Construct: ORF TRCN0000473566
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014978.1_s317c1
- Derived from:
- ccsbBroadEn_12272
- DNA Barcode:
- CCTTGGAACCGATTTTTTGTAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MBD5 (55777)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473566
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55777 | MBD5 | methyl-CpG binding domain p... | NM_018328.4 | 14.9% | 14.7% | (many diffs) |
| 2 | human | 55777 | MBD5 | methyl-CpG binding domain p... | XM_024452990.1 | 14.8% | 14.5% | (many diffs) |
| 3 | human | 55777 | MBD5 | methyl-CpG binding domain p... | XR_002959319.1 | 13.7% | (many diffs) | |
| 4 | human | 55777 | MBD5 | methyl-CpG binding domain p... | XM_024452989.1 | 13.1% | 12.9% | (many diffs) |
| 5 | human | 55777 | MBD5 | methyl-CpG binding domain p... | XM_024452988.1 | 13% | 12.8% | (many diffs) |
| 6 | human | 55777 | MBD5 | methyl-CpG binding domain p... | XM_024452987.1 | 12.9% | 12.7% | (many diffs) |
| 7 | human | 55777 | MBD5 | methyl-CpG binding domain p... | XM_011511470.2 | 12.8% | 12.6% | (many diffs) |
| 8 | human | 55777 | MBD5 | methyl-CpG binding domain p... | XM_011511472.2 | 12.8% | 12.6% | (many diffs) |
| 9 | human | 55777 | MBD5 | methyl-CpG binding domain p... | XR_002959318.1 | 12.2% | (many diffs) | |
| 10 | mouse | 109241 | Mbd5 | methyl-CpG binding domain p... | XM_017314916.1 | 16.3% | 16% | (many diffs) |
| 11 | mouse | 109241 | Mbd5 | methyl-CpG binding domain p... | NM_001290656.1 | 14.4% | 14.2% | (many diffs) |
| 12 | mouse | 109241 | Mbd5 | methyl-CpG binding domain p... | XM_017314915.1 | 14.4% | 14.2% | (many diffs) |
| 13 | mouse | 109241 | Mbd5 | methyl-CpG binding domain p... | XM_006497604.2 | 13.4% | 13.2% | (many diffs) |
| 14 | mouse | 109241 | Mbd5 | methyl-CpG binding domain p... | XM_017314914.1 | 12.9% | 12.7% | (many diffs) |
| 15 | mouse | 109241 | Mbd5 | methyl-CpG binding domain p... | NM_029924.2 | 12.5% | 12.3% | (many diffs) |
| 16 | mouse | 109241 | Mbd5 | methyl-CpG binding domain p... | XM_017314909.1 | 12.5% | 12.3% | (many diffs) |
| 17 | mouse | 109241 | Mbd5 | methyl-CpG binding domain p... | XM_017314910.1 | 12.5% | 12.3% | (many diffs) |
| 18 | mouse | 109241 | Mbd5 | methyl-CpG binding domain p... | XM_017314911.1 | 12.5% | 12.3% | (many diffs) |
| 19 | mouse | 109241 | Mbd5 | methyl-CpG binding domain p... | XM_017314912.1 | 12.5% | 12.3% | (many diffs) |
| 20 | mouse | 109241 | Mbd5 | methyl-CpG binding domain p... | XR_001780404.1 | 6.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 753
- ORF length:
- 687
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ccctcctact gccaacatgc ttctcccaac aggtgaaggg caaagtggtc 121 gagcagcact aagagataag ctgatgtctc agcaaaaaga cgcattgcgg aaaagaaaac 181 aaccacctac gacagtgttg agtttgctca gacagtctca aatggatagt tctgcagttc 241 ctaaacctgg acctgacttg ctaaggaagc agggtcaggg ttcatttccC ATCAGTTCAA 301 TGTCTCAGTT ACTACAGTCT ATGAGTTGTC AAAGCTCTCA CTTGAGTAGC AATAGTACCC 361 CGGGTTGTGG GGCCTCAAAT ACTGCTTTGC CTTGCTCTGC TAACCAGCTG CATTTTACAG 421 ATCCCAGTAT GAACTCTAGT GTTCTTCAGA ACATACCTTT AAGAGGGGAA GCCGTGCACT 481 GCCACAATGC AAACACTAAC TTTGTTCACA GTAACAGTCC AGTCCCCAAC CACCATCTTG 541 CAGGTTTAAT AAATCAGATT CAGGCTAGCG GGAACTGTGG GATGCTCAGT CAGTCGGGCA 601 TGGCTTTAGG AAATTCCTTA CATCCCAATC CACCTCAGTC AAGAATTTCA ACGTCCTCCA 661 CTCCAGTGAT ACCAAACAGC ATTGTTAGCA GCTATAATCA AACAAGTTCT GAAGCAGGTA 721 TGGTTTTATT AGAAAAAAGT ACCCAAAGGT ACTACCCAAC TTTCTTGTAC AAAGTGGTTG 781 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 841 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 901 TTGGAACCGA TTTTTTGTAA AGACGCGTTA AGTCgacaat caacctctgg attacaaaat 961 ttgtgaaaga tt