Construct: ORF TRCN0000473824
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009717.1_s317c1
- Derived from:
- ccsbBroadEn_11599
- DNA Barcode:
- GGGCTTCATTACTAATACTCACGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CLASRP (11129)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473824
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11129 | CLASRP | CLK4 associating serine/arg... | NM_007056.3 | 29.8% | 11.3% | (many diffs) |
| 2 | human | 11129 | CLASRP | CLK4 associating serine/arg... | XM_011526396.2 | 28.7% | 10.9% | (many diffs) |
| 3 | human | 11129 | CLASRP | CLK4 associating serine/arg... | XR_002958247.1 | 27.9% | (many diffs) | |
| 4 | human | 11129 | CLASRP | CLK4 associating serine/arg... | NR_103529.2 | 26.9% | (many diffs) | |
| 5 | mouse | 53609 | Clasrp | CLK4-associating serine/arg... | NM_016680.5 | 26.9% | 11.4% | (many diffs) |
| 6 | mouse | 53609 | Clasrp | CLK4-associating serine/arg... | XM_011250629.1 | 26.9% | 11.4% | (many diffs) |
| 7 | mouse | 53609 | Clasrp | CLK4-associating serine/arg... | XM_006540191.3 | 26.5% | 11.3% | (many diffs) |
| 8 | mouse | 53609 | Clasrp | CLK4-associating serine/arg... | XM_017322361.1 | 26.5% | 11.3% | (many diffs) |
| 9 | mouse | 53609 | Clasrp | CLK4-associating serine/arg... | NR_125832.1 | 24.3% | (many diffs) | |
| 10 | mouse | 53609 | Clasrp | CLK4-associating serine/arg... | NR_125835.1 | 22.2% | (many diffs) | |
| 11 | mouse | 53609 | Clasrp | CLK4-associating serine/arg... | NR_125834.1 | 21.9% | (many diffs) | |
| 12 | mouse | 53609 | Clasrp | CLK4-associating serine/arg... | NR_125833.1 | 21.8% | (many diffs) | |
| 13 | mouse | 53609 | Clasrp | CLK4-associating serine/arg... | XM_006540192.1 | 20.7% | 4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 354
- ORF length:
- 288
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt cgactacaag aagagggcgg agcggagacg ggagtattat gaaaagatca 121 agaaggaccc agcccagttc ctgcaggtac atggccgagc ttgcaaggtg cacctggatt 181 ctgcagtcgc cctggccgct gagagccctg ttaatatgat gcccTGGCAG GGGGACACCA 241 ACAACATGAT TGACCGATTC GATGTCCGTG CCCACCTGGA CCACATCCCC CGACTACACC 301 CCCCCTCTGC TCACCACCAT CTCCCCAGAA CAGGAGTCGG ACGAACGGAA GTGTAACTAC 361 GAGCGCTACA GAGGCCTGGT GCAGAACGAC TTTGCCGGCA TCTCAGAGGA GCAGTGCCTG 421 TACCAGATCT ACATTGATGA GTTGTACGGA GGCCTCCAGA GACCCAGCGA AGATGAGAAG 481 AAGAAGCTGG CAGAGAAGAA GGCTTCCATC GGTTATACCT ACGAGGACCT CCAGCTCCCG 541 CTCCAGCTCT CGCTCCAGCT CCCGCTCTCG CCGTGGTGGG GGCTACTACC GTTCCGGCCG 601 CCACGCCCGC TCCCGGTCCC GCTCCTGGTC CCGCTCCCGC TCCCGCTCCC GGCGCTATTC 661 CCGGTCCCGT ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC 721 CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG 781 ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGGCT TCATTACTAA TACTCACGTA 841 CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt