Construct: ORF TRCN0000473792
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001545.4_s317c1
- Derived from:
- ccsbBroadEn_14783
- DNA Barcode:
- TGATCGGGTGAATCCCGAGTAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKAG1 (5571)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473792
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5571 | PRKAG1 | protein kinase AMP-activate... | NM_002733.5 | 100% | 100% | |
| 2 | human | 5571 | PRKAG1 | protein kinase AMP-activate... | NM_001206709.2 | 97.3% | 97.3% | 250_276del |
| 3 | human | 5571 | PRKAG1 | protein kinase AMP-activate... | NM_001206710.2 | 90.3% | 90.3% | 0_1ins96 |
| 4 | human | 5571 | PRKAG1 | protein kinase AMP-activate... | XM_011538562.2 | 90.3% | 90.3% | 0_1ins96 |
| 5 | human | 5571 | PRKAG1 | protein kinase AMP-activate... | XM_005269020.2 | 74.6% | 74.6% | 0_1ins252 |
| 6 | human | 5571 | PRKAG1 | protein kinase AMP-activate... | XM_017019622.1 | 74.6% | 74.6% | 0_1ins252 |
| 7 | human | 5571 | PRKAG1 | protein kinase AMP-activate... | XM_017019623.1 | 74.6% | 74.6% | 0_1ins252 |
| 8 | human | 5571 | PRKAG1 | protein kinase AMP-activate... | XM_005269019.4 | 72.7% | 76.6% | 1_221del;277_278ins110 |
| 9 | mouse | 19082 | Prkag1 | protein kinase, AMP-activat... | NM_016781.2 | 87.5% | 97.2% | (many diffs) |
| 10 | mouse | 19082 | Prkag1 | protein kinase, AMP-activat... | XM_011245520.1 | 79.6% | 89.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1059
- ORF length:
- 993
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gacggtcatt tcttcagata gctccccagc tgtggaaaat gagcatcctc 121 aagagacccc agaatccaac aatagcgtgt atacttcctt catgaagtct catcgctgct 181 atgacctgat tcccacaagc tccaaattgg ttgtatttga tacgtccctg caggtgaaga 241 aagctttttt tgctttggtg actaacggtg tacgagctgc ccctttatgg gatagtaaga 301 agcaaagttt tgtgggcatg ctgaccatca ctgatttcat caatatcctg caccgctact 361 ataaatcagc cttggtacag atctatgagc tagaagaaca caagatagaa acttggagag 421 aggtgtatct ccaggactcc tttaaaccgc ttgtctgcat ttctcctaat gccagcttgt 481 ttgatgctgt ctcttcatta attcggaaca agatccacag gctgccagtt attgacccag 541 aatcaggCAA TACTTTGTAC ATCCTCACCC ACAAGCGCAT TCTGAAGTTC CTCAAATTGT 601 TTATCACTGA GTTCCCCAAG CCAGAGTTCA TGTCCAAGTC TCTGGAAGAG CTACAGATTG 661 GCACCTATGC CAATATTGCT ATGGTTCGCA CTACCACCCC CGTCTATGTG GCTCTGGGGA 721 TTTTTGTACA GCATCGAGTC TCAGCCCTGC CAGTGGTGGA TGAGAAGGGG CGTGTGGTGG 781 ACATCTACTC CAAGTTTGAT GTTATCAATC TGGCAGCAGA AAAGACCTAC AACAACCTAG 841 ATGTATCTGT GACTAAAGCC TTGCAACATC GATCACATTA CTTTGAGGGT GTTCTCAAGT 901 GCTACCTGCA TGAGACTCTG GAGACCATCA TCAACAGGCT AGTGGAAGCA GAGGTTCACC 961 GACTTGTAGT GGTGGATGAA AATGATGTGG TCAAGGGAAT TGTATCACTG TCTGACATCC 1021 TGCAGGCCCT GGTGCTCACA GGTGGAGAGA AGAAGCCCTG CCCAACTTTC TTGTACAAAG 1081 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1141 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1201 ACGATGATCG GGTGAATCCC GAGTAAAGAC GCGTTAAGTC gacaatcaac ctctggatta 1261 caaaatttgt gaaagatt