Construct: ORF TRCN0000474337
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000611.1_s317c1
- Derived from:
- ccsbBroadEn_11440
- DNA Barcode:
- CAAGAGCTACGATCTACTCCCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NR1D2 (9975)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474337
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9975 | NR1D2 | nuclear receptor subfamily ... | XM_006713451.3 | 89.4% | 84.3% | (many diffs) |
| 2 | human | 9975 | NR1D2 | nuclear receptor subfamily ... | NM_005126.5 | 69.2% | 65.5% | (many diffs) |
| 3 | human | 9975 | NR1D2 | nuclear receptor subfamily ... | NM_001145425.1 | 56.3% | 52.8% | (many diffs) |
| 4 | human | 9975 | NR1D2 | nuclear receptor subfamily ... | NR_110524.2 | 22.9% | 1_293del;1518_5338del | |
| 5 | mouse | 353187 | Nr1d2 | nuclear receptor subfamily ... | NM_011584.4 | 60.1% | 57.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1290
- ORF length:
- 1224
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggtgaatgca ggaggtgtga ttgcctatat cagttcttcc agctcagcct 121 caagccctgc ctcttgtcac agtgagggtt ctgagaatag tttccagtcc tcctcctctt 181 ctgttccatc ttctccaaat agctctaatt ctgataccaa tggtaatccc aagaatggtg 241 atctcgccaa tattgaaggc atcttgaaga atgatcgaat agattgttct atgaaaacaa 301 gcaaatcgag tgcacctggg atgacaaaaa gtcatagtgg tgtgacaaaa tttagtggca 361 tggttctact gtgtaaagtc tgtggggatg tggcgtcagg attccactat ggagttcatg 421 cttgcgaagg ctgtaagggt ttctttcgga gaagtattca acaaaacatc cagtacaaga 481 agtgcctgaa gaatgaaaac tgttctataa tgagaatgaa taggaacaga tgtcagcaat 541 gtcgcttcaa aaagtgtctg tctgttggaa tgtcaagaga tgctgttcgg tttggtcgta 601 ttcctaagcg tgaaaaacag aggatgctaa ttgaaatgca aagtgcaatg aagaccatga 661 tgaacagcca gttcagtggt cacttgcaaa atgacacatt agtagaacat catgaacaga 721 cagccttgcc agcccaggaa cagctgcgac ccaagcccca actggagcaa gaaaacatca 781 aaagctcttc tcctccatct tctgattttg caaaggaaga agtgattggc atggtgacca 841 gagctcacaa ggataccttt atgtataatc aagagcagca agaaaactca gctgagagca 901 tgcagcccca gagaggagaa cggattccca aGAACATGGA GCAATATAAT TTAAATCATG 961 ATCATTGCGG CAATGGGCTT AGCAGCCATT TTCCCTGTAG TGAGAGCCAG CAGCATCTCA 1021 ATGGACAGTT CAAAGGGAGG AATATAATGC ATTACCCAAA TGGTCATGCC ATTTGTATTG 1081 CAAATGGACA TTGTATGAAC TTCTCCAATG CTTATACTCA AAGAGTATGT GATAGAGTTC 1141 CGATAGATGG ATTTTCTCAG AATGAGAACA AGAATAGTTA CCTGTGCAAC ACTGGAGGAA 1201 GAATGCATCT GTACAGTGTT CATATTCAGC CAAAATTTCA CCAAAAACAG CAGTACCACA 1261 CCATTTCCAG TTTAAACCAT GAAGTCCTTT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1321 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1381 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACAAGA 1441 GCTACGATCT ACTCCCCCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1501 tgaaagatt