Construct: ORF TRCN0000473930

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007089.1_s317c1
Derived from:
ccsbBroadEn_11162
DNA Barcode:
GGACCACCCACTCAATCTATTGCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STAT5B (6777)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473930
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6777 STAT5B signal transducer and activ... XM_005257626.4 78.4% 78.2% 1170_1491delinsG
2 human 6777 STAT5B signal transducer and activ... NM_012448.4 49.5% 49.4% 1170_2361delinsG
3 human 6777 STAT5B signal transducer and activ... XM_024450897.1 49.5% 49.4% 1170_2361delinsG
4 human 6777 STAT5B signal transducer and activ... XM_024450898.1 49.5% 49.4% 1170_2361delinsG
5 human 6776 STAT5A signal transducer and activ... XM_005257624.3 47.7% 48.6% (many diffs)
6 human 6776 STAT5A signal transducer and activ... NM_001288720.1 47.6% 48.4% (many diffs)
7 human 6776 STAT5A signal transducer and activ... NM_001288718.1 45.7% 46.5% (many diffs)
8 human 6776 STAT5A signal transducer and activ... NM_003152.3 45.7% 46.5% (many diffs)
9 human 6776 STAT5A signal transducer and activ... NM_001288719.1 42.3% 43.1% (many diffs)
10 human 6777 STAT5B signal transducer and activ... XM_017024977.1 37.5% 37.3% 0_1ins283;2delT;888_2079delinsG
11 mouse 20851 Stat5b signal transducer and activ... NM_001113563.1 45.8% 48% (many diffs)
12 mouse 20851 Stat5b signal transducer and activ... NM_011489.3 45.8% 48% (many diffs)
13 mouse 20851 Stat5b signal transducer and activ... XM_017314403.1 45.8% 48% (many diffs)
14 mouse 20851 Stat5b signal transducer and activ... XM_006532723.2 44.2% 40.7% (many diffs)
15 mouse 20850 Stat5a signal transducer and activ... NM_011488.3 44.1% 46.1% (many diffs)
16 mouse 20850 Stat5a signal transducer and activ... XM_006532721.1 44.1% 46.1% (many diffs)
17 mouse 20850 Stat5a signal transducer and activ... XM_006532722.1 44.1% 46.1% (many diffs)
18 mouse 20850 Stat5a signal transducer and activ... XM_017314402.1 44.1% 46.1% (many diffs)
19 mouse 20850 Stat5a signal transducer and activ... NM_001164062.1 43.9% 45.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1236
ORF length:
1170
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tgtgtggata caagctcagc agctccaagg agaagccctt catcagatgc
121aagcgttata tggccagcat tttcccattg aggtgcggca ttatttatcc cagtggattg
181aaagccaagc atgggactca gtagatcttg ataatccaca ggagaacatt aaggccaccc
241agctcctgga gggcctggtg caggagctgc agaagaaggc agagcaccag gtgggggaag
301atgggttttt actgaagatc aagctggggc actatgccac acagctccag aacacgtatg
361accgctgccc catggagctg gtccgctgca tccgccatat attgtacaat gaacagaggt
421tggtccgaga agccaacaat ggtagctctc cagctggaag ccttgctgat gccatgtccc
481agaaacacct ccagatcaac cagacgtttg aggagctgcg actggtcacg caggacacag
541agaatgagtt aaaaaagctg cagcagactc aggagtactt catcatccag taccaggaga
601gcctgaggat ccaagctcag tttggcccgc tggcccagct gagcccccag gagcgtctga
661gccgggagac ggccctccag cagaagcagg tgtctctgga ggcctGGTTG CAGCGTGAGG
721CACAGACACT GCAGCAGTAC CGCGTGGAGC TGGCCGAGAA GCACCAGAAG ACCCTGCAGC
781TGCTGCGGAA GCAGCAGACC ATCATCCTGG ATGACGAGCT GATCCAGTGG AAGCGGCGGC
841AGCAGCTGGC CGGGAACGGC GGGCCCCCCG AGGGCAGCCT GGACGTGCTA CAGTCCTGGT
901GTGAGAAGTT GGCCGAGATC ATCTGGCAGA ACCGGCAGCA GATCCGCAGG GCTGAGCACC
961TCTGCCAGCA GCTGCCCATC CCCGGCCCAG TGGAGGAGAT GCTGGCCGAG GTCAACGCCA
1021CCATCACGGA CATTATCTCA GCCCTGGTGA CCAGCACGTT CATCATTGAG AAGCAGCCTC
1081CTCAGGTCCT GAAGACCCAG ACCAAGTTTG CAGCCACTGT GCGCCTGCTG GTGGGCGGGA
1141AGCTGAACGT GCACATGAAC CCCCCCCAGG TGAAGGCCAC CATCATCAGT GAGCAGCAGG
1201CCAAGTCTCT GCTCAAGAAC GAGAACACCC GCAAGTACCC AACTTTCTTG TACAAAGTGG
1261TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1321CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTTGGCTTT ATATATCTTG TGGAAAGGAC
1381GAGGACCACC CACTCAATCT ATTGCGACGC GTTAAGTCga caatcaacct ctggattaca
1441aaatttgtga aagatt

Download FASTA (ORF) (Full)