Construct: ORF TRCN0000474000
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019053.3_s317c1
- Derived from:
- ccsbBroadEn_14525
- DNA Barcode:
- CGACTCCTGGAGTTTTTCATCTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABL1 (25)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474000
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25 | ABL1 | ABL proto-oncogene 1, non-r... | NM_005157.6 | 99.9% | 100% | 3075C>T;3390delG |
| 2 | human | 25 | ABL1 | ABL proto-oncogene 1, non-r... | NM_007313.2 | 97.6% | 96.5% | (many diffs) |
| 3 | mouse | 11350 | Abl1 | c-abl oncogene 1, non-recep... | NM_009594.4 | 84.2% | 87.7% | (many diffs) |
| 4 | mouse | 11350 | Abl1 | c-abl oncogene 1, non-recep... | NM_001283045.1 | 83% | 84.8% | (many diffs) |
| 5 | mouse | 11350 | Abl1 | c-abl oncogene 1, non-recep... | NM_001283046.1 | 82.9% | 85.3% | (many diffs) |
| 6 | mouse | 11350 | Abl1 | c-abl oncogene 1, non-recep... | NM_001112703.2 | 82.4% | 84.7% | (many diffs) |
| 7 | mouse | 11350 | Abl1 | c-abl oncogene 1, non-recep... | NM_001283047.1 | 65.5% | 67% | (many diffs) |
| 8 | mouse | 11350 | Abl1 | c-abl oncogene 1, non-recep... | XM_006497621.3 | 44.3% | 45.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3459
- ORF length:
- 3390
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttggagatc tgcctgaagc tggtgggctg caaatccaag aaggggctgt 121 cctcgtcctc cagctgttat ctggaagaag cccttcagcg gccagtagca tctgactttg 181 agcctcaggg tctgagtgaa gccgctcgtt ggaactccaa ggaaaacctt ctcgctggac 241 ccagtgaaaa tgaccccaac cttttcgttg cactgtatga ttttgtggcc agtggagata 301 acactctaag cataactaaa ggtgaaaagc tccgggtctt aggctataat cacaatgggg 361 aatggtgtga agcccaaacc aaaaatggcc aaggctgggt cccaagcaac tacatcacgc 421 cagtcaacag tctggagaaa cactcctggt accatgggcc tgtgtcccgc aatgccgctg 481 agtatctgct gagcagcggg atcaatggca gcttcttggt gcgtgagagt gagagcagtc 541 ctggccagag gtccatctcg ctgagatacg aagggagggt gtaccattac aggatcaaca 601 ctgcttctga tggcaagctc tacgtctcct ccgagagccg cttcaacacc ctggccgagt 661 tggttcatca tcattcaacg gtggccgacg ggctcatcac cacgctccat tatccagccc 721 caaagcgcaa caagcccact gtctatggtg tgtcccccaa ctacgacaag tgggagatgg 781 aacgcacgga catcaccatg aagcacaagc tgggcggggg ccagtacggg gaggtgtacg 841 agggcgtgtg gaagaaatac agcctgacgg tggccgtgaa gaccttgaag gaggacacca 901 tggaggtgga agagttcttg aaagaagctg cagtcatgaa agagatcaaa caccctaacc 961 tggtgcagct ccttggggtc tgcacccggg agcccccgtt ctatatcatc actgagttca 1021 tgacctacgg gaacctcctg gactacctga gggagtgcaa ccggcaggag gtgaacgccg 1081 tggtgctgct gtacatggcc actcagatct cgtcagccat ggagtacctg gagaagaaaa 1141 acttcatcca cagagatctt gctgcccgaa actgcctggt aggggagaac cacttggtga 1201 aggtagctga ttttggcctg agcaggttga tgacagggga cacctacaca gcccatgctg 1261 gagccaagtt ccccatcaaa tggactgcac ccgagagcct ggcctacaac aagttctcca 1321 tcaagtccga cgtctgggca tttggagtat tgctttggga aattgctacc tatggcatgt 1381 ccccttaccc gggaattgac ctgtcccagg tgtatgagct gctagagaag gactaccgca 1441 tggagcgccc agaaggctgc ccagagaagg tctatgaact catgcgagca tgttggcagt 1501 ggaatccctc tgaccggccc tcctttgctg aaatccacca agcctttgaa acaatgttcc 1561 aggaatccag tatctcagac gaagtggaaa aggagctggg gaaacaaggc gtccgtgggg 1621 ctgtgagtac cttgctgcag gccccagagc tgcccaccaa gacgaggacc tccaggagag 1681 ctgcagagca cagagacacc actgacgtgc ctgagatgcc tcactccaag ggccagggag 1741 agagcgatcc tctggaccat gagcctgccg tgtctccatt gctccctcga aaagagcgag 1801 gtcccccgga gggcggcctg aatgaagatg agcgccttct ccccaaagac aaaaagacca 1861 acttgttcag cgccttgatc aagaagaaga agaagacagc cccaacccct cccaaacgca 1921 gcagctcctt ccgggagatg gacggccagc cggagcgcag aggggccggc gaggaagagg 1981 gccgagacat cagcaacggg gcactggctt tcaccccctt ggacacagct gacccagcca 2041 agtccccaaa gcccagcaat ggggctgggg tccccaatgg agccctccgg gagtccgggg 2101 gctcaggctt ccggtctccc cacctgtgga agaagtccag cacgctgacc agcagccgcc 2161 tagccaccgg cgaggaggag ggcggtggca gctccagcaa gcgcttcctg cgctcttgct 2221 ccgcctcctg cgttccccat ggggccaagg acacggagtg gaggtcagtc acgctgcctc 2281 gggacttgca gtccacggga agacagtttg actcgtccac atttggaggg cacaaaagtg 2341 agaagccggc tctgcctcgg aagagggcag gggagaacag gtctgaccag gtgacccgag 2401 gcacagtaac gcctcccccc aggctggtga aaaagaatga ggaagctgct gatgaggtct 2461 tcaaagacat catggagtcc agcccgggct ccagcccgcc caacctgact ccaaaacccc 2521 tccggcggca ggtcaccgtg gcccctgcct cgggcctccc ccacaaggaa gaagctggaa 2581 agggcagtgc cttagggacc cctgctgcag ctgagccagt gacccccacc agcaaagcag 2641 gctcaggtgc accagggggc accagcaagg gccccgccga ggagtccaga gtgaggaggc 2701 acaagcactc ctctgagtcg ccagggaggg acaaggggaa attgtccagg ctcaaacctg 2761 ccccgccgcc cccaccagca gcctctgcag ggaaggctgg aggaaagccc tcgcagagcc 2821 cgagccagga ggcggccggg gaggcagtcc tgggcgcaaa gacaaaagcc acgagtctgg 2881 ttgatgctgt gaacagtgac gctgccaagc ccagccagcc gggagagggc ctcaaaaagc 2941 ccgtgctccc ggccactcca aagccacagt ccgccaagcc gtcggggacc cccatcagcc 3001 cagcccccgt tcccTCCACG TTGCCATCAG CATCCTCGGC CCTGGCAGGG GACCAGCCGT 3061 CTTCCACCGC CTTCATCCCT CTCATATCAA CCCGAGTGTC TCTTCGGAAA ACCCGCCAGC 3121 CTCCAGAGCG GATCGCCAGC GGTGCCATCA CCAAGGGCGT GGTCCTGGAC AGCACCGAGG 3181 CGCTGTGCCT CGCCATCTCT AGGAACTCCG AGCAGATGGC CAGCCACAGC GCAGTGCTGG 3241 AGGCCGGCAA AAACCTCTAC ACGTTCTGCG TGAGCTATGT GGATTCCATC CAGCAAATGA 3301 GGAACAAGTT TGCCTTCCGA GAGGCCATCA ACAAACTGGA GAATAATCTC CGGGAGCTTC 3361 AGATCTGCCC GGCGACAGCA GGCAGTGGTC CAGCGGCCAC TCAGGACTTC AGCAAGCTCC 3421 TCAGTTCGGT GAAGGAAATC AGTGACATAG TGCAGAGGCC AACTTTCTTG TACAAAGTGG 3481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 3541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3601 ACGACTCCTG GAGTTTTTCA TCTATACGCG TTAAGTCgac aatcaacctc tggattacaa 3661 aatttgtgaa agatt