Construct: ORF TRCN0000473698

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013522.1_s317c1
Derived from:
ccsbBroadEn_09670
DNA Barcode:
TTACTTGTTCAATCGAACGCGGTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CBWD2 (150472)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473698
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 150472 CBWD2 COBW domain containing 2 NM_172003.3 99.8% 99.7% 646T>C;724G>A
2 human 55871 CBWD1 COBW domain containing 1 NM_018491.3 98.8% 98.2% (many diffs)
3 human 445571 CBWD3 COBW domain containing 3 NM_201453.3 98.6% 97.7% (many diffs)
4 human 220869 CBWD5 COBW domain containing 5 NM_001330668.1 98.6% 97.4% (many diffs)
5 human 644019 CBWD6 COBW domain containing 6 NM_001085457.1 98.2% 96.9% (many diffs)
6 human 150472 CBWD2 COBW domain containing 2 NM_001330336.1 95.1% 95.1% 646T>C;704_705ins57
7 human 55871 CBWD1 COBW domain containing 1 NM_001145356.1 94% 93.6% (many diffs)
8 human 55871 CBWD1 COBW domain containing 1 XM_005251513.4 93.9% 93.1% (many diffs)
9 human 445571 CBWD3 COBW domain containing 3 XM_005277637.2 93.9% 93.1% (many diffs)
10 human 220869 CBWD5 COBW domain containing 5 NM_001363751.1 93.9% 92.9% (many diffs)
11 human 220869 CBWD5 COBW domain containing 5 XM_005272750.1 93.7% 92.6% (many diffs)
12 human 445571 CBWD3 COBW domain containing 3 NM_001291821.1 93.6% 92.6% (many diffs)
13 human 644019 CBWD6 COBW domain containing 6 XM_017015035.1 93.5% 92.4% (many diffs)
14 human 644019 CBWD6 COBW domain containing 6 XM_017015020.1 93.3% 91.8% (many diffs)
15 human 150472 CBWD2 COBW domain containing 2 NM_001330337.1 92.5% 92.4% 572_573ins87;637G>A
16 human 55871 CBWD1 COBW domain containing 1 XM_005251514.1 91.8% 91.1% (many diffs)
17 human 445571 CBWD3 COBW domain containing 3 XM_005277639.2 91.6% 90.8% (many diffs)
18 human 220869 CBWD5 COBW domain containing 5 XM_005272751.1 91.6% 90.6% (many diffs)
19 human 644019 CBWD6 COBW domain containing 6 XM_024447653.1 91.3% 90.3% (many diffs)
20 human 644019 CBWD6 COBW domain containing 6 XM_017015019.1 89.9% 88.5% (many diffs)
21 human 150472 CBWD2 COBW domain containing 2 NM_001330339.1 88.6% 86.5% (many diffs)
22 human 644019 CBWD6 COBW domain containing 6 XM_017015023.1 88.6% 87.3% (many diffs)
23 human 150472 CBWD2 COBW domain containing 2 XM_005263604.2 87.8% 87.8% 572_573ins87;617_618ins57
24 human 55871 CBWD1 COBW domain containing 1 NM_001145355.1 87.7% 85.2% (many diffs)
25 human 55871 CBWD1 COBW domain containing 1 XM_005251515.1 87% 86.5% (many diffs)
26 human 445571 CBWD3 COBW domain containing 3 XM_005277640.2 86.9% 86.3% (many diffs)
27 human 220869 CBWD5 COBW domain containing 5 NM_001024916.3 86.9% 86% (many diffs)
28 human 445571 CBWD3 COBW domain containing 3 XM_017014730.1 86.6% 85.8% (many diffs)
29 human 644019 CBWD6 COBW domain containing 6 XM_017015036.1 86.6% 85.8% (many diffs)
30 human 644019 CBWD6 COBW domain containing 6 XM_017015024.1 86.4% 85.3% (many diffs)
31 human 644019 CBWD6 COBW domain containing 6 XM_017015021.1 85.3% 84.1% (many diffs)
32 human 644019 CBWD6 COBW domain containing 6 XM_017015022.1 83.3% 82.1% (many diffs)
33 human 55871 CBWD1 COBW domain containing 1 XM_011517961.2 83% 80.7% (many diffs)
34 human 150472 CBWD2 COBW domain containing 2 XM_011510667.1 80.1% 80% (many diffs)
35 human 55871 CBWD1 COBW domain containing 1 XM_011517962.3 79.3% 78.9% (many diffs)
36 human 445571 CBWD3 COBW domain containing 3 XM_006717116.1 79% 78.2% (many diffs)
37 human 220869 CBWD5 COBW domain containing 5 XM_006716914.1 79% 77.9% (many diffs)
38 human 644019 CBWD6 COBW domain containing 6 XM_017015025.1 78.7% 77.8% (many diffs)
39 human 445571 CBWD3 COBW domain containing 3 XR_001746301.1 72.2% (many diffs)
40 human 220869 CBWD5 COBW domain containing 5 XM_017014455.1 72% 71.1% (many diffs)
41 human 644019 CBWD6 COBW domain containing 6 XM_017015026.1 71.1% 70% (many diffs)
42 human 445571 CBWD3 COBW domain containing 3 XR_001746303.1 68.8% (many diffs)
43 human 220869 CBWD5 COBW domain containing 5 XM_017014456.1 67.8% 67% (many diffs)
44 human 220869 CBWD5 COBW domain containing 5 XM_024447453.1 67.8% 67% (many diffs)
45 human 644019 CBWD6 COBW domain containing 6 XM_017015027.1 67% 61.1% (many diffs)
46 human 445571 CBWD3 COBW domain containing 3 XR_001746302.1 66.5% (many diffs)
47 human 220869 CBWD5 COBW domain containing 5 XR_001746234.1 64.9% (many diffs)
48 human 220869 CBWD5 COBW domain containing 5 XR_428504.1 64.8% (many diffs)
49 human 150472 CBWD2 COBW domain containing 2 XM_024452717.1 63.9% 64% 0_1ins369;277T>C;335_336ins57
50 human 55871 CBWD1 COBW domain containing 1 XR_001746352.1 63.5% (many diffs)
51 human 220869 CBWD5 COBW domain containing 5 XR_001746232.1 63.3% (many diffs)
52 human 445571 CBWD3 COBW domain containing 3 XR_001746299.1 62.6% (many diffs)
53 human 644019 CBWD6 COBW domain containing 6 XM_017015030.1 62.3% 56.7% (many diffs)
54 human 150472 CBWD2 COBW domain containing 2 NR_138473.1 61.9% (many diffs)
55 human 220869 CBWD5 COBW domain containing 5 XR_002956769.1 61.6% (many diffs)
56 human 55871 CBWD1 COBW domain containing 1 XM_011517963.1 61.6% 61.2% (many diffs)
57 human 445571 CBWD3 COBW domain containing 3 XR_001746297.1 61.4% (many diffs)
58 human 220869 CBWD5 COBW domain containing 5 XM_017014457.1 60.8% 60.2% (many diffs)
59 human 644019 CBWD6 COBW domain containing 6 XM_017015028.1 60.2% 59.2% (many diffs)
60 human 220869 CBWD5 COBW domain containing 5 XR_001746237.2 60.1% (many diffs)
61 human 55871 CBWD1 COBW domain containing 1 XM_005251517.5 59.8% 58.5% (many diffs)
62 human 644019 CBWD6 COBW domain containing 6 XM_017015029.2 59.1% 58.2% (many diffs)
63 human 150472 CBWD2 COBW domain containing 2 NR_138474.1 58.8% (many diffs)
64 human 445571 CBWD3 COBW domain containing 3 XR_001746300.1 58.7% (many diffs)
65 human 55871 CBWD1 COBW domain containing 1 XM_017014925.1 58.5% 58.7% (many diffs)
66 human 220869 CBWD5 COBW domain containing 5 XM_017014458.1 58.3% 57.7% (many diffs)
67 human 220869 CBWD5 COBW domain containing 5 XR_428503.2 58.1% (many diffs)
68 human 644019 CBWD6 COBW domain containing 6 XR_001746360.1 58.1% (many diffs)
69 human 220869 CBWD5 COBW domain containing 5 XR_002956768.1 57.6% (many diffs)
70 human 445571 CBWD3 COBW domain containing 3 XR_002956784.1 55.7% (many diffs)
71 human 150472 CBWD2 COBW domain containing 2 NM_001330340.1 55.3% 54.6% (many diffs)
72 human 150472 CBWD2 COBW domain containing 2 NM_001330342.1 55.3% 54.6% (many diffs)
73 human 220869 CBWD5 COBW domain containing 5 XM_017014459.1 54.9% 53.6% (many diffs)
74 human 445571 CBWD3 COBW domain containing 3 XM_011518706.2 54.8% 53.6% (many diffs)
75 human 644019 CBWD6 COBW domain containing 6 XM_017015032.1 54.5% 52.5% (many diffs)
76 human 644019 CBWD6 COBW domain containing 6 XM_024447652.1 54.5% 52.8% (many diffs)
77 human 445571 CBWD3 COBW domain containing 3 XR_929802.1 54.1% (many diffs)
78 human 150472 CBWD2 COBW domain containing 2 XR_244814.2 53% (many diffs)
79 human 55871 CBWD1 COBW domain containing 1 XM_011517964.3 52.8% 51.5% (many diffs)
80 human 644019 CBWD6 COBW domain containing 6 XM_017015031.1 52.5% 50.9% (many diffs)
81 human 220869 CBWD5 COBW domain containing 5 XR_001746239.1 51.6% (many diffs)
82 human 220869 CBWD5 COBW domain containing 5 XR_001746240.1 50.4% (many diffs)
83 human 644019 CBWD6 COBW domain containing 6 XR_001746359.1 49.8% (many diffs)
84 human 445571 CBWD3 COBW domain containing 3 XR_929803.1 49.8% (many diffs)
85 human 55871 CBWD1 COBW domain containing 1 XR_001746353.1 49.2% (many diffs)
86 human 220869 CBWD5 COBW domain containing 5 XR_001746238.1 48.7% (many diffs)
87 human 150472 CBWD2 COBW domain containing 2 XR_922864.1 48.5% (many diffs)
88 human 445571 CBWD3 COBW domain containing 3 XR_929804.1 48.3% (many diffs)
89 human 220869 CBWD5 COBW domain containing 5 XM_017014461.1 47.9% 46.8% (many diffs)
90 human 445571 CBWD3 COBW domain containing 3 XM_017014732.1 47.9% 46.8% (many diffs)
91 human 150472 CBWD2 COBW domain containing 2 XR_427073.2 47.3% (many diffs)
92 human 445571 CBWD3 COBW domain containing 3 XR_001746298.1 47% (many diffs)
93 human 644019 CBWD6 COBW domain containing 6 XM_017015033.1 46% 44.7% (many diffs)
94 human 644019 CBWD6 COBW domain containing 6 XR_001746361.1 45.9% (many diffs)
95 human 445571 CBWD3 COBW domain containing 3 XR_001746304.1 45.6% (many diffs)
96 human 220869 CBWD5 COBW domain containing 5 XR_001746241.2 44.8% (many diffs)
97 human 644019 CBWD6 COBW domain containing 6 XR_001746362.1 44.2% (many diffs)
98 human 644019 CBWD6 COBW domain containing 6 XR_001746363.1 43% (many diffs)
99 human 220869 CBWD5 COBW domain containing 5 NM_001286835.1 42.7% 39.3% (many diffs)
100 human 150472 CBWD2 COBW domain containing 2 XR_002959242.1 38.1% (many diffs)
101 human 644019 CBWD6 COBW domain containing 6 XM_017015034.1 32% 30.3% (many diffs)
102 human 220869 CBWD5 COBW domain containing 5 NM_001286836.1 28.2% 27.8% (many diffs)
103 human 220869 CBWD5 COBW domain containing 5 XR_002956766.1 26.1% (many diffs)
104 human 220869 CBWD5 COBW domain containing 5 XR_001746233.1 25.8% (many diffs)
105 human 220869 CBWD5 COBW domain containing 5 XR_001746235.1 25.7% (many diffs)
106 human 220869 CBWD5 COBW domain containing 5 XR_001746231.1 25.5% (many diffs)
107 human 220869 CBWD5 COBW domain containing 5 XR_002956767.1 22.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1254
ORF length:
1185
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gttaccggct gttggatctg cggatgagga ggaggatcct gcggaggagg
121attgtcctga attggttccc atggagacga cgcaaagcga ggaggaggaa aagtctggcc
181tcggcgccaa gatcccagtc acaattatca ccgggtattt aggtgctggg aagacaacac
241ttctgaacta tattttgaca gagcaacata gtaaaagagt agcggtcatt ttaaatgaat
301ttggggaagg aagtgcgctg gagaaatcct tagctgtcag ccaaggtgga gagctctatg
361aagagtggct ggaacttaga aacggttgcc tctgctgttc agtgaaggac aatggcctta
421gagctattga gaatttgatg caaaagaagg ggaaatttga ttacatactg ttagagacca
481ctggattggc agacccaggt gcagtggctt ctatgttttg ggttgatgct gaattaggga
541gtgatattta ccttgatggt atcataacta tagtggattc aaaatatgga ttaaaacatt
601tagcagaaga gaaacctgat ggccttatca atgaagctac taggcaagtt gctttggcag
661atgccattct cattaataaa acagacttgg ttccagaaga agatgtaaag aaactaagag
721cgacaattag atccataaat ggactaggac aaaTCTTAGA AACACAAAGA TCAAGAGTTG
781ATCTCTCTAA TATATTAGAT CTTCATGCCT TTGATAGTCT CTCTGGAATA AGTTTGCAGA
841AAAAACTTCA GCATGTGCCA GGAACACAAC CTCACCTTGA TCAGAGTATT GTTACAATCA
901CATTTGAAGT ACCAGGAAAT GCAAAGGAAG AACATCTTAA TATGTTTATT CAGAATCTCC
961TGTGGGAAAA GAATGTGAGA AACAAGGACA ATCACTGCAT GGAGGTCATA AGGCTGAAGG
1021GATTGGTGTC AATCAAAGAC AAATCACAAC AAGTGATTGT CCAGGGTGTC CATGAGCTCT
1081ATGATCTGGA GGAGACTCCA GTGAGCTGGA AGGATGACAC TGAGAGAACA AATCGATTGG
1141TCCTCCTTGG CAGAAATTTA GATAAGGATA TCCTTAAACA GCTGTTTATA GCTACTGTGA
1201CAGAAACAGA AAAGCAGTGG ACAACACATT TCAAAGAAGA TCAAGTTTGT ACATTGCCAA
1261CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1321ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1381TATCTTGTGG AAAGGACGAT TACTTGTTCA ATCGAACGCG GTTACGCGTT AAGTCgacaa
1441tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)