Construct: ORF TRCN0000473930
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007089.1_s317c1
- Derived from:
- ccsbBroadEn_11162
- DNA Barcode:
- GGACCACCCACTCAATCTATTGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAT5B (6777)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473930
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6777 | STAT5B | signal transducer and activ... | XM_005257626.4 | 78.4% | 78.2% | 1170_1491delinsG |
| 2 | human | 6777 | STAT5B | signal transducer and activ... | NM_012448.4 | 49.5% | 49.4% | 1170_2361delinsG |
| 3 | human | 6777 | STAT5B | signal transducer and activ... | XM_024450897.1 | 49.5% | 49.4% | 1170_2361delinsG |
| 4 | human | 6777 | STAT5B | signal transducer and activ... | XM_024450898.1 | 49.5% | 49.4% | 1170_2361delinsG |
| 5 | human | 6776 | STAT5A | signal transducer and activ... | XM_005257624.3 | 47.7% | 48.6% | (many diffs) |
| 6 | human | 6776 | STAT5A | signal transducer and activ... | NM_001288720.1 | 47.6% | 48.4% | (many diffs) |
| 7 | human | 6776 | STAT5A | signal transducer and activ... | NM_001288718.1 | 45.7% | 46.5% | (many diffs) |
| 8 | human | 6776 | STAT5A | signal transducer and activ... | NM_003152.3 | 45.7% | 46.5% | (many diffs) |
| 9 | human | 6776 | STAT5A | signal transducer and activ... | NM_001288719.1 | 42.3% | 43.1% | (many diffs) |
| 10 | human | 6777 | STAT5B | signal transducer and activ... | XM_017024977.1 | 37.5% | 37.3% | 0_1ins283;2delT;888_2079delinsG |
| 11 | mouse | 20851 | Stat5b | signal transducer and activ... | NM_001113563.1 | 45.8% | 48% | (many diffs) |
| 12 | mouse | 20851 | Stat5b | signal transducer and activ... | NM_011489.3 | 45.8% | 48% | (many diffs) |
| 13 | mouse | 20851 | Stat5b | signal transducer and activ... | XM_017314403.1 | 45.8% | 48% | (many diffs) |
| 14 | mouse | 20851 | Stat5b | signal transducer and activ... | XM_006532723.2 | 44.2% | 40.7% | (many diffs) |
| 15 | mouse | 20850 | Stat5a | signal transducer and activ... | NM_011488.3 | 44.1% | 46.1% | (many diffs) |
| 16 | mouse | 20850 | Stat5a | signal transducer and activ... | XM_006532721.1 | 44.1% | 46.1% | (many diffs) |
| 17 | mouse | 20850 | Stat5a | signal transducer and activ... | XM_006532722.1 | 44.1% | 46.1% | (many diffs) |
| 18 | mouse | 20850 | Stat5a | signal transducer and activ... | XM_017314402.1 | 44.1% | 46.1% | (many diffs) |
| 19 | mouse | 20850 | Stat5a | signal transducer and activ... | NM_001164062.1 | 43.9% | 45.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1236
- ORF length:
- 1170
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgtgtggata caagctcagc agctccaagg agaagccctt catcagatgc 121 aagcgttata tggccagcat tttcccattg aggtgcggca ttatttatcc cagtggattg 181 aaagccaagc atgggactca gtagatcttg ataatccaca ggagaacatt aaggccaccc 241 agctcctgga gggcctggtg caggagctgc agaagaaggc agagcaccag gtgggggaag 301 atgggttttt actgaagatc aagctggggc actatgccac acagctccag aacacgtatg 361 accgctgccc catggagctg gtccgctgca tccgccatat attgtacaat gaacagaggt 421 tggtccgaga agccaacaat ggtagctctc cagctggaag ccttgctgat gccatgtccc 481 agaaacacct ccagatcaac cagacgtttg aggagctgcg actggtcacg caggacacag 541 agaatgagtt aaaaaagctg cagcagactc aggagtactt catcatccag taccaggaga 601 gcctgaggat ccaagctcag tttggcccgc tggcccagct gagcccccag gagcgtctga 661 gccgggagac ggccctccag cagaagcagg tgtctctgga ggcctGGTTG CAGCGTGAGG 721 CACAGACACT GCAGCAGTAC CGCGTGGAGC TGGCCGAGAA GCACCAGAAG ACCCTGCAGC 781 TGCTGCGGAA GCAGCAGACC ATCATCCTGG ATGACGAGCT GATCCAGTGG AAGCGGCGGC 841 AGCAGCTGGC CGGGAACGGC GGGCCCCCCG AGGGCAGCCT GGACGTGCTA CAGTCCTGGT 901 GTGAGAAGTT GGCCGAGATC ATCTGGCAGA ACCGGCAGCA GATCCGCAGG GCTGAGCACC 961 TCTGCCAGCA GCTGCCCATC CCCGGCCCAG TGGAGGAGAT GCTGGCCGAG GTCAACGCCA 1021 CCATCACGGA CATTATCTCA GCCCTGGTGA CCAGCACGTT CATCATTGAG AAGCAGCCTC 1081 CTCAGGTCCT GAAGACCCAG ACCAAGTTTG CAGCCACTGT GCGCCTGCTG GTGGGCGGGA 1141 AGCTGAACGT GCACATGAAC CCCCCCCAGG TGAAGGCCAC CATCATCAGT GAGCAGCAGG 1201 CCAAGTCTCT GCTCAAGAAC GAGAACACCC GCAAGTACCC AACTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTTGGCTTT ATATATCTTG TGGAAAGGAC 1381 GAGGACCACC CACTCAATCT ATTGCGACGC GTTAAGTCga caatcaacct ctggattaca 1441 aaatttgtga aagatt