Construct: ORF TRCN0000474116
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009719.1_s317c1
- Derived from:
- ccsbBroadEn_03223
- DNA Barcode:
- CGATTCGGATACCGTCAAGCACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASB3 (51130)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474116
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51130 | ASB3 | ankyrin repeat and SOCS box... | NM_016115.5 | 100% | 100% | |
| 2 | human | 100302652 | GPR75-ASB3 | GPR75-ASB3 readthrough | NM_001164165.1 | 93.1% | 93.1% | 1_114del |
| 3 | human | 51130 | ASB3 | ankyrin repeat and SOCS box... | NM_001201965.2 | 85.9% | 85.9% | 0_1ins219 |
| 4 | human | 51130 | ASB3 | ankyrin repeat and SOCS box... | NM_145863.3 | 85.9% | 85.9% | 0_1ins219 |
| 5 | mouse | 65257 | Asb3 | ankyrin repeat and SOCS box... | NM_023906.3 | 85.1% | 82.6% | (many diffs) |
| 6 | mouse | 65257 | Asb3 | ankyrin repeat and SOCS box... | XM_006514771.3 | 85.1% | 82.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1620
- ORF length:
- 1554
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ttttacagag gcttacgcgg acacgtgctc tacagttgga cttgctgcca 121 gggaaggcaa tgttaaagtc ttaaggaaac tgctcaaaaa gggccgaagt gtcgatgttg 181 ctgataacag gggatggatg ccaattcatg aagcagctta tcacaactct gtagaatgtt 241 tgcaaatgtt aattaatgca gattcatctg aaaactacat taagatgaag acctttgaag 301 gtttctgtgc tttgcatctc gctgcaagtc aaggacattg gaaaatcgta cagattcttt 361 tagaagctgg ggcagatcct aatgcaacta ctttagaaga aacgacacca ttgtttttag 421 ctgttgaaaa tggacagata gatgtgttaa ggctgttgct tcaacacgga gcaaatgtta 481 atggatccca ttctatgtgt ggatggaact ccttgcacca ggcttctttt caggaaaatg 541 ctgagatcat aaaattgctt cttagaaaag gagcaaacaa ggaatgccag gatgactttg 601 gaatcacacc tttatttgtg gctgctcagt atggcaagct agaaagcttg agcatactta 661 tttcatcggg tgcaaatgtc aattgtcaag ccttggacaa agctacaccc ttgttcattg 721 ctgctcaaga gggacacaca aaatgtgtgg agcttttgct ctccagtggg gcagatcctg 781 atctttactg taatgaggac agttggcagt tacctattca tgcagctgca caaatgggcc 841 atacaaaaat cttggacttg ttaataccac ttactaaccg ggcctgtgac actgggctaa 901 acaaagtaag ccctgtttac tcagcagtgt ttgggggaca tgaagattgc ctagaaatat 961 tactccggaa tggctacagc ccagacgccc aggcgtgcct tgtttttgga ttcagttctc 1021 ctgtgtgcat ggctttccaa aaggactgtg agttctttgg aattgtgaac attcttttga 1081 aatatggagc ccagataaat gaacttcatt tggcatactg cctgaagtac gagaagtttt 1141 cgatatttcg ctactttttG AGGAAAGGTT GCTCATTGGG ACCATGGAAC CATATATATG 1201 AATTTGTAAA TCATGCAATT AAAGCACAAG CAAAATATAA GGAGTGGTTG CCACATCTTC 1261 TGGTTGCTGG ATTTGACCCA CTGATTCTAC TGTGCAATTC TTGGATTGAC TCAGTCAGCA 1321 TTGACACCCT TATCTTCACT TTGGAGTTTA CTAATTGGAA GACACTTGCA CCAGCTGTTG 1381 AAAGGATGCT CTCTGCTCGT GCCTCAAACG CTTGGATTCT ACAGCAACAT ATTGCCACTG 1441 TTCCATCCCT GACCCATCTT TGTCGTTTGG AAATTCGGTC CAGTCTAAAA TCAGAACGTC 1501 TACGGTCTGA CAGTTATATT AGTCAGCTGC CACTTCCCAG AAGCCTACAT AATTATTTGC 1561 TCTATGAAGA CGTTCTGAGG ATGTATGAAG TTCCAGAACT GGCAGCTATT CAAGATGGAT 1621 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1681 GTCTCGATTC TACGTAGTAA TGAACTAGCC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1741 TTTATATATC TTGTGGAAAG GACGACGATT CGGATACCGT CAAGCACCTA CGCGTTAAGT 1801 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt