Construct: ORF TRCN0000473919
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006693.1_s317c1
- Derived from:
- ccsbBroadEn_06961
- DNA Barcode:
- GCATGACATGTAAACATCTAAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC6A1 (6529)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473919
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6529 | SLC6A1 | solute carrier family 6 mem... | NM_001348250.1 | 99.8% | 99.8% | 1047C>G;1562C>A |
| 2 | human | 6529 | SLC6A1 | solute carrier family 6 mem... | NM_003042.4 | 99.8% | 99.8% | 1047C>G;1562C>A |
| 3 | human | 6529 | SLC6A1 | solute carrier family 6 mem... | XM_005265410.5 | 99.8% | 99.8% | 1047C>G;1562C>A |
| 4 | human | 6529 | SLC6A1 | solute carrier family 6 mem... | XM_005265411.5 | 99.8% | 99.8% | 1047C>G;1562C>A |
| 5 | human | 6529 | SLC6A1 | solute carrier family 6 mem... | XM_011534025.3 | 99.8% | 99.8% | 1047C>G;1562C>A |
| 6 | human | 6529 | SLC6A1 | solute carrier family 6 mem... | XM_011534027.3 | 99.8% | 99.8% | 1047C>G;1562C>A |
| 7 | human | 6529 | SLC6A1 | solute carrier family 6 mem... | XM_017007071.2 | 99.8% | 99.8% | 1047C>G;1562C>A |
| 8 | human | 6529 | SLC6A1 | solute carrier family 6 mem... | XM_017007072.2 | 99.8% | 99.8% | 1047C>G;1562C>A |
| 9 | human | 6529 | SLC6A1 | solute carrier family 6 mem... | NM_001348251.1 | 79.8% | 79.7% | 0_1ins360;687C>G;1202C>A |
| 10 | human | 6529 | SLC6A1 | solute carrier family 6 mem... | NM_001348252.1 | 70.1% | 70.1% | 0_1ins534;513C>G;1028C>A |
| 11 | human | 6529 | SLC6A1 | solute carrier family 6 mem... | NM_001348253.1 | 70.1% | 70.1% | 0_1ins534;513C>G;1028C>A |
| 12 | mouse | 232333 | Slc6a1 | solute carrier family 6 (ne... | NM_178703.4 | 90% | 97.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1863
- ORF length:
- 1797
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaccaacggc agcaaggtgg ccgacgggca gatctccacc gaggtcagcg 121 aggcccctgt ggccaatgac aagcccaaaa ccttggtggt caaggtgcag aagaaggcgg 181 cagacctccc cgaccgggac acgtggaagg gccgcttcga cttcctcatg tcctgtgtgg 241 gctatgccat cggcctgggc aacgtctgga ggttccccta tctctgcggg aaaaatggtg 301 ggggagcctt cctgatcccc tatttcctga cactcatctt tgcgggggtc ccactcttcc 361 tgctggagtg ctccctgggc cagtacacct ccatcggggg gctaggggta tggaagctgg 421 ctcctatgtt caagggcgtg ggccttgcgg ctgctgtgct atcattctgg ctgaacatct 481 actacatcgt catcatctcc tgggccattt actacctgta caactccttc accacgacac 541 tgccgtggaa acagtgcgac aacccctgga acacagaccg ctgcttctcc aactacagca 601 tggtcaacac taccaacatg accagcgctg tggtggagtt ctgggagcgc aacatgcatc 661 agatgacgga cgggctggat aagccaggtc agatccgctg gccactggcc atcacgctgg 721 ccatcgcctg gatccttgtg tatttctgta tctggaaggg tgttggctgg actggaaagg 781 tggtctactt ttcagccaca tacccctaca tcatgctgat catcctgttc ttccgtggag 841 tgacgctgcc cggggccaag gagggcatcc tcttctacat cacacccaac ttccgcaagc 901 tgtctgactc cgaggtgtgg ctggatgcgg caacccagat cttcttctca tacgggctgg 961 gcctggggtc cctgatcgct ctcgggagct acaactcttt ccacaacaat gtctacaggg 1021 actccatcat cgtctgctgc atcaattcgt gcaccagcat gttcgcagga ttcgtcatct 1081 tctccatcgt gggcttcatg gcccatgtca cgaagaggtc cattgctgat gtggcggcct 1141 caggccccgg gctggcgttc ctggcatacc cagaggcggt gacccagctg cctatctccc 1201 cactctgggc catcctcttc ttctccatgc tgttgatgct gggcattgac agccagttct 1261 gcactgtgga gggcttcatc acagccctgg tggatgagta ccccaggctc ctccgcaacc 1321 gcagagagct cttcattgct gctgtctgca tcatctccta cctgatcggt ctctctaaca 1381 tcactcaggg gggtatttat gtcttcaaac tctttgacta ctactctgcc agtggcatga 1441 gccTGCTGTT CCTCGTGTTC TTTGAATGTG TCTCTATTTC CTGGTTTTAC GGTGTCAACC 1501 GATTCTATGA CAATATCCAA GAGATGGTTG GATCCAGGCC CTGCATCTGG TGGAAACTCT 1561 GCTGGTCTTT CTTCACACCA ATCATTGTGG CGGGCGTGTT CATTTTCAGT GCTGTGCAGA 1621 TGACGCAACT CACCATGGGA AACTATGTTT TCCCCAAGTG GGGCCAGGGT GTGGGCTGGC 1681 TGATGGCTCT GTCTTCCATG GTCCTCATCC CCGGGTACAT GGCCTACATG TTCCTCACCT 1741 TAAAGGGCTC CCTGAAGCAG CGCATCCAAG TCATGGTCCA GCCCAGCGAA GACATCGTTC 1801 GCCCAGAGAA TGGTCCTGAG CAGCCCCAGG CGGGCAGCTC CACCAGCAAG GAGGCCTACA 1861 TCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1921 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1981 GGCTTTATAT ATCTTGTGGA AAGGACGAGC ATGACATGTA AACATCTAAA TGACGCGTTA 2041 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt