Construct: ORF TRCN0000474374

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002499.1_s317c1
Derived from:
ccsbBroadEn_01484
DNA Barcode:
AAACCATCTCCGACATGAATGTTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SATB1 (6304)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474374
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6304 SATB1 SATB homeobox 1 NM_001131010.4 100% 100%
2 human 6304 SATB1 SATB homeobox 1 NM_001322872.2 100% 100%
3 human 6304 SATB1 SATB homeobox 1 NM_001322873.2 100% 100%
4 human 6304 SATB1 SATB homeobox 1 NM_001322874.2 100% 100%
5 human 6304 SATB1 SATB homeobox 1 NM_001322875.1 100% 100%
6 human 6304 SATB1 SATB homeobox 1 NM_002971.6 100% 100%
7 human 6304 SATB1 SATB homeobox 1 NM_001195470.3 95.9% 95.9% 1776_1871del
8 human 6304 SATB1 SATB homeobox 1 NM_001322871.2 95.9% 95.9% 1776_1871del
9 human 6304 SATB1 SATB homeobox 1 XM_011533988.3 95.9% 95.9% 1776_1871del
10 human 6304 SATB1 SATB homeobox 1 XM_011533989.2 95.9% 95.9% 1776_1871del
11 human 6304 SATB1 SATB homeobox 1 NM_001322876.2 90.5% 90.5% 0_1ins216
12 human 6304 SATB1 SATB homeobox 1 XM_011533990.3 69.6% 68.6% (many diffs)
13 mouse 20230 Satb1 special AT-rich sequence bi... NM_001163630.1 91.2% 98.2% (many diffs)
14 mouse 20230 Satb1 special AT-rich sequence bi... NM_001163631.1 91.2% 98.2% (many diffs)
15 mouse 20230 Satb1 special AT-rich sequence bi... NM_001163632.1 91.2% 98.2% (many diffs)
16 mouse 20230 Satb1 special AT-rich sequence bi... NM_009122.2 91.2% 98.2% (many diffs)
17 mouse 20230 Satb1 special AT-rich sequence bi... XM_017317363.1 91.2% 98.2% (many diffs)
18 mouse 20230 Satb1 special AT-rich sequence bi... XM_017317364.1 91.2% 98.2% (many diffs)
19 mouse 20230 Satb1 special AT-rich sequence bi... XM_006523928.3 91.2% 98.2% (many diffs)
20 mouse 20230 Satb1 special AT-rich sequence bi... XM_017317365.1 91.2% 98.2% (many diffs)
21 mouse 20230 Satb1 special AT-rich sequence bi... XM_006523923.3 87.7% 94.2% (many diffs)
22 mouse 20230 Satb1 special AT-rich sequence bi... XM_006523924.2 87.7% 94.2% (many diffs)
23 mouse 20230 Satb1 special AT-rich sequence bi... XM_006523925.1 87.7% 94.2% (many diffs)
24 mouse 20230 Satb1 special AT-rich sequence bi... XM_006523926.3 87.7% 94.2% (many diffs)
25 mouse 20230 Satb1 special AT-rich sequence bi... XM_006523927.3 87.7% 94.3% (many diffs)
26 mouse 20230 Satb1 special AT-rich sequence bi... XM_017317366.1 84.9% 91.6% (many diffs)
27 mouse 20230 Satb1 special AT-rich sequence bi... XM_006523929.3 81.7% 87.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2355
ORF length:
2289
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga tcatttgaac gaggcaactc aggggaaaga acattcagaa atgtctaaca
121atgtgagtga tccgaagggt ccaccagcca agattgcccg cctggagcag aacgggagcc
181cgctaggaag aggaaggctt gggagtacag gtgcaaaaat gcagggagtg cctttaaaac
241actcgggcca tctgatgaaa accaacctta ggaaaggaac catgctgcca gttttctgtg
301tggtggaaca ttatgaaaac gccattgaat atgattgcaa ggaggagcat gcagaatttg
361tgctggtgag aaaggatatg cttttcaacc agctgatcga aatggcattg ctgtctctag
421gttattcaca tagctctgct gcccaggcca aagggctaat ccaggttgga aagtggaatc
481cagttccact gtcttacgtg acagatgccc ctgatgctac agtagcagat atgcttcaag
541atgtgtatca tgtggtcaca ttgaaaattc agttacacag ttgccccaaa ctagaagact
601tgcctcccga acaatggtcg cacaccacag tgaggaatgc tctgaaggac ttactgaaag
661atatgaatca gagttcattg gccaaggagt gccccctttc acagagtatg atttcttcca
721ttgtgaacag tacttactat gcaaatgtct cagcagcaaa atgtcaagaa tttggaaggt
781ggtacaaaca tttcaagaag acaaaagata tgatggttga aatggatagt ctttctgagc
841tatcccagca aggcgccaat catgtcaatt ttggccagca accagttcca gggaacacag
901ccgagcagcc tccatcccct gcgcagctct cccatggcag ccagccctct gtccggacac
961ctcttccaaa cctgcaccct gggctcgtat caacacctat cagtcctcaa ttggtcaacc
1021agcagctggt gatggctcag ctgctgaacc agcagtatgc agtgaataga cttttagccc
1081agcagtcctt aaaccaacaa tacttgaacc accctccccc tgtcagtaga tctatgaata
1141agcctttgga gcaacaggtt tcgaccaaca cagaggtgtc ttccgaaatc taccagtggg
1201tacgcgatga actgaaacga gcaggaatct cccaggcggt atttgcacgt gtggctttta
1261acagaactca gggcttgctt tcagaaatcc tccgaaagga agaggacccc aagactgcat
1321cccagtcttt gctggtaaac cttcgggcta tgcagaattt cttgcagtta ccggaagctg
1381aaagagaccg aatataccag gacgaaaggg aaaggagctt gaatgctgcc tcggccatgg
1441gtcctgcccc cctcatcagc acaccaccca gccgtcctcc ccaggtgaaa acagctacta
1501ttgccactga aaggaatggg aaaccagaga acaataccat gaacattaat gcttccattt
1561atgatgagat tcagcaggaa atgaagcgtg ctaaagtgtc tcaagcactg tttgcaaagg
1621ttgcagcaac caaaagccag ggatggttgt gcgagctgtt acgctggaaa gaagatcctt
1681ctccagaaaa cagaaccctg tgggagaacc tctccatgat ccgaaggttc ctcagtcttc
1741ctcagccaga acgtgatgcc atttatgaac aggagagcaa cgcggtgcat caccatGGCG
1801ACAGGCCGCC CCACATTATC CATGTTCCAG CAGAGCAGAT TCAGCAACAG CAGCAGCAAC
1861AGCAACAGCA GCAGCAGCAG CAGCAGGCAC CGCCGCCTCC ACAGCCACAG CAGCAGCCAC
1921AGACAGGCCC TCGGCTCCCC CCACGGCAAC CCACGGTGGC CTCTCCAGCA GAGTCAGATG
1981AGGAAAACCG ACAGAAGACC CGGCCACGAA CAAAAATTTC AGTGGAAGCC TTGGGAATCC
2041TCCAGAGTTT CATACAAGAC GTGGGCCTGT ACCCTGACGA AGAGGCCATC CAGACTCTGT
2101CTGCCCAGCT CGACCTTCCC AAGTACACCA TCATCAAGTT CTTTCAGAAC CAGCGGTACT
2161ATCTCAAGCA CCACGGCAAA CTGAAGGACA ATTCCGGTTT AGAGGTCGAT GTGGCAGAAT
2221ATAAAGAAGA GGAGCTGCTG AAGGATTTGG AAGAGAGTGT CCAAGATAAA AATACTAACA
2281CCCTTTTTTC AGTGAAACTA GAAGAAGAGC TGTCAGTGGA AGGAAACACA GACATTAATA
2341CTGATTTGAA AGACTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
2401CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
2461TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAACCATCTC CGACATGAAT
2521GTTAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)