Construct: ORF TRCN0000473696
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003795.1_s317c1
- Derived from:
- ccsbBroadEn_02047
- DNA Barcode:
- CCATGTTTTGTACAACTTATCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP1S2 (8905)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473696
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8905 | AP1S2 | adaptor related protein com... | NM_003916.5 | 100% | 100% | |
| 2 | human | 8905 | AP1S2 | adaptor related protein com... | NM_001272071.2 | 98.1% | 98.1% | 427_435delGATGCGAAA |
| 3 | human | 8905 | AP1S2 | adaptor related protein com... | NM_001369007.1 | 93.1% | 91.2% | (many diffs) |
| 4 | human | 8905 | AP1S2 | adaptor related protein com... | NM_001369008.1 | 92.9% | 90.4% | (many diffs) |
| 5 | human | 8905 | AP1S2 | adaptor related protein com... | NM_001368994.1 | 92.3% | 91.7% | (many diffs) |
| 6 | human | 8905 | AP1S2 | adaptor related protein com... | XM_017029925.1 | 73.9% | 72.2% | (many diffs) |
| 7 | human | 8905 | AP1S2 | adaptor related protein com... | XM_017029926.2 | 73.3% | 71.3% | (many diffs) |
| 8 | human | 8905 | AP1S2 | adaptor related protein com... | XM_005274614.3 | 73.1% | 72.3% | (many diffs) |
| 9 | human | 653653 | LOC653653 | adaptor related protein com... | NR_027408.1 | 39.3% | (many diffs) | |
| 10 | human | 8905 | AP1S2 | adaptor related protein com... | NR_160933.1 | 22.1% | (many diffs) | |
| 11 | human | 8905 | AP1S2 | adaptor related protein com... | XR_002958809.1 | 22% | 1_50del;476_570del;617_2136del | |
| 12 | human | 8905 | AP1S2 | adaptor related protein com... | NR_160932.1 | 21.1% | (many diffs) | |
| 13 | human | 8905 | AP1S2 | adaptor related protein com... | XR_247289.3 | 19.9% | 1_279del;705_799del;846_2365del | |
| 14 | human | 8905 | AP1S2 | adaptor related protein com... | XR_001755741.2 | 12.1% | (many diffs) | |
| 15 | mouse | 108012 | Ap1s2 | adaptor-related protein com... | NM_001290378.1 | 94.9% | 100% | (many diffs) |
| 16 | mouse | 108012 | Ap1s2 | adaptor-related protein com... | NM_001290379.1 | 93.1% | 98.1% | (many diffs) |
| 17 | mouse | 108012 | Ap1s2 | adaptor-related protein com... | XM_011247769.2 | 93.1% | 98.1% | (many diffs) |
| 18 | mouse | 108012 | Ap1s2 | adaptor-related protein com... | NM_026887.3 | 88.4% | 91.2% | (many diffs) |
| 19 | mouse | 108012 | Ap1s2 | adaptor-related protein com... | XM_006528678.3 | 86.7% | 91.7% | (many diffs) |
| 20 | mouse | 108012 | Ap1s2 | adaptor-related protein com... | XR_387077.3 | 28.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 537
- ORF length:
- 471
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gtttatgttg ctttttagtc gtcagggaaa gcttcgactg caaaaatggt 121 atgTCCCACT ATCAGACAAA GAGAAGAAAA AGATCACAAG AGAACTTGTT CAGACCGTTT 181 TAGCACGGAA ACCTAAAATG TGCAGCTTCC TTGAGTGGCG AGATCTGAAG ATTGTTTACA 241 AAAGATATGC TAGTCTGTAT TTTTGCTGTG CTATTGAGGA TCAGGACAAT GAACTAATTA 301 CCCTGGAAAT AATTCATCGT TATGTGGAAT TACTTGACAA GTATTTCGGC AGTGTCTGTG 361 AACTAGATAT CATCTTTAAT TTTGAGAAGG CTTATTTTAT TTTGGATGAG TTTCTTTTGG 421 GAGGGGAAGT TCAGGAAACA TCCAAGAAAA ATGTCCTTAA AGCAATTGAG CAGGCTGATC 481 TACTGCAGGA GGAAGCTGAA ACCCCACGTA GTGTTCTTGA AGAAATTGGA CTGACATACC 541 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GACCATGTTT TGTACAACTT ATCTTTACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt