Construct: ORF TRCN0000473837
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002174.1_s317c1
- Derived from:
- ccsbBroadEn_11666
- DNA Barcode:
- TCGCTGTTTCTTTCGAGCCGGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDS5B (23047)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473837
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23047 | PDS5B | PDS5 cohesin associated fac... | XM_017020453.1 | 8.2% | 7.7% | (many diffs) |
| 2 | human | 23047 | PDS5B | PDS5 cohesin associated fac... | XM_017020452.2 | 8.2% | 7.7% | (many diffs) |
| 3 | human | 23047 | PDS5B | PDS5 cohesin associated fac... | XM_011535000.2 | 8% | 7.5% | (many diffs) |
| 4 | human | 23047 | PDS5B | PDS5 cohesin associated fac... | XM_011534999.2 | 8% | 7.5% | (many diffs) |
| 5 | human | 23047 | PDS5B | PDS5 cohesin associated fac... | XM_017020451.1 | 8% | 7.5% | (many diffs) |
| 6 | human | 23047 | PDS5B | PDS5 cohesin associated fac... | NM_015032.4 | 8% | 7.5% | (many diffs) |
| 7 | human | 23047 | PDS5B | PDS5 cohesin associated fac... | XM_017020450.1 | 8% | 7.5% | (many diffs) |
| 8 | human | 23047 | PDS5B | PDS5 cohesin associated fac... | XM_005266298.4 | 8% | 7.5% | (many diffs) |
| 9 | human | 23047 | PDS5B | PDS5 cohesin associated fac... | XM_017020448.1 | 8% | 7.5% | (many diffs) |
| 10 | human | 23047 | PDS5B | PDS5 cohesin associated fac... | XM_017020449.1 | 8% | 7.5% | (many diffs) |
| 11 | mouse | 100710 | Pds5b | PDS5 cohesin associated fac... | XM_006504904.1 | 7.6% | 7.6% | (many diffs) |
| 12 | mouse | 100710 | Pds5b | PDS5 cohesin associated fac... | NM_175310.6 | 7.5% | 7.5% | (many diffs) |
| 13 | mouse | 100710 | Pds5b | PDS5 cohesin associated fac... | XM_006504903.1 | 7.5% | 7.5% | (many diffs) |
| 14 | mouse | 100710 | Pds5b | PDS5 cohesin associated fac... | NM_001346503.1 | 7.5% | 7.5% | (many diffs) |
| 15 | mouse | 100710 | Pds5b | PDS5 cohesin associated fac... | XM_006504901.1 | 7.4% | 7.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 456
- ORF length:
- 366
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCTCATTC AAAGACTAGG ACCAATGATG 121 GAAAAATTAC ATATCCGCCT GGGGTCAAGG AAATATCAGA TAAAATATCT AAAGAGGAGA 181 TGGTGAGACG ATTAAAGATG GTTGTGAAAA CTTTTATGGA TATGGACCAG GACTCTGAAG 241 AAGAAAAGGA GCTTTATTTA AACCTAGCTT TACATCTTGC TTCAGATTTT TTTCTCAAGC 301 ATCCTGATAA AGATGTTCGC TTACTGGTAG CCTGCTGCCT TGCTGATATT TTCAGGATTT 361 ATGCTCCTGA AGCTCCTTAC ACATCCCCTG ATAAACTAAA GGCAAGTACT GATTTAAATA 421 ACTCCAAGAT TGACCGATAC TTTGATTTAT CTTTCTACCC AACTTTCTTG TACAAAGTGG 481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 601 ATCGCTGTTT CTTTCGAGCC GGCCGACGCG TTAAGTCgac aatcaacctc tggattacaa 661 aatttgtgaa agatt