Transcript: Mouse NM_001346503.1

Mus musculus PDS5 cohesin associated factor B (Pds5b), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Pds5b (100710)
Length:
7314
CDS:
147..4493

Additional Resources:

NCBI RefSeq record:
NM_001346503.1
NBCI Gene record:
Pds5b (100710)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001346503.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000191354 CGACATCAAGTAAAGGATTTA pLKO.1 1653 CDS 100% 13.200 18.480 N Pds5b n/a
2 TRCN0000191169 CCCGATAAACTAAAGGATATA pLKO.1 444 CDS 100% 13.200 10.560 N Pds5b n/a
3 TRCN0000191743 GCTCAAGCTATTGAACCATAT pLKO.1 822 CDS 100% 10.800 7.560 N Pds5b n/a
4 TRCN0000022016 CCAGAGTATGTTGTTCCATAT pLKO.1 3108 CDS 100% 10.800 6.480 N PDS5B n/a
5 TRCN0000159276 GAAGAAGAAGAAAGACAAAGA pLKO.1 4128 CDS 100% 4.950 2.475 Y C1orf35 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001346503.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11665 pDONR223 100% 32.6% 33.9% None (many diffs) n/a
2 ccsbBroad304_11665 pLX_304 0% 32.6% 33.9% V5 (many diffs) n/a
3 TRCN0000470658 CTTTTAGTCAGTTTATGCTCCCGG pLX_317 31.8% 32.6% 33.9% V5 (many diffs) n/a
4 ccsbBroadEn_11666 pDONR223 100% 7.5% 7.5% None (many diffs) n/a
5 ccsbBroad304_11666 pLX_304 0% 7.5% 7.5% V5 (many diffs) n/a
6 TRCN0000473837 TCGCTGTTTCTTTCGAGCCGGCCG pLX_317 100% 7.5% 7.5% V5 (many diffs) n/a
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