Construct: ORF TRCN0000473889
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018916.3_s317c1
- Derived from:
- ccsbBroadEn_14609
- DNA Barcode:
- ACCCGTCACCAACAGAGATTCCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DAPK1 (1612)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473889
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1612 | DAPK1 | death associated protein ki... | NM_001288729.1 | 99.3% | 15.8% | (many diffs) |
| 2 | human | 1612 | DAPK1 | death associated protein ki... | NM_001288730.2 | 99.3% | 15.8% | (many diffs) |
| 3 | human | 1612 | DAPK1 | death associated protein ki... | NM_001288731.2 | 99.3% | 15.8% | (many diffs) |
| 4 | human | 1612 | DAPK1 | death associated protein ki... | NM_004938.4 | 99.3% | 15.8% | (many diffs) |
| 5 | human | 1612 | DAPK1 | death associated protein ki... | XM_017014360.1 | 69.9% | 5.2% | (many diffs) |
| 6 | human | 1612 | DAPK1 | death associated protein ki... | XM_005251757.4 | 57.5% | 26.8% | (many diffs) |
| 7 | mouse | 69635 | Dapk1 | death associated protein ki... | NM_029653.3 | 87.1% | 15.8% | (many diffs) |
| 8 | mouse | 69635 | Dapk1 | death associated protein ki... | NM_134062.2 | 87.1% | 15.8% | (many diffs) |
| 9 | mouse | 69635 | Dapk1 | death associated protein ki... | NM_001285917.1 | 86.4% | 15.6% | (many diffs) |
| 10 | mouse | 69635 | Dapk1 | death associated protein ki... | XM_006517370.3 | 86.4% | 15.6% | (many diffs) |
| 11 | mouse | 69635 | Dapk1 | death associated protein ki... | XM_006517371.3 | 85.9% | 14.4% | (many diffs) |
| 12 | mouse | 69635 | Dapk1 | death associated protein ki... | XM_017315598.1 | 85.1% | 14.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 774
- ORF length:
- 705
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccgtgttc aggcaggaaa acgtggatga ttactacgac accggcgagg 121 aacttggcag tggacagttt gcggttgtga agaaatgccg tgagaaaagc accggcctcc 181 agtatgccgc caaattcatc aagaaaagga ggactaagtc cagccggcgg ggtgtgagcc 241 gcgaggacat cgagcgggag gtcagcatcc tgaaggagat ccagcacccc aatgtcatca 301 ccctgcacga ggtctatgag aacaagacgg acgtcatcct gatcttggaa ctcgttgcag 361 gtggcgagct gtttgacttc ttagctgaaa aggaatcttt aactgaagag gaagcaactg 421 aatttctcaa acaaattctt aatggtgttt actacctgca ctcccttcaa atcgcccact 481 ttgatcttaa gcctgagaac ataatgcttt tggatagaaa tgtccccaaa cctcggatca 541 agatcattga ctttgggttg gcccataaaa ttgactttgg aaatgaattt aaaaacatat 601 ttgggactcc agagtttgtc gctcctgaga tagtcaacta tgaacctctt ggtcttgagg 661 cagatatgtg gagtatcggg gtaataacct atatcctcct aagtggggcc tccccatttc 721 ttggagacac taagcaagaa acgttagcaa tgtatccgct gtcaactacg atttgagatg 781 atacttcagt ataccagtgc cctagccaaa gatttcatag agacttctgg tcagatccaa 841 agagagatga cattcagata gttgcagcat ccctggatca agcctaaaga tacacaacag 901 gcacttagta gaaaagcatc agcagtaaac atggagaaat tcaagaagtt tgcagcccgg 961 aaaaaatgga aacaatccgt tcgcttgata tcactgtgcc aaagattatc caggtcattc 1021 ctgtccagaa gtaacatgag tgttgccaga agcgatgata ctctggatga ggaagactcc 1081 tttgtgatga aagccatcat ccatgccatc aacgatgaca atgtcccagg cctgcagcac 1141 cttctgggct cattatccaa ctatgatgtt aaccaaccca acaagcacgg gacacctcca 1201 ttactcattg ctgctggctg tgggaatatt caaatactac agttgctcat taaaagaggc 1261 tcgagaatcg atgtccagga taagggcggg tccaatgccg tctactgggc tgctcggcat 1321 ggccacgtcg ataccttgaa atttctcagt gagaacaaat gccctttgga tgtgaaagac 1381 aagtctggag agatggccct ccacgtggca gctcgctatg gccatgctga cgtggctcag 1441 ttactgtgca gcttcggctc aaatcccaat atccaggaca aggaagaaga aacccccctg 1501 cactgtgctg cttggcacgg ctattactct gtggccaaag ccctttgtga agccggctgt 1561 aacgtgaaca tcaagaaccg agaaggagag acgcccctcc tgacagcctc tgccaggggc 1621 taccacgaca tcgtggagtg tctggccgaa catggagccg accttaatgc ttgcgacaag 1681 gacggacaca ttgcccttca tctggctgta agacggtgtc agatggaggt aatcaagact 1741 ctcctcagcc aagggtgttt cgtcgattat caagacaggc acggcaatac tcccctccat 1801 gtggcatgta aagatggcaa catgcctatc gtggtggccc tctgtgaagc aaactgcaat 1861 ttggacatct ccaacaagta tgggcgaacg cctctgcacc ttgcggccaa caacggaatc 1921 ctagacgtgg tccggtatct ctgtctgatg ggagccagcg ttgaggcgct gaccacggac 1981 ggaaagacgg cagaagatct tgctagatcg gaacagcacg agcacgtagc aggtctcctt 2041 gcaagacttc gaaaggatac gcaccgagga ctcttcatcc agcagctccg acccacacag 2101 aacctgcagc caagaattaa gctcaagctg tttggccact cgggatccgg gaaaaccacc 2161 cttgtagaat ctctcaagtg tgggctgctg aggagctttt tcagaaggcg tcggcccaga 2221 ctgtcttcca ccaactccag caggttccca ccttcacccc tggcttctaa gcccacagtc 2281 tcagtgagca tcaacaacct gtacccaggc tgcgagaacg tgagtgtgag gagccgcagc 2341 atgatgttcg agccgggtct taccaaaggg atgctggagg tgtttgtggc cccgacccac 2401 cacccgcact gctcggccga tgaccagtcc accaaggcca tcgacatcca gaacgcttat 2461 ttgaatggag ttggcgattt cagcgtgtgg gagttctctg gaaatcctgt gtatttctgc 2521 tgttatgact attttgctgc aaatgatccc acgtcaatcc atgttgttgt ctttagtcta 2581 gaagagccct atgagatcca gctgaaccaa gtgattttct ggctcagttt cctgaagtcc 2641 cttgtcccag ttgaagaacc catagccttc ggtggcaagc tgaagaaccc actccaagtt 2701 gtcctggtgg ccacccacgc tgacatcatg aatgttcctc gaccggctgg aggcgagttt 2761 ggatatgaca aagacacatc gttgctgaaa gagattagga acaggtttgg aaatgatctt 2821 cacatttcaa ataagctgtt tgttctggat gctggggctt ctgggtcaaa ggacatgaag 2881 gtacttcgaa atcatctgca agaaatacga agccagattg tttcggtctg tcctcccatg 2941 actcacctgt gtgagaaaat catctccacg ctgccttcct ggaggaagct caatggaccc 3001 aaccagctga tgtcgctgca gcagtttgtg tacgacgtgc aggaccagct gaaccccctg 3061 gccagcgagg aggacctcag gcgcattgct cagcagctcc acagcacagg cgagatcaac 3121 atcatgcaaa gtgaaacagt tcaggacgtg ctgctcctgg acccccgctg gctctgcaca 3181 aacgtcctgg ggaagttgct gtccgtggag accccacggg cgctgcacca ctaccggggc 3241 cgctacaccg tggaggacat ccagcgcctg gtgcccgaca gcgacgtgga ggagctgctg 3301 cagatcctcg atgccatgga catctgcgcc cgggacctga gcagcgggac catggtggac 3361 gtcccagccc tgatcaagac agacaacctg caccgctcct gggctgatga ggaggacgag 3421 gtgatggtgt atggtggcgt gcgcatcgtg cccgtggaac acctcacccc cttcccatgt 3481 ggcatctttc acaaggtcca ggtgaacctg tgccggtgga tccaccagca aagccacaga 3541 ggcggacgcg gacatcccgc ttgttggtga atggttgcaa gctgcccaac gtgggggccg 3601 agctgctggt gctgctggtc aaacacgccc aaggcattga ggtccaggtc cgcggcctgg 3661 agacggagaa gatcaagtgc tgcctgctgc tggactcggt gtgcagcacc attgagaacg 3721 tcatggccac cacgctgcca gggctcctga ccgtgaagca ttacctgagc ccccagcagc 3781 tgcgggagca ccatgagccc gtcatgatct accagccacg ggacttcttc cgggcacaga 3841 ctctgaagga aacctcactg accaacacca tgggggggta caaggaaagc ttcagcagca 3901 tcatgtgctt cgggtgtcac gacgtctact cacaggccag cctcggcatg gacatccatg 3961 catcagacct gaacctcctc actcggagga aactgagtcg ccTGCTGGAC CCGCCCGACC 4021 CCCTGGGGAA GGACTGGTGC CTTCTCGCCA TGAACTTAGG CCTCCCTGAC CTCGTGGCAA 4081 AGTACAACAC CAGTAACGGG GCTCCCAAGG ATTTCCTCCC CAGCCCCCTC CACGCCCTGC 4141 TGCGGGAATG GACCACCTAC CCTGAGAGCA CAGTGGGCAC CCTCATGTCC AAACTGAGGG 4201 AGCTGGGTCG CCGGGATGCC GCAGACTTTT TGCTGAAGGC ATCCTCTGTG TTCAAAATCA 4261 ACCTGGATGG CAATGGCCAG GAGGCCTATG CCTCGAGCTG CAACAGCGGC ACCTCTTACA 4321 ATTCCATTAG CTCTGTTGTA TCCCGGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 4381 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 4441 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACCCGTCA 4501 CCAACAGAGA TTCCCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 4561 aagatt