Construct: ORF ccsbBroadEn_00215

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008216.1_s300c1, BRDN0000385099
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CAPN3 (825)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 825 CAPN3 calpain 3 NM_173088.1 100% 100%
2 human 825 CAPN3 calpain 3 NM_173089.1 50.4% 50.4% 0_1ins459
3 human 825 CAPN3 calpain 3 NM_173090.1 50.4% 50.4% 0_1ins459
4 human 825 CAPN3 calpain 3 NM_000070.3 37.6% 37.6% 1_1536del
5 human 825 CAPN3 calpain 3 NM_024344.1 36.9% 36.9% 1_1536del;1781_1782insGAAAAAGAAAAAAACCAA
6 human 825 CAPN3 calpain 3 NM_173087.1 34.2% 34.2% 1_1392del;1637_1638ins132
7 mouse 12335 Capn3 calpain 3 NM_007601.3 33.9% 34.9% (many diffs)
8 mouse 12335 Capn3 calpain 3 NM_001109761.2 31.9% 33.5% (many diffs)
9 mouse 12335 Capn3 calpain 3 NM_001177799.1 31.1% 32.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
993
ORF length:
927
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCA CGGGAACAAG CAGCACCTGC AGAAGGACTT CTTCCTGTAC AACGCCTCCA
121AGGCCAGGAG CAAAACCTAC ATCAACATGC GGGAGGTGTC CCAGCGCTTC CGCCTGCCTC
181CCAGCGAGTA CGTCATCGTG CCCTCCACCT ACGAGCCCCA CCAGGAGGGG GAATTCATCC
241TCCGGGTCTT CTCTGAAAAG AGGAACCTCT CTGAGGAAGT TGAAAATACC ATCTCCGTGG
301ATCGGCCAGT GAAAAAGAAA AAAACCAAGC CCATCATCTT CGTTTCGGAC AGAGCAAACA
361GCAACAAGGA GCTGGGTGTG GACCAGGAGT CAGAGGAGGG CAAAGGCAAA ACAAGCCCTG
421ATAAGCAAAA GCAGTCCCCA CAGCCACAGC CTGGCAGCTC TGATCAGGAA AGTGAGGAAC
481AGCAACAATT CCGGAACATT TTCAAGCAGA TAGCAGGAGA TGACATGGAG ATCTGTGCAG
541ATGAGCTCAA GAAGGTCCTT AACACAGTCG TGAACAAACA CAAGGACCTG AAGACACACG
601GGTTCACACT GGAGTCCTGC CGTAGCATGA TTGCGCTCAT GGATACAGAT GGCTCTGGAA
661AGCTCAACCT GCAGGAGTTC CACCACCTCT GGAACAAGAT TAAGGCCTGG CAGAAAATTT
721TCAAACACTA TGACACAGAC CAGTCCGGCA CCATCAACAG CTACGAGATG CGAAATGCAG
781TCAACGACGC AGGATTCCAC CTCAACAACC AGCTCTATGA CATCATTACC ATGCGGTACG
841CAGACAAACA CATGAACATC GACTTTGACA GTTTCATCTG CTGCTTCGTT AGGCTGGAGG
901GCATGTTCAG AGCTTTTCAT GCATTTGACA AGGATGGAGA TGGTATCATC AAGCTCAACG
961TTCTGGAGTG GCTGCAGCTC ACCATGTATG CCTGCCCAAC TTTCTTGTAC AAAGTtggca
1021ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)