Construct: ORF TRCN0000474079
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017643.1_s317c1
- Derived from:
- ccsbBroadEn_01925
- DNA Barcode:
- CACCGTCAAGCTCCTCGGATAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STX7 (8417)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474079
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8417 | STX7 | syntaxin 7 | NM_001326578.2 | 100% | 100% | |
| 2 | human | 8417 | STX7 | syntaxin 7 | NM_001326579.1 | 100% | 100% | |
| 3 | human | 8417 | STX7 | syntaxin 7 | NM_003569.3 | 100% | 100% | |
| 4 | human | 8417 | STX7 | syntaxin 7 | NM_001326580.2 | 89.8% | 89.2% | (many diffs) |
| 5 | human | 8417 | STX7 | syntaxin 7 | NR_137169.2 | 4.4% | 1_168del;705_706ins73;879_15772del | |
| 6 | mouse | 53331 | Stx7 | syntaxin 7 | NM_016797.4 | 87% | 94.2% | (many diffs) |
| 7 | mouse | 53331 | Stx7 | syntaxin 7 | XM_006512800.2 | 87% | 94.2% | (many diffs) |
| 8 | mouse | 53331 | Stx7 | syntaxin 7 | XM_006512801.3 | 87% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 849
- ORF length:
- 783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaagg 61 ttggcatgtc ttacactcca ggagttggtg gtgaccccgc ccagttggcc cagaggatct 121 cttctaacat ccagaagatc acacagtgtt ctgtggaaat acaaagaact ctgaatcaac 181 ttggaacacc tcaagattca cctgaattga ggcaacagtt gcaacagaag cagcagtata 241 ctaaccagct tgccaaagaa acagataagt acattaaaga gtttggATCT CTGCCCACCA 301 CCCCCAGTGA ACAGCGTCAA AGGAAAATAC AGAAGGATCG CTTAGTGGCA GAGTTCACAA 361 CATCACTGAC AAACTTCCAG AAGGTCCAGA GGCAGGCTGC TGAGCGAGAG AAAGAGTTTG 421 TTGCTCGAGT AAGAGCCAGT TCCAGAGTGT CTGGCAGTTT TCCTGAGGAC AGCTCAAAAG 481 AAAGGAATCT TGTATCCTGG GAAAGCCAAA CTCAACCTCA AGTGCAGGTG CAGGATGAAG 541 AAATTACAGA GGATGACCTC CGTCTTATTC ATGAGAGAGA ATCTTCTATC AGGCAACTTG 601 AAGCTGATAT TATGGATATT AATGAAATAT TTAAAGATTT GGGAATGATG ATTCATGAAC 661 AAGGAGATGT AATAGATAGC ATAGAAGCCA ATGTGGAAAA TGCAGAGGTG CACGTTCAGC 721 AAGCAAATCA GCAGCTGTCA AGGGCAGCAG ATTATCAGCG CAAATCCAGA AAAACCCTGT 781 GCATCATCAT TCTTATCCTT GTCATTGGAG TTGCGATTAT CAGTCTCATC ATATGGGGAT 841 TGAACCACTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 901 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 961 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACACCGT CAAGCTCCTC GGATAATCAC 1021 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt