Construct: ORF TRCN0000473983

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014783.1_s317c1
Derived from:
ccsbBroadEn_06705
DNA Barcode:
GAACATACGTAAATTATCTCATAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDE9A (5152)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473983
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5152 PDE9A phosphodiesterase 9A NM_001001567.1 99.8% 100% 9C>G;1311T>C;1317A>C
2 human 5152 PDE9A phosphodiesterase 9A NM_001001574.1 94.9% 95.1% (many diffs)
3 human 5152 PDE9A phosphodiesterase 9A NM_001001571.1 92.1% 91.9% (many diffs)
4 human 5152 PDE9A phosphodiesterase 9A NM_001001570.1 92% 93% (many diffs)
5 human 5152 PDE9A phosphodiesterase 9A NM_001001578.1 91.9% 85.9% (many diffs)
6 human 5152 PDE9A phosphodiesterase 9A NM_001001583.1 90.8% 85.4% (many diffs)
7 human 5152 PDE9A phosphodiesterase 9A NM_002606.3 89.7% 89.8% (many diffs)
8 human 5152 PDE9A phosphodiesterase 9A NM_001315533.1 89.2% 84.3% (many diffs)
9 human 5152 PDE9A phosphodiesterase 9A XM_024452084.1 88.3% 77.4% (many diffs)
10 human 5152 PDE9A phosphodiesterase 9A NM_001001581.1 87.3% 77.9% (many diffs)
11 human 5152 PDE9A phosphodiesterase 9A NM_001001568.1 87.1% 86.6% (many diffs)
12 human 5152 PDE9A phosphodiesterase 9A NM_001001569.1 87% 85.2% (many diffs)
13 human 5152 PDE9A phosphodiesterase 9A XM_011529598.2 86.6% 76% (many diffs)
14 human 5152 PDE9A phosphodiesterase 9A NM_001001577.1 85.9% 85.7% (many diffs)
15 human 5152 PDE9A phosphodiesterase 9A NM_001001582.1 82.8% 82.6% (many diffs)
16 human 5152 PDE9A phosphodiesterase 9A NM_001001575.1 80.8% 80.4% (many diffs)
17 human 5152 PDE9A phosphodiesterase 9A NM_001001572.1 72.2% 72.4% 0_1ins441;870T>C;876A>C
18 human 5152 PDE9A phosphodiesterase 9A NM_001001573.1 72.2% 72.4% 0_1ins441;870T>C;876A>C
19 human 5152 PDE9A phosphodiesterase 9A NM_001001579.1 72.2% 72.4% 0_1ins441;870T>C;876A>C
20 human 5152 PDE9A phosphodiesterase 9A NM_001001584.2 72.2% 72.4% 0_1ins441;870T>C;876A>C
21 human 5152 PDE9A phosphodiesterase 9A NM_001001585.1 72.2% 72.4% 0_1ins441;870T>C;876A>C
22 human 5152 PDE9A phosphodiesterase 9A XM_017028366.1 72.2% 72.4% 0_1ins441;870T>C;876A>C
23 human 5152 PDE9A phosphodiesterase 9A XM_011529600.2 71.6% 66.9% (many diffs)
24 human 5152 PDE9A phosphodiesterase 9A NM_001001576.1 70.3% 70.3% (many diffs)
25 human 5152 PDE9A phosphodiesterase 9A NM_001001580.1 70.3% 70.3% (many diffs)
26 human 5152 PDE9A phosphodiesterase 9A XM_017028367.1 41.7% 35.4% (many diffs)
27 mouse 18585 Pde9a phosphodiesterase 9A NM_001163748.1 86.7% 92.1% (many diffs)
28 mouse 18585 Pde9a phosphodiesterase 9A NM_008804.4 86.7% 92.1% (many diffs)
29 mouse 18585 Pde9a phosphodiesterase 9A XR_001782057.1 63.6% (many diffs)
30 mouse 18585 Pde9a phosphodiesterase 9A XR_001782058.1 63.5% (many diffs)
31 mouse 18585 Pde9a phosphodiesterase 9A XM_006523821.2 62.6% 65.6% (many diffs)
32 mouse 18585 Pde9a phosphodiesterase 9A XM_017317319.1 62.6% 65.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1665
ORF length:
1599
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg atcgggctcc tccagctacc ggcccaaggc catctacctg gacatcgatg
121gacgcattca gaaggtaatc ttcagcaagt actgcaactc cagcgacatc atggacctgt
181tctgcatcgc caccggcctg cctcggaaca cgaccatctc cctgctgacc accgacgacg
241ccatggtctc catcgacccc accatgcccg cgaattcaga acgcactccg tacaaagtga
301gacctgtggc catcaagcaa ctctccgaga gagaagaatt aatccagagc gtgctggcgc
361aggttgcaga gcagttctca agagcattca aaatcaatga actgaaagct gaagttgcaa
421atcacttggc tgtcctagag aaacgcgtgg aattggaagg actaaaagtg gtggagattg
481agaaatgcaa gagtgacatt aagaagatga gggaggagct ggcggccaga agcagcagga
541ccaactgccc ctgtaagtac agttttttgg ataaccacaa gaagttgact cctcgacgcg
601atgttcccac ttaccccaag tacctgctct ctccagagac catcgaggcc ctgcggaagc
661cgacctttga cgtctggctt tgggagccca atgagatgct gagctgcctg gagcacatgt
721accacgacct cgggctggtc agggacttca gcatcaaccc tgtcaccctc aggaggtggc
781tgttctgcgt ccacgacaac tacagaaaca accccttcca caacttccgg cactgcttct
841gcgtggccca gatgatgtac agcatggtct ggctctgcag tctccaggag aagttctcac
901aaacggatat cctgatccta atgacagcgg ccatctgcca cgatctggac catcccggct
961acaacaacac gtaccagatc aatgcccgca cagagctggc ggtccgctac aatgacatct
1021caccgctgga gaaccaccac tgcgccgtgg ccttccagat cctcgccgag cctgagtgca
1081acatcttctc caacatccca cctgatgggt tcaagcagat ccgacaggga atgatcacat
1141taatcttggc cactgacatg gCAAGACATG CAGAAATTAT GGATTCTTTC AAAGAGAAAA
1201TGGAGAATTT TGACTACAGC AACGAGGAGC ACATGACCCT GCTGAAGATG ATTTTGATAA
1261AATGCTGTGA TATCTCTAAC GAGGTCCGTC CAATGGAAGT CGCAGAGCCT TGGGTGGACT
1321GTTTATTAGA GGAATATTTT ATGCAGAGCG ACCGTGAGAA GTCAGAAGGC CTTCCCGTGG
1381CCCCGTTCAT GGACCGAGAC AAAGTGACCA AGGCCACAGC CCAGATTGGG TTCATCAAGT
1441TTGTCCTGAT CCCAATGTTT GAAACAGTGA CCAAGCTCTT CCCCATGGTT GAGGAGATCA
1501TGCTGCAGCC ACTTTGGGAA TCCCGAGATC GCTACGAGGA GCTGAAGCGG ATAGATGACG
1561CCATGAAAGA GTTACAGAAG AAGACTGACA GCTTGACGTC TGGGGCCACC GAGAAGTCCA
1621GAGAGAGAAG CAGAGATGTG AAAAACAGTG AAGGAGACTG TGCCAGCCCA ACTTTCTTGT
1681ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1741AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1801GAAAGGACGA GAACATACGT AAATTATCTC ATATACGCGT TAAGTCgaca atcaacctct
1861ggattacaaa atttgtgaaa gatt

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