Construct: ORF TRCN0000474153
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015943.1_s317c1
- Derived from:
- ccsbBroadEn_11575
- DNA Barcode:
- TCGCATTATGGCTAGCACCCCGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACOT2 (10965)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474153
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 641371 | ACOT1 | acyl-CoA thioesterase 1 | NM_001037161.2 | 98.9% | 98.8% | (many diffs) |
| 2 | human | 10965 | ACOT2 | acyl-CoA thioesterase 2 | NM_006821.5 | 87% | 86.7% | 1_186del;983A>G;1424A>G |
| 3 | human | 641371 | ACOT1 | acyl-CoA thioesterase 1 | XM_017021590.1 | 68.4% | 65.1% | (many diffs) |
| 4 | human | 10965 | ACOT2 | acyl-CoA thioesterase 2 | NM_001364177.1 | 66.8% | 64.6% | (many diffs) |
| 5 | human | 641371 | ACOT1 | acyl-CoA thioesterase 1 | XM_017021589.1 | 65.9% | 64.3% | (many diffs) |
| 6 | human | 641371 | ACOT1 | acyl-CoA thioesterase 1 | XM_017021591.1 | 61.1% | 61% | (many diffs) |
| 7 | human | 641371 | ACOT1 | acyl-CoA thioesterase 1 | XM_017021592.1 | 61.1% | 61% | (many diffs) |
| 8 | human | 10965 | ACOT2 | acyl-CoA thioesterase 2 | NR_046028.1 | 60.4% | (many diffs) | |
| 9 | human | 10965 | ACOT2 | acyl-CoA thioesterase 2 | NM_001364178.1 | 49.1% | 46.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1329
- ORF length:
- 1263
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgacgctg atcctggagc ctgcgggccg ctgctgctgg gacgaaccgg 121 tgcgaatcgc cgtgcgcggc ctagccccgg agcagccggt cacgctgcgc gcgtccctgc 181 gcgacgagaa gggcgcgctt ttccaggccc acgcgcgcta ccgcgccgac actcttggcg 241 agctggacct ggagcgcgcg cccgcgctgg gcggcagctt cgcggggctt gagcccatgg 301 ggctgctctg ggccttggag cccgagaaac ctttggtgcg gctggtgaag cgcgacgtgc 361 gaacgccctt ggccgtggag ctggaggtgc tggatggcca cgaccccgac cccgggcggc 421 tgctgtgcca gacgcggcac gagcgctact tcctcccgcc cggggtgcgg cgcgagccgg 481 tgcgcgtggg ccgggtgcga ggcacgctct tcctgccgcc agaacctggg ccctttcctg 541 ggattgtgga catgttcgga actggaggtg gcctgctgga gtatcgggct agtctgctgg 601 ctgggaaggg ttttgctgtg atggctctgg cttattataa ctatgaagac ctccccaaga 661 ccatggagac gctccatctg gagtactttg aagaagccat gaactacttg ctcagtcatc 721 ccgaggtaaa aggtccagga gttgggctgc ttggaatttc caaagggggt gagctctgcc 781 tttccatggc ctctttcctg aagggcatca cggctgctgt cgtcatcaac ggctctgtgg 841 ccaatgttgg gggaacctta cgctacaagg gcgagaccct gccccctgtg ggcgtcaaca 901 gaaatcgcat caaggtgacc aaagatggct atgcagacat tgtggatgtc ctgaacagcc 961 ctttGGAAGG ACCTGACCAG AAGAGCTTCA TTCCTGTGGA AAGGGCAGAG AGCACCTTCC 1021 TGTTCCTGGT AGGTCAGGAT GACCACAACT GGAAGAGTGA GTTCTATGCT AATGAGGCCT 1081 GTAAACGCTT GCAGGCCCAT GGGAGGAGAA AGCCCCAGAT CATCTGTTAC CCAGAGACAG 1141 GGCACTATAT TGAGCCTCCT TACTTCCCCC TGTGTCGGGC TTCCCTGCAT GCCTTGGTGG 1201 GCAGTCCTAT TATCTGGGGA GGGGAGCCCA GGGCTCATGC CATGGCTCAG GTGGATGCTT 1261 GGAAACAACT CCAGACTTTC TTCCACAAAC ACTTGGGTGG CCGCGAGGGG ACAATCCCAT 1321 CAAAAGTGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1381 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1441 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCGCAT TATGGCTAGC ACCCCGAGAC 1501 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt