cluster 2, H3c2 | NM_054045.4 | 83% | 97% | (many diffs) |
| 20 | mouse | 15077 | Hist2h3c1 | histone cluster 2, H3c1 | NM_178216.3 | 83% | 97% | (many diffs) |
| 21 | mouse | 319148 | Hist1h3c | histone cluster 1, H3c | NM_175653.2 | 82.8% | 97% | (many diffs) |
| 22 | mouse | 319150 | Hist1h3b | histone cluster 1, H3b | NM_178203.2 | 82.8% | 97% | (many diffs) |
| 23 | mouse | 319149 | Hist1h3d | histone cluster 1, H3d | NM_178204.2 | 82.8% | 97% | (many diffs) |
| 24 | mouse | 319154 | Hist2h3b | histone cluster 2, H3b | NM_178215.2 | 82.8% | 97% | (many diffs) |
| 25 | mouse | 319152 | Hist1h3h | histone cluster 1, H3h | NM_178206.2 | 82.5% | 96.3% | (many diffs) |
| 26 | mouse | 260423 | Hist1h3f | histone cluster 1, H3f | NM_013548.4 | 82.3% | 97% | (many diffs) |
| 27 | mouse | 319151 | Hist1h3e | histone cluster 1, H3e | NM_178205.2 | 82.3% | 97% | (many diffs) |
| 28 | mouse | 360198 | Hist1h3a | histone cluster 1, H3a | NM_013550.5 | 82.1% | 96.3% | (many diffs) |
| 29 | mouse | 97908 | Hist1h3g | histone cluster 1, H3g | NM_145073.2 | 81.8% | 96.3% | (many diffs) |
| 30 | mouse | 319153 | Hist1h3i | histone cluster 1, H3i | NM_178207.2 | 81.8% | 96.3% | (many diffs) |
| 31 | mouse | 382523 | Gm12260 | predicted gene 12260 | NM_001318003.2 | 78.8% | 94.1% | (many diffs) |
| 32 | mouse | 624251 | Gm6485 | predicted gene 6485 | XR_375938.3 | 39.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 474
- ORF length:
- 408
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccgaaccaag cagactgctc GTAAGTCCAC CGGTGGGAAA GCCCCCCGCA 121 AACAGCTGGC CACGAAAGCC GCCAGGAAAA GCGCTCCCTC TACCGGCGGG GTGAAGAAGC 181 CTCATCGCTA CAGGCCCGGG ACCGTGGCGC TTCGAGAGAT TCGTCGTTAT CAGAAGTCGA 241 CCGAGCTGCT CATCCGGAAG CTGCCCTTCC AGAGGTTGGT GAGGGAGATC GCGCAGGATT 301 TCAAAACCGA CCTGAGGTTT CAGAGCGCAG CCATCGGTGC GCTGCAGGAG GCTAGCGAAG 361 CGTACCTGGT GGGTCTGTTC GAAGATACCA ACCTGTGTGC CATCCACGCT AAGAGAGTCA 421 CCATCATGCC CAAAGACATC CAGTTGGCTC GCCGGATACG GGGAGAGAGA GCTTACCCAA 481 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 541 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 601 TATCTTGTGG AAAGGACGAA CAACCTAGAG AAGGGCCGCG TGAACGCGTT AAGTCgacaa 661 tcaacctctg gattacaaaa tttgtgaaag att