Construct: ORF TRCN0000474394
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018978.1_s317c1
- Derived from:
- ccsbBroadEn_14760
- DNA Barcode:
- GTGTCCGTATATGGCGTGCTCTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PIK3CB (5291)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474394
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | NM_006219.3 | 99.2% | 19.2% | (many diffs) |
| 2 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_006713659.3 | 99.2% | 19.2% | (many diffs) |
| 3 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_011512895.2 | 99.2% | 19.2% | (many diffs) |
| 4 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_017006619.1 | 99.2% | 19.2% | (many diffs) |
| 5 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_024453596.1 | 92.3% | 14.2% | (many diffs) |
| 6 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XR_002959542.1 | 63.9% | (many diffs) | |
| 7 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_024453597.1 | 62.7% | 6.2% | (many diffs) |
| 8 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_017006621.2 | 61.1% | 7.6% | (many diffs) |
| 9 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | NM_001256045.1 | 54.1% | 6.8% | (many diffs) |
| 10 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | NM_029094.3 | 87.3% | 17.6% | (many diffs) |
| 11 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | XM_011242829.2 | 87.3% | 17.6% | (many diffs) |
| 12 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | XM_011242830.2 | 87.3% | 17.6% | (many diffs) |
| 13 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | XM_006511520.2 | 75% | 4.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 717
- ORF length:
- 651
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg cttcagtttc ctaatgcctc ctgctatggc agacatcctt gacatctggg 121 cggtggattc acagatagca tctgatggct ccatacctgt ggatttcctt ttgcccactg 181 ggatttatat ccagttggag gtacctcggg aagctaccat ttcttatatt aagcagatgt 241 tatggaagca agttcacaat tacccaatgt tcaacctcct tatggatatt gactcctata 301 tgtttgcatg tgtgaatcag actgctgtat atgaggagct tgaagatgaa acacgaagac 361 tctgtgatgt cagacctttt cttccagttc tcaaattagt gacaagaagt tgtgacccag 421 gggaaaaatt agactcaaaa attggagtcc ttataggaaa aggtctgcat gaatttgatt 481 ccttgaagga tcctgaagta aatgaatttc gaagaaaaat gcgcaaattc agcgaggaaa 541 aaatcctgtc acttgtggga ttgtcttgga tggactggct aaaacaaaca tatccaccag 601 agcatgaacc atccatccct gaaaacttag aagataaact ttatggggga aagctcatcg 661 tagctgttca ttttgaaaac tgccagacgt gtttagcttt caagtgtctc ctaatatgaa 721 tcctatcaaa gtaaatgaat tggcaatcca aaaacgtttg actattcatg ggaaggagat 781 gagttagccc ctatgattat gtgttgcagt cagcggagag tagaatatgt ttttggtgat 841 catccactat tcagttccag tatatccgga actgtgtgat gaacagaagc ctgccccatt 901 ttatacttgt ggaatgctgc aagatcaaga aaatgtatga acaagaaatg attgccatag 961 aggctgccat aaatcgaaat tcatctaatc ttcctcttcc attaccacca aagaaaacac 1021 gaattatttc tcatgtttgg gaaaataaca accctttcca aattgtcttg gttaagggaa 1081 ataaacttaa cacagaggaa actgtaaaag ttcatgtcag ggctggtctt tttcatggta 1141 ctgagctcct gtgtaaaacc atcgtaagct cagaggtatc agggaaaaat gatcatattt 1201 ggaatgaacc actggaattt gatattaata tttgtgactt accaagaatg gctcgattat 1261 gttttgctgt ttatgcagtt ttggataaag taaaaacgaa gaaatcaacg aaaactatta 1321 atccctctaa atatcagacc atcaggaaag ctggaaaagt gcattatcct gtagcgtggg 1381 taaatacgat ggtttttgac tttaaaggac aattgagaac tggagacata atattacaca 1441 gctggtcttc atttcctgat gaactcgaag aaatgttgaa tccaatggga actgttcaaa 1501 caaatccata tactgaaaat gcaacagctt tgcatgttaa atttccagag aataaaaaac 1561 aaccttatta ttaccctccc ttcgataaga ttattgaaaa ggcagctgag attgcaagca 1621 gtgatagtgc taatgtgtca agtcgaggtg gaaaaaagtt tcttcctgta ttgaaagaaa 1681 tcttggacag ggatcccttg tctcaactgt gtgaaaatga aatggatctt atttggactt 1741 tgcgacaaga ctgccgagag attttcccac aatcactgcc aaaattactg ctgtcaatca 1801 agtggaataa acttgaggat gttgctcagc ttcaggcgct gcttcagatt tggcctaaac 1861 tgcccccccg ggaggcccta gagcttctgg atttcaacta tccagaccag tacgttcgag 1921 aatatgctgt aggctgcctg cgacagatga gtgatgaaga actttctcaa tatcttttac 1981 aactggtgca agtgttaaaa tatgagcctt ttcttgattg tgccctctct agattcctat 2041 tagaaagagc acttggtaat cggaggatag ggcagtttct attttggcat cttaggtcag 2101 aagtgcacat tcctgctgtc tcagtacaat ttggtgtcat ccttgaagca tactgccggg 2161 gaagtgtggg gcacatgaaa gtgctttcta agcaggttga agcactcaat aagttaaaaa 2221 ctttaaatag tttaatcaaa ctgaatgccg tgaagttaaa cagagccaaa gggaaggagg 2281 ccatgcatac ctgtttaaaa cagagtgctt accgggaagc cctctctgac ctgcagtcac 2341 ccctgaaccc atgtgttatc ctctcagaac tctatgttga aaagtgcaaa tacatggatt 2401 ccaaaatgaa gcctttgtgg ctggtataca ataacaaggt atttggtgag gattccagtt 2461 gggagtgatt tttaaaaaat ggtgatgatt tacgaccaga taatgttgac actcccaaat 2521 gttgcgctgg atgattttac tctggaaaga agctggttgg attcttcgga tgttgcctta 2581 tggctgttag caaacaggag atcgctctgg cntcattgaa gttgtgagca cctctgaaac 2641 aattgctgac attcagctga acagtagcaa tgtggctgct gcagcagcct tcaacaaaga 2701 tgcccttctg aactggctta aagaatacaa ctctggggat gacctggacc gagccattga 2761 ggaatttaca ctgtcctgtg ctggctactg tgtagcttct tatgtccttg ggattggtga 2821 cagacatagt gacaacatca tggtcaaaaa aactggCCAG CTCTTCCACA TTGACTTTGG 2881 ACATATTCTT GGAAATTTCA AATCTAAGTT TGGCATTAAA AGGGAGCGAG TGCCTTTTAT 2941 TCTTACCTAT GATTTCATCC ATGTCATTCA ACAAGGAAAA ACAGGAAATA CAGAAAAGTT 3001 TGGCCGGTTC CGCCAGTGTT GTGAGGATGC ATATCTGATT TTACGACGGC ATGGGAATCT 3061 CTTCATCACT CTCTTTGCGC TGATGTTGAC TGCAGGGCTT CCTGAACTCA CATCAGTCAA 3121 AGATATACAG TATCTTAAGG ACTCTCTTGC ATTAGGGAAG AGTGAAGAAG AAGCACTCAA 3181 ACAGTTTAAG CAAAAATTTG ATGAGGCGCT CAGGGAAAGC TGGACTACTA AAGTGAACTG 3241 GATGGCCCAC ACAGTTCGGA AAGACTACAG ATCTTTGCCA ACTTTCTTGT ACAAAGTGGT 3301 TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC 3361 TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 3421 GTGTCCGTAT ATGGCGTGCT CTTTACGCGT TAAGTCgaca atcaacctct ggattacaaa 3481 atttgtgaaa gatt