Construct: ORF TRCN0000474344
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003251.1_s317c1
- Derived from:
- ccsbBroadEn_06941
- DNA Barcode:
- CATTGCGAATTACTTGTACTAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SGCD (6444)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474344
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6444 | SGCD | sarcoglycan delta | NM_000337.5 | 99.8% | 100% | 84T>C |
| 2 | human | 6444 | SGCD | sarcoglycan delta | XM_005265966.5 | 99.8% | 100% | 84T>C |
| 3 | human | 6444 | SGCD | sarcoglycan delta | XM_017009723.2 | 99.8% | 100% | 84T>C |
| 4 | human | 6444 | SGCD | sarcoglycan delta | XM_017009724.1 | 99.8% | 100% | 84T>C |
| 5 | human | 6444 | SGCD | sarcoglycan delta | NM_001128209.2 | 99.5% | 99.6% | 0_1insATG;81T>C |
| 6 | human | 6444 | SGCD | sarcoglycan delta | XM_011534621.2 | 99.5% | 99.6% | 0_1insATG;81T>C |
| 7 | human | 6444 | SGCD | sarcoglycan delta | NM_172244.3 | 85.4% | 80.4% | (many diffs) |
| 8 | human | 6444 | SGCD | sarcoglycan delta | XM_005265967.2 | 60.2% | 57.6% | 84T>C;500_501ins73;525_526ins272 |
| 9 | mouse | 24052 | Sgcd | sarcoglycan, delta (dystrop... | NM_011891.4 | 90.1% | 94.1% | (many diffs) |
| 10 | mouse | 24052 | Sgcd | sarcoglycan, delta (dystrop... | XM_006533306.3 | 90.1% | 94.1% | (many diffs) |
| 11 | mouse | 24052 | Sgcd | sarcoglycan, delta (dystrop... | XM_006533307.3 | 90.1% | 94.1% | (many diffs) |
| 12 | mouse | 24052 | Sgcd | sarcoglycan, delta (dystrop... | XM_011249012.1 | 64% | 62.5% | (many diffs) |
| 13 | mouse | 24052 | Sgcd | sarcoglycan, delta (dystrop... | XM_011249013.1 | 58.9% | 62.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 936
- ORF length:
- 870
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gcctcaggag cagtacactc accaccggag caccatgcct ggctctgtgg 121 ggccacaggt atacaaggtg gggatttacg gctggcggaa acgatgcctg tatttctttg 181 tcctgctcct catgatttta atactggtga acttggccat gaccatctgg attctcaaag 241 tcatgaactt cacaattgat ggaatgggaa acctgaggat cacagaaaaa ggtctaaagc 301 tagaaggaga ctctgaattc ttacaacctc tctacgccaa agaaatccag tcccgaccag 361 gtaatgccct gtacttcaag tctgccagaa atgttacagt gaacattctc aatgaccaga 421 ctaaagtgct aactcagctt ataacaggtc caaaagccGT AGAAGCTTAT GGTAAAAAAT 481 TTGAGGTAAA AACTGTTTCT GGAAAATTGC TCTTCTCTGC AGACAATAAT GAAGTGGTAG 541 TAGGAGCTGA AAGATTACGA GTTTTAGGAG CGGAGGGCAC AGTGTTCCCT AAATCTATAG 601 AAACACCTAA TGTCAGGGCA GACCCCTTCA AAGAACTAAG GTTGGAGTCC CCAACCCGGT 661 CTCTAGTGAT GGAGGCCCCA AAAGGAGTGG AAATCAATGC AGAAGCTGGC AATATGGAAG 721 CCACCTGCAG GACAGAGCTG AGACTGGAAT CCAAAGATGG AGAGATTAAG TTAGATGCTG 781 CGAAAATCAG GCTACCTAGA CTGCCTCATG GATCCTACAC GCCTACAGGA ACGAGGCAGA 841 AGGTCTTCGA GATCTGCGTC TGCGCCAATG GGAGATTATT CCTGTCTCAG GCAGGAGCTG 901 GGTCCACTTG TCAGATAAAC ACAAGTGTCT GCCTCTGCCC AACTTTCTTG TACAAAGTGG 961 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1021 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1081 ACATTGCGAA TTACTTGTAC TAGACACGCG TTAAGTCgac aatcaacctc tggattacaa 1141 aatttgtgaa agatt