Transcript: Human XM_005251517.5

PREDICTED: Homo sapiens COBW domain containing 1 (CBWD1), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CBWD1 (55871)
Length:
1167
CDS:
107..832

Additional Resources:

NCBI RefSeq record:
XM_005251517.5
NBCI Gene record:
CBWD1 (55871)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005251517.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000242508 ACAACACTTCTGAACTATATT pLKO_005 272 CDS 100% 15.000 7.500 Y CBWD1 n/a
2 TRCN0000242509 ACAATTATCACCGGGTATTTA pLKO_005 239 CDS 100% 15.000 7.500 Y CBWD1 n/a
3 TRCN0000426044 CAACACTTCTGAACTATATTT pLKO_005 273 CDS 100% 15.000 7.500 Y CBWD2 n/a
4 TRCN0000242510 CTGAATTAGGGAGTGATATTT pLKO_005 567 CDS 100% 15.000 7.500 Y CBWD1 n/a
5 TRCN0000427377 GCAGATGCCATTCTCATTAAT pLKO_005 695 CDS 100% 15.000 7.500 Y CBWD2 n/a
6 TRCN0000262469 CACAATTATCACCGGGTATTT pLKO_005 238 CDS 100% 13.200 6.600 Y CBWD6 n/a
7 TRCN0000130195 CCAAGATCCCAGTCACAATTA pLKO.1 225 CDS 100% 13.200 6.600 Y CBWD2 n/a
8 TRCN0000242511 CTTGATGGTATCATAACTATT pLKO_005 590 CDS 100% 13.200 6.600 Y CBWD1 n/a
9 TRCN0000262471 TTGATGGTATCATAACTATTG pLKO_005 591 CDS 100% 10.800 5.400 Y CBWD6 n/a
10 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 1133 3UTR 100% 4.950 2.475 Y ERAP2 n/a
11 TRCN0000135519 GATGCTGAATTAGGGAGTGAT pLKO.1 563 CDS 100% 4.950 2.475 Y CBWD3 n/a
12 TRCN0000134746 GCCTTATCAATGAAGCTACTA pLKO.1 660 CDS 100% 4.950 2.475 Y CBWD3 n/a
13 TRCN0000135615 GTCACAATTATCACCGGGTAT pLKO.1 236 CDS 100% 4.050 2.025 Y CBWD3 n/a
14 TRCN0000128085 CTCTATGAAGAGTGGCTGGAA pLKO.1 392 CDS 100% 2.640 1.320 Y CBWD2 n/a
15 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 1134 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005251517.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13415 pDONR223 100% 73.3% 70.4% None (many diffs) n/a
2 ccsbBroad304_13415 pLX_304 0% 73.3% 70.4% V5 (many diffs) n/a
3 TRCN0000480607 CTTTTTTTTCAGTAGCCCCGCCGA pLX_317 73.5% 73.3% 70.4% V5 (many diffs) n/a
4 ccsbBroadEn_15265 pDONR223 73.3% 59.6% 58.3% None (many diffs) n/a
5 ccsbBroad304_15265 pLX_304 0% 59.6% 58.3% V5 (many diffs) n/a
6 ccsbBroadEn_03674 pDONR223 100% 60.6% 59.5% None (many diffs) n/a
7 ccsbBroad304_03674 pLX_304 0% 60.6% 59.5% V5 (many diffs) n/a
8 TRCN0000492257 CAGAGTAATCATATCCCTTTGTTC pLX_317 36.1% 60.6% 59.5% V5 (many diffs) n/a
9 ccsbBroadEn_08604 pDONR223 100% 60.3% 59.3% None (many diffs) n/a
10 ccsbBroad304_08604 pLX_304 0% 60.3% 59.3% V5 (many diffs) n/a
11 ccsbBroadEn_10172 pDONR223 100% 59.8% 58.3% None (many diffs) n/a
12 ccsbBroad304_10172 pLX_304 0% 59.8% 58.3% V5 (many diffs) n/a
13 ccsbBroadEn_09670 pDONR223 100% 59.8% 58.5% None (many diffs) n/a
14 TRCN0000473698 TTACTTGTTCAATCGAACGCGGTT pLX_317 42.3% 59.8% 58.5% V5 (many diffs) n/a
15 ccsbBroadEn_08603 pDONR223 100% 49.7% 46.8% None (many diffs) n/a
16 ccsbBroad304_08603 pLX_304 0% 49.7% 46.8% V5 (many diffs) n/a
17 TRCN0000491579 GTTCACCCGAGATAGGATGAAGAC pLX_317 32.7% 49.7% 46.8% V5 (many diffs) n/a
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