Construct: ORF TRCN0000473699
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003922.1_s317c1
- Derived from:
- ccsbBroadEn_11268
- DNA Barcode:
- AAATTGTTGTTGTCACATGCCGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H4C8 (8365)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473699
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8365 | H4C8 | H4 clustered histone 8 | NM_003543.3 | 98.3% | 100% | (many diffs) |
| 2 | human | 8367 | H4C5 | H4 clustered histone 5 | NM_003545.3 | 89.6% | 100% | (many diffs) |
| 3 | human | 8360 | H4C4 | H4 clustered histone 4 | NM_003539.3 | 84.4% | 100% | (many diffs) |
| 4 | human | 8362 | H4C12 | H4 clustered histone 12 | NM_003541.2 | 84.1% | 100% | (many diffs) |
| 5 | human | 8363 | H4C11 | H4 clustered histone 11 | NM_021968.3 | 84.1% | 100% | (many diffs) |
| 6 | human | 8294 | H4C9 | H4 clustered histone 9 | NM_003495.2 | 83.8% | 100% | (many diffs) |
| 7 | human | 8361 | H4C6 | H4 clustered histone 6 | NM_003540.3 | 83.8% | 100% | (many diffs) |
| 8 | human | 8366 | H4C2 | H4 clustered histone 2 | NM_003544.2 | 83.4% | 100% | (many diffs) |
| 9 | human | 554313 | H4C15 | H4 clustered histone 15 | NM_001034077.4 | 83.1% | 100% | (many diffs) |
| 10 | human | 8370 | H4C14 | H4 clustered histone 14 | NM_003548.2 | 83.1% | 100% | (many diffs) |
| 11 | human | 8359 | H4C1 | H4 clustered histone 1 | NM_003538.3 | 82.3% | 100% | (many diffs) |
| 12 | human | 8364 | H4C3 | H4 clustered histone 3 | NM_003542.3 | 81.8% | 100% | (many diffs) |
| 13 | human | 8368 | H4C13 | H4 clustered histone 13 | NM_003546.2 | 80.9% | 100% | (many diffs) |
| 14 | human | 121504 | H4-16 | H4 histone 16 | NM_175054.2 | 80.5% | 100% | (many diffs) |
| 15 | mouse | 326619 | Hist1h4a | histone cluster 1, H4a | NM_178192.2 | 83.8% | 100% | (many diffs) |
| 16 | mouse | 326620 | Hist1h4b | histone cluster 1, H4b | NM_178193.2 | 83.8% | 100% | (many diffs) |
| 17 | mouse | 100041230 | Hist1h4m | histone cluster 1, H4m | NM_001195421.1 | 83.4% | 100% | (many diffs) |
| 18 | mouse | 319156 | Hist1h4d | histone cluster 1, H4d | NM_175654.2 | 83.4% | 100% | (many diffs) |
| 19 | mouse | 319161 | Hist1h4n | histone cluster 1, H4n | NM_175657.2 | 83.4% | 100% | (many diffs) |
| 20 | mouse | 97122 | Hist2h4 | histone cluster 2, H4 | NM_033596.3 | 83.1% | 100% | (many diffs) |
| 21 | mouse | 319155 | Hist1h4c | histone cluster 1, H4c | NM_178208.2 | 82.9% | 100% | (many diffs) |
| 22 | mouse | 319159 | Hist1h4j | histone cluster 1, H4j | NM_178210.2 | 82.8% | 100% | (many diffs) |
| 23 | mouse | 319160 | Hist1h4k | histone cluster 1, H4k | NM_178211.2 | 82.8% | 100% | (many diffs) |
| 24 | mouse | 319157 | Hist1h4f | histone cluster 1, H4f | NM_175655.2 | 82.5% | 100% | (many diffs) |
| 25 | mouse | 319158 | Hist1h4i | histone cluster 1, H4i | NM_175656.3 | 82.5% | 100% | (many diffs) |
| 26 | mouse | 69386 | Hist1h4h | histone cluster 1, H4h | NM_153173.4 | 82.2% | 100% | (many diffs) |
| 27 | mouse | 320332 | Hist4h4 | histone cluster 4, H4 | NM_175652.3 | 81.5% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 226
- ORF end:
- 535
- ORF length:
- 309
- Sequence:
-
1 GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC 61 CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT 121 TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG 181 GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCACCATGTC TGGCCGCGGC 241 AAAGGTGGAA AAGGTTTGGG TAAGGGAGGG GCTAAGCGTC ATCGCAAGGT TTTGCGCGAT 301 AACATCCAGG GCATCACTAA GCCAGCTATC CGGCGCCTTG CTCGTCGCGG CGGTGTCAAG 361 CGAATTTCTG GCCTTATCTA TGAGGAGACT CGCGGTGTTC TGAAGGTGTT CCTGGAGAAC 421 GTGATTCGTG ACGCTGTCAC TTACACAGAA CACGCCAAAC GCAAGACCGT GACAGCAATG 481 GATGTGGTCT ACGCGCTGAA GCGACAGGGA CGCACTCTTT ACGGCTTCGG TGGCTTGCCA 541 ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC 601 GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT 661 ATATCTTGTG GAAAGGACGA AAATTGTTGT TGTCACATGC CGTAACGCGT TAAGTCgaca 721 atcaacctct ggattacaaa atttgtgaaa gatt