Construct: ORF TRCN0000473899
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002080.1_s317c1
- Derived from:
- ccsbBroadEn_09808
- DNA Barcode:
- GTAAGTGAACCTTCGCATAACGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALG1L (200810)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473899
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | NM_001015050.2 | 99.8% | 99.4% | 403A>G |
| 2 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | XM_017005846.2 | 99.2% | 98.9% | 158_160delCAG;406A>G |
| 3 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | XM_017005847.1 | 99.2% | 98.9% | 158_160delCAG;406A>G |
| 4 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | NM_001195223.1 | 90.1% | 89.8% | 1_60del;463A>G |
| 5 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | XM_017005844.2 | 89.7% | 89.4% | 1_60del;218_220delCAG;466A>G |
| 6 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | XM_017005845.1 | 89.7% | 89.4% | 1_60del;218_220delCAG;466A>G |
| 7 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | XM_024453384.1 | 83.6% | 82.3% | (many diffs) |
| 8 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | XM_024453383.1 | 83.1% | 81.9% | (many diffs) |
| 9 | human | 644974 | ALG1L2 | ALG1 chitobiosyldiphosphodo... | NM_001136152.1 | 41.1% | 37% | (many diffs) |
| 10 | human | 56052 | ALG1 | ALG1 chitobiosyldiphosphodo... | NM_001330504.1 | 40% | 38.3% | (many diffs) |
| 11 | human | 56052 | ALG1 | ALG1 chitobiosyldiphosphodo... | XM_017023458.1 | 40% | 38.3% | (many diffs) |
| 12 | human | 650368 | TSSC2 | tumor suppressing subtransf... | NR_024248.1 | 36.4% | (many diffs) | |
| 13 | human | 56052 | ALG1 | ALG1 chitobiosyldiphosphodo... | XM_017023457.2 | 31.8% | 30.4% | (many diffs) |
| 14 | human | 56052 | ALG1 | ALG1 chitobiosyldiphosphodo... | NM_019109.5 | 31% | 29.6% | (many diffs) |
| 15 | human | 56052 | ALG1 | ALG1 chitobiosyldiphosphodo... | XR_932882.3 | 27.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 627
- ORF length:
- 561
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcggtcggcc ttcatggagc tggatgctgg gagcaggctg gtgatgcatc 121 tccgcgagtg gccagccctg ctggtcagca gcacgggctg gacagagttt gaacaactta 181 ctcttgatgg acacaacctt ccttctcttG TCTGTGTGAT AACAGGGTCG GTGGACCTGG 241 GTGTCTGTCT GCACATGTCC TCCAGTGGCC TGGACCTGCC CATGAAGGTG GTGGACATGT 301 TCGGGTGCTG TTTGCCTGTG TGTGCCGTGA ACTTCAAGTG TTTACATGAG CTGGTGAAAC 361 ATGAAGAAAA TGGCCTGGTC TTTGAGGACT CAGAGGAACT GGCAGCTCTG CAGATGCTTT 421 TCTCAAACTT TCCTGATCCT GCAGGCAAGC TAAACCAGTT TTGGAAGGAC CTGCGGGAGT 481 CGCAGCAGCT CCGATGGGAT GAGAGCTGGG TGCAGACTGT GCTCCCTTTG GTTATGGACA 541 TACAACTCCT GGGCCAGAGG CTAAAACCCC GGGACCCCTG CTGTCCTTCC CGCAGCTTCT 601 TCTCGGAGTC TCAGGGCAAA CCCTTTTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 661 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 721 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTAAGTGA 781 ACCTTCGCAT AACGTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 841 aagatt