Transcript: Human NM_001286836.1

Homo sapiens COBW domain containing 5 (CBWD5), transcript variant 3, mRNA.

Source:
NCBI, updated 2018-09-16
Taxon:
Homo sapiens (human)
Gene:
CBWD5 (220869)
Length:
655
CDS:
144..485

Additional Resources:

NCBI RefSeq record:
NM_001286836.1
NBCI Gene record:
CBWD5 (220869)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001286836.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000242508 ACAACACTTCTGAACTATATT pLKO_005 309 CDS 100% 15.000 7.500 Y CBWD1 n/a
2 TRCN0000242509 ACAATTATCACCGGGTATTTA pLKO_005 276 CDS 100% 15.000 7.500 Y CBWD1 n/a
3 TRCN0000426044 CAACACTTCTGAACTATATTT pLKO_005 310 CDS 100% 15.000 7.500 Y CBWD2 n/a
4 TRCN0000262469 CACAATTATCACCGGGTATTT pLKO_005 275 CDS 100% 13.200 6.600 Y CBWD6 n/a
5 TRCN0000130195 CCAAGATCCCAGTCACAATTA pLKO.1 262 CDS 100% 13.200 6.600 Y CBWD2 n/a
6 TRCN0000135615 GTCACAATTATCACCGGGTAT pLKO.1 273 CDS 100% 4.050 2.025 Y CBWD3 n/a
7 TRCN0000128085 CTCTATGAAGAGTGGCTGGAA pLKO.1 429 CDS 100% 2.640 1.320 Y CBWD2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001286836.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13415 pDONR223 100% 62.7% 62.7% None 339_340ins201 n/a
2 ccsbBroad304_13415 pLX_304 0% 62.7% 62.7% V5 339_340ins201 n/a
3 TRCN0000480607 CTTTTTTTTCAGTAGCCCCGCCGA pLX_317 73.5% 62.7% 62.7% V5 339_340ins201 n/a
4 ccsbBroadEn_15265 pDONR223 73.3% 28.1% 27.5% None (many diffs) n/a
5 ccsbBroad304_15265 pLX_304 0% 28.1% 27.5% V5 (many diffs) n/a
6 ccsbBroadEn_10172 pDONR223 100% 28.6% 28.6% None 339_340ins846 n/a
7 ccsbBroad304_10172 pLX_304 0% 28.6% 28.6% V5 339_340ins846 n/a
8 ccsbBroadEn_08604 pDONR223 100% 28.4% 28.3% None 51G>A;233C>T;339_340ins846 n/a
9 ccsbBroad304_08604 pLX_304 0% 28.4% 28.3% V5 51G>A;233C>T;339_340ins846 n/a
10 ccsbBroadEn_03674 pDONR223 100% 28.3% 28.1% None (many diffs) n/a
11 ccsbBroad304_03674 pLX_304 0% 28.3% 28.1% V5 (many diffs) n/a
12 TRCN0000492257 CAGAGTAATCATATCCCTTTGTTC pLX_317 36.1% 28.3% 28.1% V5 (many diffs) n/a
13 ccsbBroadEn_09670 pDONR223 100% 28.2% 27.8% None (many diffs) n/a
14 TRCN0000473698 TTACTTGTTCAATCGAACGCGGTT pLX_317 42.3% 28.2% 27.8% V5 (many diffs) n/a
15 ccsbBroadEn_08603 pDONR223 100% 17.9% 16% None (many diffs) n/a
16 ccsbBroad304_08603 pLX_304 0% 17.9% 16% V5 (many diffs) n/a
17 TRCN0000491579 GTTCACCCGAGATAGGATGAAGAC pLX_317 32.7% 17.9% 16% V5 (many diffs) n/a
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