Construct: ORF TRCN0000473968
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000269.1_s317c1
- Derived from:
- ccsbBroadEn_15682
- DNA Barcode:
- AATATCTGTCGGCCTTAAACCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RABGAP1L (9910)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473968
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9910 | RABGAP1L | RAB GTPase activating prote... | NM_001035230.2 | 99.5% | 98.6% | 6G>A |
| 2 | human | 9910 | RABGAP1L | RAB GTPase activating prote... | NM_001243763.1 | 99.5% | 98.6% | 6G>A |
| 3 | human | 9910 | RABGAP1L | RAB GTPase activating prote... | XM_024451295.1 | 99.5% | 98.6% | 6G>A |
| 4 | human | 9910 | RABGAP1L | RAB GTPase activating prote... | NM_001366454.1 | 27.7% | 27.6% | 6G>A;205_205delAinsTTTGTATATTT;207_726del |
| 5 | human | 9910 | RABGAP1L | RAB GTPase activating prote... | NM_001330989.2 | 21.8% | 21.7% | 6G>A;205_205delAinsTTTGTATATTT;207_924del |
| 6 | human | 9910 | RABGAP1L | RAB GTPase activating prote... | XM_017002996.1 | 21.8% | 21.7% | 6G>A;205_205delAinsTTTGTATATTT;207_924del |
| 7 | human | 9910 | RABGAP1L | RAB GTPase activating prote... | XM_017002997.1 | 21.8% | 21.7% | 6G>A;205_205delAinsTTTGTATATTT;207_924del |
| 8 | human | 9910 | RABGAP1L | RAB GTPase activating prote... | XR_922004.1 | 6.8% | 1_2650del;2656G>A;2867_3154del | |
| 9 | human | 9910 | RABGAP1L | RAB GTPase activating prote... | NR_158982.1 | 2.3% | (many diffs) | |
| 10 | mouse | 29809 | Rabgap1l | RAB GTPase activating prote... | XM_006496883.3 | 93.5% | 94.4% | (many diffs) |
| 11 | mouse | 29809 | Rabgap1l | RAB GTPase activating prote... | XM_006496881.3 | 20.5% | 20.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 272
- ORF end:
- 488
- ORF length:
- 216
- Sequence:
-
1 CACCTCGATT AGTTCTCGAG CTTTTGGAGT ACGTCGTCTT TAGGTTGGGG GGAGGGGTTT 61 TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC 121 TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG 181 CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA 241 TTGATCAACA AGTTTGTACA AAAAAGTTGG CATGATAGAA GAAATCTCCA TTATGGTAGC 301 CTATGACGCC CATGTTTTCA GCCAGCTGCA CGATGAAGAC TTCCTCACTA GTCTGGTGGC 361 CATCAGCAAG CCCAGGTCTA TGGTACCAAC CAAGAAGCTG AAGAAATATG AGAAAGAATA 421 TCAGACAATG CGAGAGAGTC AGCTGCAACA GGAAGACCCA ATGGATAGAT ACAAGTTTGT 481 ATATTTGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC 541 TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT 601 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAATATCT GTCGGCCTTA AACCATGACG 661 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt