Construct: ORF TRCN0000473659
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018917.2_s317c1
- Derived from:
- ccsbBroadEn_15049
- DNA Barcode:
- TATGTCACATGTATTCCAATTGAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- HIPK2 (28996)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473659
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28996 | HIPK2 | homeodomain interacting pro... | NM_001113239.3 | 66.4% | 23.5% | (many diffs) |
| 2 | human | 28996 | HIPK2 | homeodomain interacting pro... | XM_006715935.2 | 65.3% | 23.1% | (many diffs) |
| 3 | human | 28996 | HIPK2 | homeodomain interacting pro... | XM_017012069.1 | 65.3% | 23.1% | (many diffs) |
| 4 | human | 28996 | HIPK2 | homeodomain interacting pro... | NM_022740.5 | 64.9% | 22.9% | (many diffs) |
| 5 | human | 28996 | HIPK2 | homeodomain interacting pro... | XM_011516081.2 | 64.5% | 22.8% | (many diffs) |
| 6 | human | 28996 | HIPK2 | homeodomain interacting pro... | XM_011516079.3 | 57.6% | 20.4% | (many diffs) |
| 7 | human | 28996 | HIPK2 | homeodomain interacting pro... | XM_011516080.3 | 57.5% | 20.6% | (many diffs) |
| 8 | human | 28996 | HIPK2 | homeodomain interacting pro... | XM_011516077.3 | 56.5% | 20% | (many diffs) |
| 9 | human | 28996 | HIPK2 | homeodomain interacting pro... | XM_011516078.3 | 56.4% | 20.2% | (many diffs) |
| 10 | mouse | 15258 | Hipk2 | homeodomain interacting pro... | NM_001136065.2 | 60.1% | 23.3% | (many diffs) |
| 11 | mouse | 15258 | Hipk2 | homeodomain interacting pro... | NM_001294143.1 | 59.8% | 23.2% | (many diffs) |
| 12 | mouse | 15258 | Hipk2 | homeodomain interacting pro... | NM_001294144.1 | 59.7% | 23.1% | (many diffs) |
| 13 | mouse | 15258 | Hipk2 | homeodomain interacting pro... | XM_006505603.3 | 58.8% | 22.9% | (many diffs) |
| 14 | mouse | 15258 | Hipk2 | homeodomain interacting pro... | NM_010433.2 | 58.5% | 22.7% | (many diffs) |
| 15 | mouse | 15258 | Hipk2 | homeodomain interacting pro... | XM_006505602.3 | 58.4% | 22.6% | (many diffs) |
| 16 | mouse | 15258 | Hipk2 | homeodomain interacting pro... | XR_001785097.1 | 14.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1125
- ORF length:
- 1056
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggtccctg ggctgtgtta ttgcagaatt gttcctgggt tggccgttat 121 atccaggagc ttcggagtat gatcagattc ggtatatttc acaaacacag ggtttgcctg 181 ctgaatattt attaagcgcc gggacaaaga caactaggtt tttcaaccgt gacacggact 241 caccatatcc tttgtggaga ctgaagacac cagatgacca tgaagcagag acagggatta 301 agtcaaaaga agcaagaaag tacattttca actgtttaga tgatatggcc caggtgaaca 361 tgacgacaga tttggaaggg agcgacatgt tggtagaaaa ggctgaccgg cgggagttca 421 ttgacctgtt gaagaagatg ctgaccattg atgctgacaa gagaatcact ccaatcgaaa 481 ccctgaacca tccctttgtc accatgacac acttactcga ttttccccac agcacacacg 541 tcaaatcatg tttccagaac atggagatct gcaagcgtcg ggtgaatatg tatgacacgg 601 tgaaccagag caaaacccct ttcatcacgc acgtggcccc cagcacgtcc accaacctga 661 ccatgacctt taacaaccag ctgaccactg tccacaacca gccctcagcg gcatccatgg 721 ctgcagtggc ccagcggagc atgcccctgc agacaggaac agcccagatt tgtgcccggc 781 ctgacccgtt ccagcaagct ctcatcgtgt gtccccccgg cttccaaagg cttgcaggcc 841 tcctcccttc taagcacgct gggctacttc gtgcgaatgg aaaatgcagt tcccatcgtc 901 actcaagccc caggagctca gcctcttcag atccaaccag gtctgcttgc ccagcaggct 961 tggccaagtg ggacccagca gatcctgctt cccccagcat ggcagcaact gactggagtg 1021 gccacccaca catcagtgca gcatgccacc gtgattcccg agaccatggc aggcacccag 1081 cagctggcgg actggagaaa tacgcatgct cacggaagcc attataatcc catcatgcag 1141 cagcctgcac tattgaccgg tcatgtgacc cttccagcag cacagccctt aaatgtgggt 1201 gtggcccacg tgatgcggca gcagccaacc agcaccacct cctcccggaa gagtaagcag 1261 caccagtcat ctgtgagaaa tgtctccacc tgtgaggtgt cctcctctca ggccatcagc 1321 tccccacagc gatccaagcg tgtcaaggag aacacacctc cccgctgtgc catggtgcac 1381 agtagcccgg cctgcagcac ctcggtcacc tgtgggtggg gcgacgtggc ctccagcacc 1441 acccgggaac ggcagcggca gacaattgtc attcccgaca ctcccagccc cacggtcagc 1501 gtcatcacca tcagcagtga cacggacgag gaggaggaac agaaacacgc ccccaccagc 1561 actgtctcca agcaaagaaa aaacgtcatc agctgtgtca cagtccacga ctccccctac 1621 tccgactcct ccagcaacac cagccccctt aatccgtgcc cggcgggggg gggtgggccc 1681 caaaaaaaac caatcccttt gacaccaaag gggacccagg agaatcccag cacggggaaa 1741 cccccgaacc ttctatgtgc cacccctgaa aacccaggcc agcgaagtat tggtggagag 1801 tgatagcctg gtgccagtca acaccagtca ccactcgtcc tcctacaagt ccaagtcctc 1861 cagcaacgtg acctccacca gcggtcactc ttcagggagc tcatctggag ccatcaccta 1921 ccggcagcag cggccgggcc cccacttcca gcagcagcag ccactcaatc tcagccaggc 1981 tcagcagcac atcaccacgg accgcactgg gagccaccga aggcagcagg cctacatcac 2041 tcccaccatg gcccaggctc cgtacTCCTT CCCGCACAAC AGCCCCAGCC ACGGCACTGT 2101 GCACCCGCAT CTGGCTGCAG CCGCTGCCGC TGCCCACCTC CCCACCCAGC CCCACCTCTA 2161 CACCTACACT GCGCCGGCGG CCCTGGGCTC CACCGGCACC GTGGCCCACC TGGTGGCCTC 2221 GCAAGGCTCT GCGCGCCACA CCGTGCAGCA CACTGCCTAC CCAGCCAGCA TCGTCCACCA 2281 GGTCCCCGTG AGCATGGGCC CCCGGGTCCT GCCCTCGCCC ACCATCCACC CGAGTCAGTA 2341 TCCAGCCCAA TTTGCCCACC AGACCTACAT CAGCGCCTCG CCAGCCTCCA CCGTCTACAC 2401 TGGATACCCA CTGAGCCCCG CCAAGGTCAA CCAGTACCCT TACATATTGC CAACTTTCTT 2461 GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 2521 GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 2581 TGGAAAGGAC GATATGTCAC ATGTATTCCA ATTGACACGC GTTAAGTCga caatcaacct 2641 ctggattaca aaatttgtga aagatt