Construct: ORF TRCN0000473995
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013829.1_s317c1
- Derived from:
- ccsbBroadEn_12547
- DNA Barcode:
- CGCGATTGGAGGAAGGCCAGTAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFAF5 (79133)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473995
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NM_001352406.2 | 100% | 100% | |
| 2 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NM_001352407.2 | 100% | 100% | |
| 3 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NM_001352403.2 | 84% | 84% | 1_90del |
| 4 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | XM_006723624.2 | 84% | 84% | 1_90del |
| 5 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | XM_024451999.1 | 84% | 84% | 1_90del |
| 6 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NM_001039375.3 | 49.8% | 49.8% | 1_477del |
| 7 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NM_024120.5 | 45.7% | 45.7% | 1_561del |
| 8 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | XR_937140.2 | 32.1% | (many diffs) | |
| 9 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NM_001352408.1 | 16.6% | 15.2% | (many diffs) |
| 10 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | XR_430269.3 | 9.8% | (many diffs) | |
| 11 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NR_147980.2 | 8.7% | 1_498del;713_810del;1071_5443del | |
| 12 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | XR_001754396.1 | 8.6% | 1_520del;735_832del;1093_5465del | |
| 13 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NR_147978.2 | 8.6% | 1_602del;758_813del;1133_5505del | |
| 14 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NR_029377.2 | 8.5% | 1_602del;817_914del;1175_5547del | |
| 15 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NR_147979.2 | 8.5% | 1_622del;837_934del;1195_5567del | |
| 16 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NR_147983.2 | 8.4% | 1_652del;867_964del;1225_5597del | |
| 17 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NR_147981.2 | 8.3% | 1_736del;951_1048del;1309_5681del | |
| 18 | human | 79133 | NDUFAF5 | NADH:ubiquinone oxidoreduct... | NR_147982.2 | 8.2% | (many diffs) | |
| 19 | mouse | 69487 | Ndufaf5 | NADH:ubiquinone oxidoreduct... | XM_030252053.1 | 87.7% | 90.5% | (many diffs) |
| 20 | mouse | 69487 | Ndufaf5 | NADH:ubiquinone oxidoreduct... | XM_030252052.1 | 73.7% | 76% | (many diffs) |
| 21 | mouse | 69487 | Ndufaf5 | NADH:ubiquinone oxidoreduct... | XM_011239768.2 | 43.7% | 45.1% | (many diffs) |
| 22 | mouse | 69487 | Ndufaf5 | NADH:ubiquinone oxidoreduct... | NM_027093.4 | 40.1% | 41.4% | (many diffs) |
| 23 | mouse | 69487 | Ndufaf5 | NADH:ubiquinone oxidoreduct... | XR_003953716.1 | 2.8% | (many diffs) | |
| 24 | mouse | 69487 | Ndufaf5 | NADH:ubiquinone oxidoreduct... | XR_003953715.1 | 2.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 564
- ORF length:
- 474
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTTTGGAGG CGACACACTC TATGAACTTC 121 GGTGTTCCTT ACAGTTAGCG GAAACGGAAA GGGAAGGAGG ATTTTCTCCA CACATTTCTC 181 CTTTCACTGC TGTCAATGAC CTGGGACATC TGCTTGGGAG AGCTGGCTTT AATACTCTGA 241 CTGTGGACAC TGATGAAATT CAAGTTAACT ATCCTGGAAT GTTTGAATTG ATGGAAGATT 301 TACAAGGTAT GGGTGAGAGT AACTGTGCTT GGAATAGAAA AGCCCTGCTG CATCGAGACA 361 CAATGCTGGC AGCTGCGGCA GTGTACAGAG AAATGTACAG AAATGAAGAT GGTTCAGTAC 421 CTGCTACATA CCAGATCTAT TACATGATAG GATGGAAATA TCATGAGTCA CAGGCAAGAC 481 CAGCTGAAAG AGGTTCCGCA ACTGTGTCAT TTGGAGAGCT AGGAAAAATA AACAACCTTA 541 TGCCACCGGG GAAAAAATCA CAATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 601 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 661 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GCGATTGGAG 721 GAAGGCCAGT AGCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 781 att