Construct: ORF TRCN0000474394

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018978.1_s317c1
Derived from:
ccsbBroadEn_14760
DNA Barcode:
GTGTCCGTATATGGCGTGCTCTTT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PIK3CB (5291)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474394
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5291 PIK3CB phosphatidylinositol-4,5-bi... NM_006219.3 99.2% 19.2% (many diffs)
2 human 5291 PIK3CB phosphatidylinositol-4,5-bi... XM_006713659.3 99.2% 19.2% (many diffs)
3 human 5291 PIK3CB phosphatidylinositol-4,5-bi... XM_011512895.2 99.2% 19.2% (many diffs)
4 human 5291 PIK3CB phosphatidylinositol-4,5-bi... XM_017006619.1 99.2% 19.2% (many diffs)
5 human 5291 PIK3CB phosphatidylinositol-4,5-bi... XM_024453596.1 92.3% 14.2% (many diffs)
6 human 5291 PIK3CB phosphatidylinositol-4,5-bi... XR_002959542.1 63.9% (many diffs)
7 human 5291 PIK3CB phosphatidylinositol-4,5-bi... XM_024453597.1 62.7% 6.2% (many diffs)
8 human 5291 PIK3CB phosphatidylinositol-4,5-bi... XM_017006621.2 61.1% 7.6% (many diffs)
9 human 5291 PIK3CB phosphatidylinositol-4,5-bi... NM_001256045.1 54.1% 6.8% (many diffs)
10 mouse 74769 Pik3cb phosphatidylinositol-4,5-bi... NM_029094.3 87.3% 17.6% (many diffs)
11 mouse 74769 Pik3cb phosphatidylinositol-4,5-bi... XM_011242829.2 87.3% 17.6% (many diffs)
12 mouse 74769 Pik3cb phosphatidylinositol-4,5-bi... XM_011242830.2 87.3% 17.6% (many diffs)
13 mouse 74769 Pik3cb phosphatidylinositol-4,5-bi... XM_006511520.2 75% 4.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
717
ORF length:
651
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtg cttcagtttc ctaatgcctc ctgctatggc agacatcctt gacatctggg
121cggtggattc acagatagca tctgatggct ccatacctgt ggatttcctt ttgcccactg
181ggatttatat ccagttggag gtacctcggg aagctaccat ttcttatatt aagcagatgt
241tatggaagca agttcacaat tacccaatgt tcaacctcct tatggatatt gactcctata
301tgtttgcatg tgtgaatcag actgctgtat atgaggagct tgaagatgaa acacgaagac
361tctgtgatgt cagacctttt cttccagttc tcaaattagt gacaagaagt tgtgacccag
421gggaaaaatt agactcaaaa attggagtcc ttataggaaa aggtctgcat gaatttgatt
481ccttgaagga tcctgaagta aatgaatttc gaagaaaaat gcgcaaattc agcgaggaaa
541aaatcctgtc acttgtggga ttgtcttgga tggactggct aaaacaaaca tatccaccag
601agcatgaacc atccatccct gaaaacttag aagataaact ttatggggga aagctcatcg
661tagctgttca ttttgaaaac tgccagacgt gtttagcttt caagtgtctc ctaatatgaa
721tcctatcaaa gtaaatgaat tggcaatcca aaaacgtttg actattcatg ggaaggagat
781gagttagccc ctatgattat gtgttgcagt cagcggagag tagaatatgt ttttggtgat
841catccactat tcagttccag tatatccgga actgtgtgat gaacagaagc ctgccccatt
901ttatacttgt ggaatgctgc aagatcaaga aaatgtatga acaagaaatg attgccatag
961aggctgccat aaatcgaaat tcatctaatc ttcctcttcc attaccacca aagaaaacac
1021gaattatttc tcatgtttgg gaaaataaca accctttcca aattgtcttg gttaagggaa
1081ataaacttaa cacagaggaa actgtaaaag ttcatgtcag ggctggtctt tttcatggta
1141ctgagctcct gtgtaaaacc atcgtaagct cagaggtatc agggaaaaat gatcatattt
1201ggaatgaacc actggaattt gatattaata tttgtgactt accaagaatg gctcgattat
1261gttttgctgt ttatgcagtt ttggataaag taaaaacgaa gaaatcaacg aaaactatta
1321atccctctaa atatcagacc atcaggaaag ctggaaaagt gcattatcct gtagcgtggg
1381taaatacgat ggtttttgac tttaaaggac aattgagaac tggagacata atattacaca
1441gctggtcttc atttcctgat gaactcgaag aaatgttgaa tccaatggga actgttcaaa
1501caaatccata tactgaaaat gcaacagctt tgcatgttaa atttccagag aataaaaaac
1561aaccttatta ttaccctccc ttcgataaga ttattgaaaa ggcagctgag attgcaagca
1621gtgatagtgc taatgtgtca agtcgaggtg gaaaaaagtt tcttcctgta ttgaaagaaa
1681tcttggacag ggatcccttg tctcaactgt gtgaaaatga aatggatctt atttggactt
1741tgcgacaaga ctgccgagag attttcccac aatcactgcc aaaattactg ctgtcaatca
1801agtggaataa acttgaggat gttgctcagc ttcaggcgct gcttcagatt tggcctaaac
1861tgcccccccg ggaggcccta gagcttctgg atttcaacta tccagaccag tacgttcgag
1921aatatgctgt aggctgcctg cgacagatga gtgatgaaga actttctcaa tatcttttac
1981aactggtgca agtgttaaaa tatgagcctt ttcttgattg tgccctctct agattcctat
2041tagaaagagc acttggtaat cggaggatag ggcagtttct attttggcat cttaggtcag
2101aagtgcacat tcctgctgtc tcagtacaat ttggtgtcat ccttgaagca tactgccggg
2161gaagtgtggg gcacatgaaa gtgctttcta agcaggttga agcactcaat aagttaaaaa
2221ctttaaatag tttaatcaaa ctgaatgccg tgaagttaaa cagagccaaa gggaaggagg
2281ccatgcatac ctgtttaaaa cagagtgctt accgggaagc cctctctgac ctgcagtcac
2341ccctgaaccc atgtgttatc ctctcagaac tctatgttga aaagtgcaaa tacatggatt
2401ccaaaatgaa gcctttgtgg ctggtataca ataacaaggt atttggtgag gattccagtt
2461gggagtgatt tttaaaaaat ggtgatgatt tacgaccaga taatgttgac actcccaaat
2521gttgcgctgg atgattttac tctggaaaga agctggttgg attcttcgga tgttgcctta
2581tggctgttag caaacaggag atcgctctgg cntcattgaa gttgtgagca cctctgaaac
2641aattgctgac attcagctga acagtagcaa tgtggctgct gcagcagcct tcaacaaaga
2701tgcccttctg aactggctta aagaatacaa ctctggggat gacctggacc gagccattga
2761ggaatttaca ctgtcctgtg ctggctactg tgtagcttct tatgtccttg ggattggtga
2821cagacatagt gacaacatca tggtcaaaaa aactggCCAG CTCTTCCACA TTGACTTTGG
2881ACATATTCTT GGAAATTTCA AATCTAAGTT TGGCATTAAA AGGGAGCGAG TGCCTTTTAT
2941TCTTACCTAT GATTTCATCC ATGTCATTCA ACAAGGAAAA ACAGGAAATA CAGAAAAGTT
3001TGGCCGGTTC CGCCAGTGTT GTGAGGATGC ATATCTGATT TTACGACGGC ATGGGAATCT
3061CTTCATCACT CTCTTTGCGC TGATGTTGAC TGCAGGGCTT CCTGAACTCA CATCAGTCAA
3121AGATATACAG TATCTTAAGG ACTCTCTTGC ATTAGGGAAG AGTGAAGAAG AAGCACTCAA
3181ACAGTTTAAG CAAAAATTTG ATGAGGCGCT CAGGGAAAGC TGGACTACTA AAGTGAACTG
3241GATGGCCCAC ACAGTTCGGA AAGACTACAG ATCTTTGCCA ACTTTCTTGT ACAAAGTGGT
3301TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC
3361TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA
3421GTGTCCGTAT ATGGCGTGCT CTTTACGCGT TAAGTCgaca atcaacctct ggattacaaa
3481atttgtgaaa gatt

Download FASTA (ORF) (Full)