Construct: ORF TRCN0000473537
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018331.1_s317c1
- Derived from:
- ccsbBroadEn_02633
- DNA Barcode:
- AGCGTGGCTAAGTCTAATTGTTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LSM6 (11157)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473537
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11157 | LSM6 | LSM6 homolog, U6 small nucl... | NM_007080.3 | 100% | 100% | |
| 2 | human | 11157 | LSM6 | LSM6 homolog, U6 small nucl... | XM_017007672.1 | 100% | 100% | |
| 3 | human | 11157 | LSM6 | LSM6 homolog, U6 small nucl... | XM_017007673.1 | 100% | 100% | |
| 4 | human | 11157 | LSM6 | LSM6 homolog, U6 small nucl... | XR_001741098.1 | 27.4% | 1_119del;360_874del | |
| 5 | human | 11157 | LSM6 | LSM6 homolog, U6 small nucl... | XR_001741100.1 | 8.6% | 1_119del;360_2771del | |
| 6 | human | 11157 | LSM6 | LSM6 homolog, U6 small nucl... | XR_001741099.1 | 8.4% | 1_119del;360_2830del | |
| 7 | mouse | 78651 | Lsm6 | LSM6 homolog, U6 small nucl... | NM_001191004.1 | 92% | 100% | (many diffs) |
| 8 | mouse | 78651 | Lsm6 | LSM6 homolog, U6 small nucl... | NM_030145.3 | 92% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 216
- ORF end:
- 456
- ORF length:
- 240
- Sequence:
-
1 GTTTTATGCG ATGGAGTTTC CCCACACTGA GTGGGTGGAG ACTGAAGTTA GGCCAGCTTG 61 GCACTTGATG TAATTCTCCT TGGAATTTGC CCTTTTTGAG TTTGGATCTT GGTTCATTCT 121 CAAGCCTCAG ACAGTGGTTC AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGCTAGCA 181 TCGATTGATC AACAAGTTTG TACAAAAAAG TTGGCATGAG TCTTCGGAAG CAAACCCCTA 241 GTGACTTCTT AAAGCAAATC ATCGGACGAC CAGTTGTGGT AAAATTAAAT TCTGGAGTGG 301 ATTATCGAGG GGTCCTGGCT TGCCTGGATG GCTACATGAA TATAGCCCTG GAGCAGACAG 361 AAGAATATGT AAATGGACAA CTGAAGAATA AGTATGGGGA TGCATTTATC CGAGGAAACA 421 ATGTGTTGTA CATCAGTACA CAGAAGAGAC GGATGTGCCC AACTTTCTTG TACAAAGTGG 481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 601 AAGCGTGGCT AAGTCTAATT GTTAAACGCG TTAAGTCgac aatcaacctc tggattacaa 661 aatttgtgaa agatt