Construct: ORF TRCN0000473601
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017408.1_s317c1
- Derived from:
- ccsbBroadEn_11397
- DNA Barcode:
- TCACCCAGAGTGGGACCCCTTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDE4DIP (9659)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473601
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001195261.1 | 98.8% | 97.7% | (many diffs) |
| 2 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001002810.3 | 95.1% | 92% | (many diffs) |
| 3 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001195260.1 | 79% | 77.9% | (many diffs) |
| 4 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_022359.6 | 54.8% | 51.9% | (many diffs) |
| 5 | human | 653513 | LOC653513 | phosphodiesterase 4D intera... | NR_037182.1 | 41.8% | (many diffs) | |
| 6 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001002812.2 | 17.4% | 16.7% | (many diffs) |
| 7 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001198832.2 | 7.6% | 7.2% | (many diffs) |
| 8 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002903.2 | 7.6% | 7.2% | (many diffs) |
| 9 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001350523.1 | 7.6% | 7.1% | (many diffs) |
| 10 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002899.2 | 7.4% | 7% | (many diffs) |
| 11 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510176.2 | 7.4% | 7% | (many diffs) |
| 12 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_024451068.1 | 7.3% | 6.9% | (many diffs) |
| 13 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001350522.1 | 7.3% | 6.9% | (many diffs) |
| 14 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510179.1 | 7.2% | 6.9% | (many diffs) |
| 15 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_006711650.4 | 7.2% | 7.1% | (many diffs) |
| 16 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_014644.5 | 7.2% | 6.9% | (many diffs) |
| 17 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001198834.3 | 7.1% | 6.8% | (many diffs) |
| 18 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_024451067.1 | 7.1% | 6.7% | (many diffs) |
| 19 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_006711652.1 | 6.9% | 6.7% | (many diffs) |
| 20 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001350521.2 | 6.9% | 6.5% | (many diffs) |
| 21 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_006711651.1 | 6.9% | 6.7% | (many diffs) |
| 22 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510175.2 | 6.9% | 6.5% | (many diffs) |
| 23 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002878.2 | 6.9% | 6.5% | (many diffs) |
| 24 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_006711646.2 | 6.8% | 6.4% | (many diffs) |
| 25 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510173.2 | 6.7% | 6.3% | (many diffs) |
| 26 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510172.2 | 6.7% | 6.3% | (many diffs) |
| 27 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002887.1 | 5.7% | 5.6% | (many diffs) |
| 28 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002888.1 | 5.7% | 5.6% | (many diffs) |
| 29 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002889.2 | 5.7% | 5.6% | (many diffs) |
| 30 | human | 100996724 | LOC100996724 | phosphodiesterase 4D intera... | NR_144517.1 | 4.8% | (many diffs) | |
| 31 | human | 100996724 | LOC100996724 | phosphodiesterase 4D intera... | NR_144516.1 | 4.7% | (many diffs) | |
| 32 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002891.2 | 4.5% | 4% | (many diffs) |
| 33 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002892.2 | 4.5% | 4% | (many diffs) |
| 34 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002893.1 | 4.5% | 4% | (many diffs) |
| 35 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002894.2 | 4.5% | 4% | (many diffs) |
| 36 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002895.2 | 4.5% | 4% | (many diffs) |
| 37 | mouse | 83679 | Pde4dip | phosphodiesterase 4D intera... | NM_001289702.1 | 6.3% | 5.6% | (many diffs) |
| 38 | mouse | 83679 | Pde4dip | phosphodiesterase 4D intera... | NM_001039376.2 | 6.2% | 5.5% | (many diffs) |
| 39 | mouse | 83679 | Pde4dip | phosphodiesterase 4D intera... | NM_001289701.1 | 6.1% | 5.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 597
- ORF length:
- 531
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cctctgtcag ggattccgca aatatctccc tgagcacctc aatgacctga 121 agaaggagaa cttcagcctc aagctgcgca tctacttcct GGAGGAGCGC ATGCAACAGA 181 AGTATGAGGC CAGCCGGGAG GACATCTACA GGCGGAACAC TGAGCTGAAG GTTGAAGTGG 241 AGAGCTTGAA ACGAGAACTC CAGGACAAGA AACAGCATCT GGATAAAACA TGGGCTGATG 301 TGGAGAATCT CAACAGTCAG AATGAAGCTG AGCTCCGACG CCAGTTTGAG GAGCGACAGC 361 AGGAGACGGA GCATGTTTAT GAGCTCTTGG AGAATAAGAT GCAGCTTCTG CAGGAGGAAT 421 CCAGGCTAGC AAAGAATGAA GCTGCGCGGA TGGCAGCTCT GGTGGAAGCA GAGAAGGAGT 481 GTAACCTGGA GCTCTCAGAG AAACTGAAGG GAGTCACCAA AAACTGGGAA GATGTACCAG 541 GAGACCAGGT CAAGCCCGAC CAATACACTG AGGCCCTGGC CCAGAGGGAC AAGATCTACC 601 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 661 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 721 ATATATCTTG TGGAAAGGAC GATCACCCAG AGTGGGACCC CTTAACACGC GTTAAGTCga 781 caatcaacct ctggattaca aaatttgtga aagatt