Construct: ORF TRCN0000474090
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008216.1_s317c1
- Derived from:
- ccsbBroadEn_00215
- DNA Barcode:
- TACAAGAACCAATTATGGAGAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAPN3 (825)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474090
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 825 | CAPN3 | calpain 3 | NM_173088.1 | 100% | 100% | |
| 2 | human | 825 | CAPN3 | calpain 3 | NM_173089.1 | 50.4% | 50.4% | 0_1ins459 |
| 3 | human | 825 | CAPN3 | calpain 3 | NM_173090.1 | 50.4% | 50.4% | 0_1ins459 |
| 4 | human | 825 | CAPN3 | calpain 3 | NM_000070.3 | 37.6% | 37.6% | 1_1536del |
| 5 | human | 825 | CAPN3 | calpain 3 | NM_024344.1 | 36.9% | 36.9% | 1_1536del;1781_1782insGAAAAAGAAAAAAACCAA |
| 6 | human | 825 | CAPN3 | calpain 3 | NM_173087.1 | 34.2% | 34.2% | 1_1392del;1637_1638ins132 |
| 7 | mouse | 12335 | Capn3 | calpain 3 | NM_007601.3 | 33.9% | 34.9% | (many diffs) |
| 8 | mouse | 12335 | Capn3 | calpain 3 | NM_001109761.2 | 31.9% | 33.5% | (many diffs) |
| 9 | mouse | 12335 | Capn3 | calpain 3 | NM_001177799.1 | 31.1% | 32.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 993
- ORF length:
- 927
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca cgggaacaag cagcacctgc agaaggactt cttcctgtac aacgcctcca 121 aggccaggag caaaacctac atcaacatgc gggaggtgtc ccagcgcttc cgcctgcctc 181 ccagcgagta cgtcatcgtg ccctccacct acgagcccca ccaggagggg gaattcatcc 241 tccgggtctt ctctgaaaag aggaacctct ctgaggaagt tgaaaatacc atctccgtgg 301 atcggccagt gaaaaagaaa aaaaccaagc ccatcatctt cgtttcggac agagcaaaca 361 gcaacaagga gctgggtgtg gaccaggagt cagaggaggg caaaggcaaa acaagccctg 421 ataagcaaaa gcagtcccca cagccacagc ctggcagctc TGATCAGGAA AGTGAGGAAC 481 AGCAACAATT CCGGAACATT TTCAAGCAGA TAGCAGGAGA TGACATGGAG ATCTGTGCAG 541 ATGAGCTCAA GAAGGTCCTT AACACAGTCG TGAACAAACA CAAGGACCTG AAGACACACG 601 GGTTCACACT GGAGTCCTGC CGTAGCATGA TTGCGCTCAT GGATACAGAT GGCTCTGGAA 661 AGCTCAACCT GCAGGAGTTC CACCACCTCT GGAACAAGAT TAAGGCCTGG CAGAAAATTT 721 TCAAACACTA TGACACAGAC CAGTCCGGCA CCATCAACAG CTACGAGATG CGAAATGCAG 781 TCAACGACGC AGGATTCCAC CTCAACAACC AGCTCTATGA CATCATTACC ATGCGGTACG 841 CAGACAAACA CATGAACATC GACTTTGACA GTTTCATCTG CTGCTTCGTT AGGCTGGAGG 901 GCATGTTCAG AGCTTTTCAT GCATTTGACA AGGATGGAGA TGGTATCATC AAGCTCAACG 961 TTCTGGAGTG GCTGCAGCTC ACCATGTATG CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1021 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1081 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1141 CAAGAACCAA TTATGGAGAG ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 1201 ttgtgaaaga tt