Transcript: Human XM_005257626.4

PREDICTED: Homo sapiens signal transducer and activator of transcription 5B (STAT5B), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
STAT5B (6777)
Length:
2097
CDS:
224..1717

Additional Resources:

NCBI RefSeq record:
XM_005257626.4
NBCI Gene record:
STAT5B (6777)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005257626.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000232137 CATCAGATGCAAGCGTTATAT pLKO_005 269 CDS 100% 15.000 21.000 N STAT5B n/a
2 TRCN0000222159 CGCCATATATTGTACAATGAA pLKO.1 551 CDS 100% 5.625 7.875 N STAT5B n/a
3 TRCN0000232140 TATGTCCCTGAAACGAATTAA pLKO_005 1477 CDS 100% 15.000 10.500 N STAT5B n/a
4 TRCN0000232139 TCCGCTGCATCCGCCATATAT pLKO_005 540 CDS 100% 15.000 10.500 N STAT5B n/a
5 TRCN0000222161 CCAGTTCAGTGTTGGTGGAAA pLKO.1 1561 CDS 100% 4.950 3.465 N STAT5B n/a
6 TRCN0000012556 TCTTGATAATCCACAGGAGAA pLKO.1 364 CDS 100% 4.050 2.835 N Stat5b n/a
7 TRCN0000222162 CCTTCATCAGATGCAAGCGTT pLKO.1 265 CDS 100% 2.640 1.848 N STAT5B n/a
8 TRCN0000232138 CCATTGAGGTGCGGCATTATT pLKO_005 303 CDS 100% 15.000 9.000 N STAT5B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005257626.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11162 pDONR223 100% 78.4% 78.2% None 1170_1491delinsG n/a
2 ccsbBroad304_11162 pLX_304 0% 78.4% 78.2% V5 1170_1491delinsG n/a
3 TRCN0000473930 GGACCACCCACTCAATCTATTGCG pLX_317 45.4% 78.4% 78.2% V5 1170_1491delinsG n/a
4 ccsbBroadEn_07008 pDONR223 100% 56.5% 58.8% None (many diffs) n/a
5 ccsbBroad304_07008 pLX_304 0% 56.5% 58.8% V5 (many diffs) n/a
6 TRCN0000473086 GCAGCTAGCTCAGGACTTTAACCG pLX_317 15% 56.5% 58.8% V5 (many diffs) n/a
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