Construct: ORF TRCN0000473547
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018791.2_s317c1
- Derived from:
- ccsbBroadEn_14599
- DNA Barcode:
- CTCATCCGGTGCGATGATGCCAAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CSNK1A1 (1452)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473547
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1452 | CSNK1A1 | casein kinase 1 alpha 1 | NM_001892.6 | 99.4% | 22.5% | 226_226delAinsCAN;229_230delCGinsNN;414_415insC |
| 2 | human | 1452 | CSNK1A1 | casein kinase 1 alpha 1 | NM_001271741.2 | 95.2% | 23.3% | (many diffs) |
| 3 | human | 1452 | CSNK1A1 | casein kinase 1 alpha 1 | NM_001025105.3 | 91.8% | 20.8% | (many diffs) |
| 4 | human | 122011 | CSNK1A1L | casein kinase 1 alpha 1 like | NM_145203.6 | 90.8% | 18.6% | (many diffs) |
| 5 | human | 1452 | CSNK1A1 | casein kinase 1 alpha 1 | NM_001271742.2 | 67.7% | 6% | 0_1ins269;147_148insC;188_271del |
| 6 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526388.2 | 94.3% | 22.5% | (many diffs) |
| 7 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | NM_146087.2 | 90.2% | 23.3% | (many diffs) |
| 8 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526384.2 | 87.1% | 20.8% | (many diffs) |
| 9 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526385.2 | 86.8% | 20.8% | (many diffs) |
| 10 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_011247048.2 | 84.9% | 20.3% | (many diffs) |
| 11 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526383.3 | 84.8% | 20.3% | (many diffs) |
| 12 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526386.3 | 84.5% | 20.9% | (many diffs) |
| 13 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XM_006526387.2 | 83.3% | 21.5% | (many diffs) |
| 14 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XR_386023.3 | 13.7% | (many diffs) | |
| 15 | mouse | 93687 | Csnk1a1 | casein kinase 1, alpha 1 | XR_386022.3 | 13.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 333
- ORF length:
- 264
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgagtagc agcggctcca aggctgaatt cattgtcgga gggaaatata 121 aactggtacg gaagatcggg tctggctcct tcggggacat ctatttggcg atcaacatca 181 ccaacggcga ggaagtggca gtgaagctag aatctcagaa ggccaggcat ccccagttgc 241 tgtacgagag caagctctat aagattcttc aaggtggggt tggcatcccc caccantann 301 gtggtatggt caggaaaaag actacaatgt actagtcatg gatcttctgg gacctagcct 361 cgaagacctc ttcaatttct gttcaagaag gttcacaatg aaaactgtac ttatgttagc 421 tgaccagatg atcagtagaa ttgaatatgt gcatacaaag aattttatac acagagacat 481 taaacccaga taacttccta atgggtattg ggcgtcactg taataagtta ttccttattg 541 attttggttt ggccaaaaag tacagagaca acaggacaag gcaacacata ccatacagag 601 aagataaaaa cctcactggc actgcccgat atgctagcat caatgcacat cttGGTATTG 661 AGCAGAGTCG CCGAGATGAC ATGGAATCAT TAGGATATGT TTTGATGTAT TTTAATAGAA 721 CCAGCCTGCC ATGGCAAGGG CTAAAGGCTG CAACAAAGAA ACAAAAATAT GAAAAGATTA 781 GTGAAAAGAA GATGTCCACG CCTGTTGAAG TTTTATGTAA GGGGTTTCCT GCAGAATTTG 841 CGATGTACTT AAACTATTGT CGTGGGCTAC GCTTTGAGGA AGCCCCAGAT TACATGTATC 901 TGAGGCAGCT ATTCCGCATT CTTTTCAGGA CCCTGAACCA TCAATATGAC TACACATTTG 961 ATTGGACAAT GTTAAAGCAG AAAGCAGCAC AGCAGGCAGC CTCTTCCAGT GGGCAGGGTC 1021 AGCAGGCCCA AACCCCCACA GGCAAGCAAA CTGACAAAAC CAAGAGTAAC ATGAAAGGTT 1081 TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1141 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1201 GGCTTTATAT ATCTTGTGGA AAGGACGACT CATCCGGTGC GATGATGCCA AAACGCGTTA 1261 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt